We narrowed to 802 results for: Clu

1. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

   ...will focus on assays to detect NHEJ. These assays include a forward and reverse primer to amplify the target...

2. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   ...DREADD (Kügler et al., 2003), which can then be exclusively activated by the small molecule clozapine N-oxide...

3. Early Career Researcher Toolbox: Social Media for Scientists

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   Blog Post

   ...And the blog writing tips are elements I try to include in each post I write for the Addgene blog. 5. "...

4. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   Blog Post

   ...is most often used, but other selection markers include vermillion (another gene controlling eye color)...

5. How Addgene Creates Content

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   Blog Post

   ...team, we identified what applications should be included and what basic information someone new to antibodies...

6. Plasmids 101: Screens vs. Selections

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   Blog Post

   ...Things to consider when selecting an assay system include: How specific is it? Is it high throughput? Will...

7. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   Blog Post

   ...described in this post in the graphic below - including some simple comparisons. The easy multiplexing...

8. CRISPR 101: Non-Homologous End Joining

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   Blog Post

   ...NHEJ has a vast toolbox of processing factors, including polymerases (Pol μ and Pol λ), nucleases (Artemis...

9. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   Blog Post

   ...Further Reading: For more information about SAM, including a tool for getting optimized guide sequences for...

10. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    Blog Post

    ...Mike recommends that the team should initially include a visionary, a science-minded individual, and a...

11. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post

    ...for one or both of these criteria. If you can, include people from different labs, departments or organizations...

12. The time and cost required to make a plasmid

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    Blog Post

    ...make a plasmid, which came out to $115/plasmid, excluding any restriction enzymes or vector purchases (Table...

13. Plasmids 101: The protein expression toolbox

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    Blog Post

    ...once the ligand is removed. Degron tag systems include small molecule assisted shut off (SMASh), auxin-inducible...

14. All in a Twist: dsRNA

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    Blog Post

    ...been documented in several autoimmune disorders, including some forms of lupus and rheumatoid arthritis. ...

15. What's New in CRISPR - November 2021

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    Blog Post

    ...and human cells using various delivery methods, including plasmid, ribonucleoprotein, and AAV.  Read the...

16. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post

    ...protein-coupled receptor, one of the Designer Receptor Exclusively Activated by Designer Drugs (DREADDs) (Armbruster...

17. dTAG - You're it!

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    Blog Post

    ...distinct advantages over pharmacological inhibitors including rapidly depleting a protein-of-interest, increasing...

18. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    Blog Post

    ...to nine proteins in ten ddifferent cell types, including difficult-to-manipulate primary cells, achieving...

19. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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    Blog Post

    ...skills that Susan  finds important for her work include: “Listening. Evaluating evidence. Critical thinking...

20. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post

    ...Under normal growth conditions, both variants are excluded from the vacuole and emit red and green fluorescence...