We narrowed to 928 results for: Mpl;

...want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add...transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified Cas9 protein and in vitro transcribed...transcribed gRNA are combined to form a Cas9-gRNA complex and delivered to cells using cationic lipids. Transient...

...happy to upload your newest resources. Here is an example of a plasmid with a downloadable protocol listed...have a record of your work. This feature further simplifies lab work if you like to bring your phone in the...of checking off each step as you painstakingly complete your experiment – one small victory in a long ...

...allow people to, for example, knock out a gene with simultaneous complementation, with a knockout and ...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...

...getting-started guide for BLAST, which includes a simple explanation of the different BLAST programs, databases...consistently run our blastx searches with the “Low complexity regions” filter unchecked so that these regions...benefit is that blastp searches do not filter low complexity regions by default, meaning that you do not have...

...sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry(orange..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...same reference genomes and libraries. Further complicating the issue is the long and ugly history of reporting...

...blots, IP, and affinity purification. Arguably the simplest affinity tag is the polyhistidine (His) tag. Small... a few time-tested tags and fusion proteins as examples. The tables list more common tags than are described...Biotechnology Journal 2012 , (7): 620-634. Full Text A complete list of free handbooks for protein purification...

... array of biological disciplines and scientists employ GFP for a wide number of functions, including: ...importance of the entire GFP protein, which is almost completely required to maintain fluorescent activity; very...modified ES cells, which can then be used for implantation and generation of transgenic mice. Purification...

...to be managing. Being reminded of the seemingly simple actions we can take to help ourselves can be an... in the family to have to miss something – for example, say no to some of those birthday parties. Tell...time. For me, that’s been volunteer work at our temple, my kids’ school, and with professional organizations...

...Eventually I learned that it’s practical because there simply isn’t enough time to read every single paper. I... read the original reference for the data. For example, we summarized how frequently each member of a ...original paper if you wanted to learn more. Another example: we made a schematic of all the proteins in the...

...also operates with a single RuvC-like domain. In simple terms, Cas9 cuts DNA using two "scissors" (one ...a 20-nucleotide (nt) guide sequence that is complementary to the DNA target. The 20-nt guide region binds...Oligo 2: Add 'ggcc' to the 5′ end of your reverse complement guide (see Figure 1). Perform standard phosphorylation...

...pBAD-bARGSer-AxeTxe, gas vesicle expression in E. coli is easy: simply grow cells in Lennox LB (10 g/L tryptone, 5 g/L...improved purity and contrast. For in vivo experiments, simply induce injected engineered cells via regular intraperitoneal...a chemical transfection reagent (e.g. PEI-MAX). Simply cotransfect a mixture of 1248 ng of CMV-gvpA-IRES-BFP...

...subunit of DNA gyrase and trapping it in the cleaved complex. This results in DNA breakage and cell death. Conversely...often lost without plasmid addiction systems. For example, Cara Morin and James Kaper used the hok/sok system...K. Burley. "Crystal structure of the MazE/MazF complex: molecular bases of antidote-toxin recognition....

...using viral LTRs as RNAP II promoters is commonly employed in lentiviral and retroviral constructs and we...speaking, promoters in bacteria are less diverse and complex, having fewer parts than those in eukaryotic cells...human ubiquitin C gene Constitutive  As the name implies, this promoter is ubiquitous. human beta actin...

...signaling — think of a mouse with an LED brain implant or a worm wiggling back and forth under blue light...have various disadvantages and limitations – for example, microtubule-destabilizing drugs are impossible... embryo (Aljiboury et al., 2023). It’s a great example of how optogenetics allows you to disrupt and study...

...longer RNAs (such as mRNAs for gene expression). Examples of commonly used RNA pol II promoters for Cas9...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...delivery and expression in planta using chitosan-complexed single-walled carbon nanotube carriers. Nat Nanotechnol...

...convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918...BL21 strain and derivatives are the most common examples of the E. coli B strain. Common E. coli strains...vectors. Used by Addgene for pooled library amplification. F' mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96...

...engineered ESCs), and chimeric adults mated to produce completely transgenic offspring. Although powerful, this... finally, we have a technology, CRISPR, that is simple in concept, straightforward to use, and robust ...enrichment and selection offered by mouse ESCs. For example, we often screen only 15-25 mice when generating...

...with and be inspired by the mentor’s successful example. For evidence of this, you needn’t look any further...cancer. She has guided me in my next steps. For example, how to get a fellowship to travel abroad, how ... a post-program self-assessment, participants completed follow-up self assessments and program evaluations...

... NanoLuc® Luciferase-based tools are fantastic examples of technologies that can accelerate your research...endogenous protein tagging as it is only 19 kDa. An example is the CRISPaint-NLuc construct (Plasmid #67178...Another challenge to using FRET sensors comes when employing optogenetic regulators to initiate the event being...

...are working with proto-oncogenes or with human samples that have not been tested for HIV.  If not using...must be chosen carefully. Often researchers want a simple set-up where the gene of interest is expressed ...experience in transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly...