We narrowed to 928 results for: POR

... assay, the pseudoviruses express a luciferase reporter which is used as a readout for infection of ACE2...neutralize the virus,” says Balazs, emphasizing the importance of the second vaccine dose in mounting a robust...

... new services. “We appreciate NIH’s vision and support for this project. This grant will allow Addgene... part of this initiative! The project will be supported by a NIH BRAIN Initiative grant (NS119916). The...

...encodes it. That DNA sequence then needs to be incorporated into a host cell (through a process called transformation...FD-CF reactions affordable to many schools. Most importantly, experiments for FD-CF reactions can be fun and...

...assembly. What makes it so forgiving, is the incorporation of a 3’-5’ exonuclease in the reaction mixture...assembly approaches, and opens the door for new opportunities in the field.  Gibson Assembly® is a registered...

...proteins consuming molecular fuel sources. While the importance of understanding these complex processes can’...

... combined with the tracrRNA forms the scaffold portion of a gRNA and the “spacer region” forms the target...

... of being honest and straightforward, it’s an important part of being a “good scientific citizen” and ...Press journals, including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from...

...the right candidate for the job. How can you incorporate the skills developed during graduate school into...whether to stay in academia or not, it is even more important to start early. Think about your plan for the ...

...Gerald Schwank, first author of the cystic fibrosis report. “This is a big advantage over induced pluripotent...and many others. “Those off-target effects are important,” she said. “We also really need to get a handle...

...other areas of scientific research. We continue to support those involved in COVID-19 research, but our new...make science a better place. You will receive opportunities to collaborate with other scientists and to ...

...be shared with others. The Web Importer plug-in lets you directly import papers from web pages and academic...

...recommends that you sequence any portion of the plasmid that is important for your experiments. There are... Digest Introduction Sequence verification of important plasmid features (such as the gene/insert, fusion...sequences the plasmid to verify tags, mutations and a portion of the insert, but we do not sequence the entire...an example of a trace file from a high-quality portion of a sequencing reaction: Sequence near the beginning...unreliable. This is an example of a trace file from a portion of a sequencing reaction with high background: ...

...from the mol bio side you’re sure to have many opportunities to learn about and develop technologies that...FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a ...

...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using... substrate is required for imaging, and it is important to verify that this substrate does not alter cellular...

... information on each cancer clinical sequencing report includes rare variants that haven’t been characterized...MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319. PubMed Central PMCID...

...detection limits of about 5% mutant targets have been reported for mismatch nuclease assays (2, 6, 7). Ability...nuclease assays are completely “blind” to this very important difference (Figure 3). If you are interested...

...NPR, articles in magazines like Fast Company, opportunities to speak at conferences, and offers for postdoc...can fit into a researcher’s work day. It’s also important to remember that it’s not the amount of time you...

...sequence flexibility indicates that gRNAs could be incorporated into these constructs without compromising cleavage...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

...process involve repairing chromosomal DSBs. It is important to know that cells possess two major repair pathways...chance of causing a frameshift mutation. Of some importance, the deletion can be less heterogeneous when ...

... cell membrane. Heterotrimeric G proteins are important molecular switches in signal transduction pathways...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ...