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  1. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying all the major equipment...their success. Therefore, assume positive intent. Sometimes, “no we don’t have the funds,” really means they...
  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...technology, and the user may need to optimize numerous parameters to generate the desired edit. The wrench: Gene...universal scoring system for selecting a gRNA, as the method of producing the guide (synthetic, in vitro transcription...
  3. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...milestone for the molecular biologist was to devise methods to manipulate it. The term genetic engineering ...history of CRISPR Read about other genome editing methods Resources on Addgene.org Visit our CRISPR guide...
  4. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... constructs that compose genetic circuits and metabolic pathways. For many MoClo systems, the individual...interested in recombinant protein purification and metabolic engineering. If instead you want to build genetic...
  5. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...through multiple cell divisions, indicating that this method produces stable edits. However, this system is ... effects, ABEs also compare favorably to other methods. In a head-to-head comparison with Cas9, Cas9 modified...
  6. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...tip #1: Glove trick Putting on lab gloves can sometimes be annoying. To make it easier, try this quick...
Showing: 621 - 640 of 844 results