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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 931 results for: Por

Showing: 621 - 640 of 931 results

  2. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly!  The question   The first, and most important question, you should ask for any science communication...

  6. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... of being honest and straightforward, it’s an important part of being a “good scientific citizen” and ...Press journals, including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from...

  7. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...encodes it. That DNA sequence then needs to be incorporated into a host cell (through a process called transformation...FD-CF reactions affordable to many schools. Most importantly, experiments for FD-CF reactions can be fun and...

  8. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...Gerald Schwank, first author of the cystic fibrosis report. “This is a big advantage over induced pluripotent...and many others. “Those off-target effects are important,” she said. “We also really need to get a handle...

  9. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...assembly. What makes it so forgiving, is the incorporation of a 3’-5’ exonuclease in the reaction mixture...assembly approaches, and opens the door for new opportunities in the field.  Gibson Assembly® is a registered...

  13. Components of CRISPR/Cas9

    Type
    Blog Post
    ... combined with the tracrRNA forms the scaffold portion of a gRNA and the “spacer region” forms the target...

  14. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...the right candidate for the job. How can you incorporate the skills developed during graduate school into...whether to stay in academia or not, it is even more important to start early. Think about your plan for the ...

  15. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...recommends that you sequence any portion of the plasmid that is important for your experiments. There are... Digest Introduction Sequence verification of important plasmid features (such as the gene/insert, fusion...sequences the plasmid to verify tags, mutations and a portion of the insert, but we do not sequence the entire...an example of a trace file from a high-quality portion of a sequencing reaction: Sequence near the beginning...unreliable. This is an example of a trace file from a portion of a sequencing reaction with high background: ...

  16. How Addgene Creates Content

    Type
    Blog Post
    ...conversations, conferences, and questions our scientific support staff receives – in fact, the blog originally started... post (email [email protected].) We are here to support and advance the scientific community, and we can...

  17. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...aging. For both a screen and a selection, it is important to clearly define what type of factor you are ...whole genome), they knock down gene expression temporarily. RNAi libraries are especially useful if you ...

  18. Hot Plasmids - February 2022

    Type
    Blog Post
    ... provides a useful yet low-pressure teaching opportunity for lab members or students new to protein expression...induce high levels of gene activation for both reporter and endogenous genes. It is highly specific in...

  19. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using... substrate is required for imaging, and it is important to verify that this substrate does not alter cellular...
Showing: 621 - 640 of 931 results