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We narrowed to 957 results for: Cre

Showing: 621 - 640 of 957 results

  2. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...using the plexWell technology to easily and quickly create Illumina sequencing libraries. From start to finish...ask the depositing laboratory to review the discrepancies. If the depositing laboratory confirms that ...-Cas9 knockout and transcriptional activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038...

  3. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...growth or decline of interest in a field over time. Creating an alert for a search term helps automate your...is not a traditional search engine. Instead, you create your own research feed by entering keywords, concepts...new papers that contain these terms. You can also create intersects in your feed which means only papers...

  4. 6 Tips for Grant Writing

    Type
    Blog Post
    ...tips to improve your grant writing and hopefully increase your success rate. 1. Submit your grant early...are unlikely to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor...taxes or charitable donations) so there is an increasing realization that, if we want people to keep contributing...

  5. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...Mammalian expression plasmids are primarily used to create mRNA and the commonly used mammalian terminators...below) releases the mRNA from the polymerase and creates a free 3' end which is now available for polyadenylation...Internal Polyadenylation Signal Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes...

  6. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...Victoria, CA Over the decades science has become increasingly restricted to academic and industrial labs, ...research. This interest manifested itself in the creation of Hackerspaces and Makerspaces back in 2007, ... make accounts with  Addgene. We now have the incredible CRISPR/cas9 tools at our disposal to to modify...

  7. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...disulfide bridges, glycosylation and acetylation to create functional, stable proteins. Protein expression...scientists have taken advantage of the natural secretion of biochemicals and proteins through plant roots... Tagging recombinant proteins with a naturally secreted plant peptide allows for easier access and purification...

  8. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...parallel because the risk of accidental errors increases. In addition to human error, fragments of DNA ...to the reference sequence of the plasmid used to create the viral prep. In a clean viral prep, like AAV...control process! The images in this blog post were created using Snapgene and Geneious software. References...

  9. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...as 30% of all cancers, especially those of the pancreas and colon. Despite the prevalence of Ras mutations...he or she can use to study it (as seen in the screenshot below). Currently, the page includes interactive... Tackling Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used...

  10. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    .... At Addgene, we use blastn to identify any discrepancies in Sanger sequences, such as mismatches, deletions...determine the potential effect of any nucleotide discrepancies. The blastp and blastx programs are optimized...plasmids instead! All BLAST images are modified screen shots from the NCBI BLAST website. Additional Resources...

  11. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...a double-edged sword. An antibody’s large size creates more distance between the target antigen and the...also allows for better tissue penetration and decreases the distance between a fluorescent tag and the...HCab antibodies from its serum, and meticulously screening, optimizing, and characterizing several anti-mouse...

  12. DNA Purification Without a Kit

    Type
    Blog Post
    ...expense and requirement for lab equipment. Plus they create plastic waste. Kits can also have the annoying ...and filters can be stacked together in series to increase binding capacity. Figure 1: Purify DNA using...Additionally, while commercial plasmid prep kits come in discreet sizes (mini, midi, maxi, giga) which requires ...

  13. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...overexpression of protein fusions to gene-edited cells can decrease the number of fluorophores, compromising the photon...improved fluorophores are making chemical dyes increasingly attractive and accessible to cell biologists—and...fluorogenicity, photoactivation etc.—can further increase the photon budget and allow us to push further...

  14. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...and multiple gRNAs into separate AAV vectors, increasing overall packaging capacity but necessitating ...efficiency of AAV-SaCas9 in vivo, the Zhang lab created an all-in-one SaCas9 and sgRNA construct using ...histologically normal and liver injury markers were not increased compared to a control AAV-GFP. Not only did the...

  15. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...1998). As the copy number of the plasmid increases, increased amounts of these antisense RNAs inhibit ...inhibit its own transcription. As copy number increases, RepA transcription is repressed and replication...

  16. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...that can function in more than one host species. Created with BioRender.com. How are shuttle vectors...viral oris to drive amplification, which helps increase cellular uptake, but this approach requires compatible...eukaryotes. Conclusion I still think it’s pretty incredible that my cells “speak” the same molecular language...

  17. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...proteins cooperatively can separate the DNA strands. Created with BioRender.com. Once DnaA breaks the local...helicases to continue unwinding the strands. This creates two replication forks that move in opposite directions...pseudoknot from folding, blocking replication. Created with BioRender.com. Plasmids under stringent...

  18. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...just might personally hand your credentials to HR, greatly increasing your chances of getting a phone ...can use to better prepare for your career, and increase your chances of landing that next coveted job!...

  19. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...expression transposases leading to increased integration frequency. (C) Increasing rounds of transfer on transposition-inducing...chromosomal integration of cargo without using screening markers. Capability to construct a strain library...

  20. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...each project. This creates accountability on the part of each developer and creates trust amongst the ... Addgene grows and evolves, we will be here to increase all of our teams’ efficiency and productivity....
Showing: 621 - 640 of 957 results