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We narrowed to 1,015 results for: LEA

Showing: 621 - 640 of 1015 results

  1. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array of topics pertinent...Plasmids 101 2nd ed, the 3rd edition has now been released), and have started providing recorded interviews...Resources at Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository ...

  2. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...genome engineering methods for C. elegans were released, signaling a new era in C. elegans research. Homology...on the first attempt for 6/7 genes. The data are clear - the SEC-based system is both simple to use and...rapid, and user-friendly system represents another leap forward for the CRISPR/Cas9 revolution!   References...

  3. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...move, such as T lymphocytes. Griesbeck and his colleagues began by isolating a calcium-binding protein,... also points out that the approach he and his colleagues took to optimize sensitivities can now be applied...calcium, requiring greater sensitivity. He and his colleagues came up with a solution: they devised self-associating...

  6. The MTA at Addgene

    Type
    Blog Post
    ...depends on things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar...how the shared reagents should be used. Setting clear expectations from the beginning makes the sharing...sharing process smoother, more consistent, and more pleasant for everyone, which in turn encourages people ...

  7. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...dysregulation. A targeted TF screening approach can not only lead to a better understanding of disease onset, but ...isoforms. Due to the size of the collection, we’re releasing individual plasmids in batches. If there’s something....2017.016. Additional Resources on the Addgene Blog Learn More about Lentivirus Figure out How to Get the ...

  8. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...results as soon as the gels ran.” Dr. Ellis tries to lead his team as a scientists and a friend. George Church...3 for tips on how to develop your role as a lab leader, and then on to Chapter 4 for advice on staffing... labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early Career Scientists...

  9. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...This is just a primer so that you are aware of at least some of the possibilities. Basically, if you can...between pathogens and their hosts. It’s not always clear which side he roots for. You can try to convince...PMC3172133. Additional Resources on the Addgene Blog Learn about Using Cre in Drosophila Read Other Genome ...

  10. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ... Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder, Melina Fan, we created...Production webpage). While the new team took the lead on operationalizing viral production and distribution...Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral Service in Your Research...

  12. Antibodies 101: Isotypes

    Type
    Blog Post
    ...immune response. There are three main pathogen clearing functions or effector functions of the constant...are two IgA subclasses: IgA1 and IgA2.  You can learn more about isotype classes in our Antibodies 101...NBK27162/ Additional resources on the Addgene blog Learn about the advantages and disadvantages of using ...

  13. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses can transduce both dividing... small enough to enter the nucleus through the nuclear pore complex. This feature may be useful for the...stimulates the expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -R -U5 LTR...

  14. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...our Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one..., McPhee DA, Verity E, Dyer WB, Wesselingh SL, Learmont J, Sullivan JS, Roche M, Zaunders JJ, Gabuzda ...Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral...

  15. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... specificity of monoclonal antibodies, they also lead to more reproducible experimental results. When ...NBK100203/ Additional resource on the Addgene blog Learn more about research applications for antibodies ...Studies Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies for immunotherapy Resource...

  16. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...receptor-transcription factor fusion. TEV protease cleave the transcription factor from the receptor, which... in chemogenetics  While there’s still a lot to learn about GPCR signaling, researchers in the meantime...02775-8 Additional resources on the Addgene blog Learn more about TRUPATH Read our quick guide to chemogenetics...

  17. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...forward-looking. Embrace it, and let your personality leap out of the page. Finally, as previously mentioned...the recommender knows you the better, and their leadership status in their current field of expertise augments...But you create a network of friends, and future colleagues that can help pull one another up while you are...

  18. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...search tool updates. Now, let’s take a look at the least visible part behind these changes: the backend technology...engine! Additional resources from the Addgene blog Learn about other features on Addgene's website Find a...step-by-step guide to using Addgene's new search Read to learn more about why we redesigned our homepage and mascot...

  19. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...’s wasting everybody’s time if you don’t. Add a clear cover letter to your submission, briefly summarizing...house style and prerequisites of the journal. Ask colleagues (preferably native English speakers) to proofread...that all required information is provided. The clearer you write your paper and the more information you...
Showing: 621 - 640 of 1015 results