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We narrowed to 707 results for: BASE

Showing: 621 - 640 of 707 results
  1. AAV ddPCR Titration

    Type
    Protocol
    ...The dilution series outlined in this protocol are based on an AAV titer range of 5E+12–5E+13 GC/mL, where...
  2. March for Science

    Type
    Blog Post
    ... the meaning of the word ‘fact’. Policy must be based on our most accurate interpretation of the world...
  3. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...fail to recapitulate the true steric and charge-based nature of the phosphoryl-modification; they are ...appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications, identifies...
  4. CRISPR Library Amplification

    Type
    Protocol
    ... but care must be taken to perform adequate NGS based QC to ensure no change in representation compared...
  5. Lentivirus ddPCR Titration

    Type
    Protocol
    ...The dilution series outlined in this protocol is based on an LV titer range of 1E+05–1E+09 TU/mL, where...
  6. Antibodies 101: Validation

    Type
    Blog Post
    ...same conditions. Example: You want to sort cells based on the expression of a new receptor that is of interest...attempts by other means, such as through antibody databases like Antibodypedia or through your antibody provider...
  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519.e17. ...
  8. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila... recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced. Cre-mediated...repair templates (ie. PCR-derived dsDNA) with 30-60 basepair homology arms to knock in large epitopes, such...
  9. Deep Dive: qPCR

    Type
    Blog Post
    ... to multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to five or six reactions...
  10. Immunology Research Plasmids and Resources

    Type
    Collection
    ...Immunology Research Genes have been grouped into tables based on the Gene Lists from Immport . Click the links...grouped in to the CC, CXC, XC, and CX3C families based on conserved cysteine residues. Chemokines recruit...
Showing: 621 - 640 of 707 results