We narrowed to 660 results for: SIM;

1. Transferable Skills Guide: Time Management

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   ...below): Read new Zhang Lab Paper Find plasmids similar to those in the paper Ask Mary G. about her thoughts...

2. Quick Guide to Near-Infrared Fluorescent Proteins

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   ...). Modern state-of-the-art NIR FPs can be used similar to GFP-like probes by supplying a single gene to...

3. Harnessing Bacterial Toxins for Allelic Exchange

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   ...system with lambda red recombineering enables simplified chromosomal gene replacement in Escherichia coli...

4. March for Science

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   ...comparison to 37% of the general public (5, 6). Similarly 90% of active research scientists believe climate...

5. CRISPR 101: Homology Directed Repair

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   ...vertically at the orange arrows). If resolved dissimilarly (e.g. one junction is resolved on the crossing...

6. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   ... is distributed across multiple plasmids. For simplicity, we’ll use the second-generation system as an...

7. Hot Plasmids: Summer 2024

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   ... damage or permanently alter a gene product; it simply turns the target protein expression off. Plus, ...

8. An Integrin Antibody Toolkit from IPI

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   ...integrin subtype, even when in competition with other similar integrins, as reported by IPI and the Springer ...

9. Multiple Plasmids at a Low Price: Kits from Addgene

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   ...The Gomis-Ruth lab designed the pCri system to simplify the protein purification process by making it ...

10. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    ...pathway. Accomplishing this usually involves simultaneously introducing a homologous DNA repair template...

11. Technologies Enabled by NanoLuc® Luciferase

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    ...brightness and improved maturation time compared to similar orange fluorescent proteins. In their work, the...

12. Plasmids 101: Dimers and Multimers

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    ...control when performing a diagnostic digest is a simple but effective method for detecting plasmid multimers...

13. Is this the right place for me? 8 tactics for choosing a lab

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    ...emotions and assumptions. I’m not just talking about simple things like “Which route should I take to get to...

14. Plasmids 101: Restriction Cloning

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    ...grow overnight cultures for DNA purification. The simplest purification you can do is a miniprep, but if ...

15. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    ...). Mycoplasmas are considered to be one of the simplest and smallest bacteria. The absence of a rigid ...

16. When Fidelity Matters: A frank discussion about ligase fidelity

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    ...extended to multiplexed probe sets that allow the simultaneous interrogation of multiple potential SNP sites...

17. Transferable Skills Guide: Teamwork

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    ...of the phase of my life that had me listening to Simon & Garfunkel’s “I Am A Rock” on repeat in my bedroom...

18. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    ...mismatches. To use this tool to detect a BRAF gene edit, simply PCR-amplify the BRAF locus from the edited cells...

19. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    ...system once recombinant clones are generated.  A simple way to do this is to express lambda red genes from...

20. Antibodies 101: Antibody Engineering and Directed Evolution

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    ...Degenerate primers Using degenerate primers is similar to error-prone PCR. Degenerate primers are a group...