We narrowed to 730 results for: SON

...series of plasmids to study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or...

...the market between 1997 and 2000. There are many reasons why drugs fail - sex is one of them. Why might ...

...fluorescent proteins in empty backbones in the Michael Davidson Collection Find Photoswitchable Fluorescent Proteins...

...loci Building on the work of Chen et al., Thoru Pederson’s lab used CRISPR to label multiple loci in distinct...

...off-target sites from being cut. Slaymaker et al reasoned that, if they decreased the positive charge in...

...is a genome engineering powerhouse, and for good reason. It is guided by an RNA molecule, the gRNA, to ...

...scientific communication needs. The most often-cited reasons researchers might not post a preprint are reluctance...

...  Rachel Leeson...

...will still recognize your protein. For the same reason, they’re also useful in experiments where the availability...

... the brain.  References Bedbrook CN, Yang KK, Robinson JE, Mackey ED, Gradinaru V, Arnold FH (2019) Machine...

...purification without a kit that outlined several reasons why doing something without a kit has advantages...

...you are going to adopt (i.e. restriction cloning, Gibson Assembly, Golden Gate etc.), and try to find the...

...career counselor, Director of Education for the Jackson Laboratory for Genomic Medicine and a Senior Research...

...to see if it leads you to the same conclusion. Personally, I like papers that can end with some sort of...

...within E. coli host cells. Image from Nielson and Voigt (2014).   We talked with Voigt about...

...recombinase of bacteriophage P1. Sauer, B. and Henderson, N. 1988. PubMed PMID: 2839833. PubMed Central...

...writer a chance to delegate funds to supplies, personnel, equipment, travel, etc. As a first time budget...

...Though rarer, you can find MoClo that relies on Gibson and other non-Golden Gate cloning methods. MoClo...

...disease and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic...

...susceptible to artifacts and pH interference, and comparison between experiments can be more difficult due...