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We narrowed to 921 results for: Por

Showing: 621 - 640 of 921 results

  3. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...assembly. What makes it so forgiving, is the incorporation of a 3’-5’ exonuclease in the reaction mixture...assembly approaches, and opens the door for new opportunities in the field.  Gibson Assembly® is a registered...

  4. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... of being honest and straightforward, it’s an important part of being a “good scientific citizen” and ...Press journals, including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from...

  5. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...Gerald Schwank, first author of the cystic fibrosis report. “This is a big advantage over induced pluripotent...and many others. “Those off-target effects are important,” she said. “We also really need to get a handle...

  6. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...encodes it. That DNA sequence then needs to be incorporated into a host cell (through a process called transformation...FD-CF reactions affordable to many schools. Most importantly, experiments for FD-CF reactions can be fun and...

  8. Components of CRISPR/Cas9

    Type
    Blog Post
    ... combined with the tracrRNA forms the scaffold portion of a gRNA and the “spacer region” forms the target...

  9. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...the right candidate for the job. How can you incorporate the skills developed during graduate school into...whether to stay in academia or not, it is even more important to start early. Think about your plan for the ...

  10. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ... cell membrane. Heterotrimeric G proteins are important molecular switches in signal transduction pathways...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ...

  11. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using... substrate is required for imaging, and it is important to verify that this substrate does not alter cellular...

  13. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...from the mol bio side you’re sure to have many opportunities to learn about and develop technologies that...FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a ...

  14. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...NPR, articles in magazines like Fast Company, opportunities to speak at conferences, and offers for postdoc...can fit into a researcher’s work day. It’s also important to remember that it’s not the amount of time you...

  15. Hot Plasmids - February 2022

    Type
    Blog Post
    ... provides a useful yet low-pressure teaching opportunity for lab members or students new to protein expression...induce high levels of gene activation for both reporter and endogenous genes. It is highly specific in...

  16. Clear and Accessible Writing

    Type
    Blog Post
    ...reader would glean from the image itself.  As we incorporated alt text into our work, we began to realize ..., and we’d love to hear yours as well.  More importantly, you can use these four steps for any type of...

  17. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...the target cell or transiently expressed. It’s important to note that for all the applications we discuss... plasmids can induce gene-specific silencing temporarily, long-term, and even inducibly depending on the...

  18. How Addgene Creates Content

    Type
    Blog Post
    ...conversations, conferences, and questions our scientific support staff receives – in fact, the blog originally started... post (email [email protected].) We are here to support and advance the scientific community, and we can...

  19. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...aging. For both a screen and a selection, it is important to clearly define what type of factor you are ...whole genome), they knock down gene expression temporarily. RNAi libraries are especially useful if you ...

  20. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...detection limits of about 5% mutant targets have been reported for mismatch nuclease assays (2, 6, 7). Ability...nuclease assays are completely “blind” to this very important difference (Figure 3). If you are interested...
Showing: 621 - 640 of 921 results