Addgene

We narrowed to 729 results for: Sis

Showing: 621 - 640 of 729 results

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Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Type

Blog Post

...phytochrome-like fusion proteins from the plant Arabidopsis thaliana. In the presence of red light (650 nm...
It’s Bioelectric! An Exciting Interdisciplinary Field of Research

Type

Blog Post

...encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (2016): 19575. PubMed PMID...
Antibodies 101: Selecting the Right Antibody

Type

Blog Post

...papers you could get your hands on, formulated a hypothesis, and planned out your key experiments. Unsurprisingly...
Antibodies 101: Secondary Antibodies

Type

Blog Post

... assays do not allow for absolute quantitative analysis of a sample. Instead, relative quantification ...
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

Type

Blog Post

..., nanobodies, which occur naturally in camels, consist of a single heavy chain, and they are small and...
The Future of Research Symposium Boston 2015

Type

Blog Post

...foundation for postdoc-related data collection and analysis. He is also a strong supporter of science Master...
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

Type

Blog Post

...Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry...
CRISPR Library Amplification

Type

Protocol

... on incremental, ordered cell lysis. E. coli cells are subject to lysis by freeze-thaw if not suspended...sufficient DNA for use, pool samples, and continue with analysis. “Sufficient” DNA will vary upon intended use....
Design Tips for Prime Editing

Type

Blog Post

...a prime editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer ...
Plasmids 101: Modular Cloning

Type

Blog Post

...plant biology to achieve traits such as disease resistance. MoClo is a great tool to deliver many combinations...
Progress Towards a PAM-Free CRISPR

Type

Blog Post

...xCas9 was generated by the Liu lab through phage-assisted evolution, specifically with the goal of generating...
Viral Vectors 101: Calcium Sensors

Type

Blog Post

...integration within the target cells, ensuring more consistent and reproducible results in calcium assays. There...
Viral Vectors 101: Virus Safety

Type

Blog Post

...ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic...
Fluorescent Proteins 101: Introduction to FRET

Type

Blog Post

...contribute to our understanding of diseases and even assist in drug discovery. References 1. Baird, Geoffrey...
Educational Planarian Tools for Teaching Developmental Biology Techniques

Type

Blog Post

...PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic Biology with ...
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Type

Blog Post

... codon-optimized cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes...
Mouse Modeling, Part 1: Genetically Engineered Mice

Type

Blog Post

...positive selection marker such as an antibiotic resistance gene is also included to select for successfully...
A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Type

Blog Post

...goal to develop low cost and quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020). To...
Hot Plasmids and Viral Preps - January 2021

Type

Blog Post

...Shah's and Jim Well's lab induces cre-dependent apoptosis. Now available as a ready to use viral prep in...
Synthetic promoter AAVs for cell-type specific expression in retinal cells

Type

Blog Post

...: Create promoters with the help of epigenetic analysis and designed using stretches of DNA that were ...

Showing: 621 - 640 of 729 results

Results per page:

Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

It’s Bioelectric! An Exciting Interdisciplinary Field of Research

Antibodies 101: Selecting the Right Antibody

Antibodies 101: Secondary Antibodies

Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

The Future of Research Symposium Boston 2015

Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

CRISPR Library Amplification

Design Tips for Prime Editing

Plasmids 101: Modular Cloning

Progress Towards a PAM-Free CRISPR

Viral Vectors 101: Calcium Sensors

Viral Vectors 101: Virus Safety

Fluorescent Proteins 101: Introduction to FRET

Educational Planarian Tools for Teaching Developmental Biology Techniques

Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Mouse Modeling, Part 1: Genetically Engineered Mice

A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Hot Plasmids and Viral Preps - January 2021

Synthetic promoter AAVs for cell-type specific expression in retinal cells

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