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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 910 results for: Nes

Showing: 621 - 640 of 910 results

  1. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...postdocs do to ensure the advisors they choose will be ones that help them succeed after the training period... familiarity and confidentiality to ensure more honest answers. Ask people who work in the labs next door...

  3. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...partially reprogrammed and re-expressed fibroblast genes. Most cells ended up taking the ‘dead-end’ path....CellTag is a combinatory cell-indexing tool that combines scRNA-seq and lineage tracking to track the dynamics...

  5. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...with different properties (for example, overall brightness or on/off kinetics) that makes them suitable ...pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant (pAAV.hSyn-FLEX.iGABASnFR....

  6. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...transduction methods. Some ‘difficult to transfect’ cells lines are amenable to transduction, making it a logical...Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene...

  7. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...derived were tested for specificity in knock-out lines or animals.  Addgene will continue to add more antibodies...antibodies, for neuroscience research and other disciplines, over time. The resource may also expand to include...

  8. RUBY-Red Siliques

    Type
    Blog Post
    ...fluorescent proteins. Selection using resistance genes usually involves killing the untransformed seedlings...then green offspring can be selected instead of red ones. Other innovative uses of RUBY are possible, for...

  9. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ... and genomic DNA in a variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like...certain guide sequences - using 5 guides/gene for 10 genes, they did not observe differences in cleavage efficiency...

  11. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...going forward. This is not just a question of being honest and straightforward, it’s an important part of ... various different versions of checklists and guidelines to help authors understand what they are expected...

  12. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...the gRNA target site. In addition to increased brightness, they also found that the signal to noise ratio... observing low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly,...

  14. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn profile...She supported me and answered my questions and honestly, she was the only person who really understood...

  15. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ... help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice...19. You can also give back by supporting local businesses (ex: purchasing a gift card to use later or ordering...

  16. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...experimental model. In fact, approximately 25% of yeast genes have a human counterpart, enabling study of human-relevant...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

  17. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...performed using traditional 2D assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in .... P., Herskovitz, J., Goletz, T. J., Zhou, L., Moxness, M., Flynn, G. C., Narhi, L. O., & Jawa, V. (2016...

  18. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ... know that science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for...applying to a structured program, they likely have guidelines to consult on this topic.   Etiquette...

  20. Cultivating Community Science at BosLab

    Type
    Blog Post
    ... with audiovisual media, software engineering, business consulting, mathematics, and more. That’s right...BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge with the community...
Showing: 621 - 640 of 910 results