We narrowed to 731 results for: Sis;
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TypeBlog Post...salary information as of December 1st. Salary inconsistencies across geographical regions When we originally...
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Antibodies 101: Selecting the Right Antibody
TypeBlog Post...papers you could get your hands on, formulated a hypothesis, and planned out your key experiments. Unsurprisingly... -
Antibodies 101: Secondary Antibodies
TypeBlog Post... assays do not allow for absolute quantitative analysis of a sample. Instead, relative quantification ... -
Antibodies 101: Conjugation
TypeBlog Post...you can often remove the incompatible agents by dialysis or gel filtration. There is, of course, a lot... -
Plasmids 101: The protein expression toolbox
TypeBlog Post...removed. Degron tag systems include small molecule assisted shut off (SMASh), auxin-inducible degron (AID)... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...phytochrome-like fusion proteins from the plant Arabidopsis thaliana. In the presence of red light (650 nm... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post..., nanobodies, which occur naturally in camels, consist of a single heavy chain, and they are small and... -
The Future of Research Symposium Boston 2015
TypeBlog Post...foundation for postdoc-related data collection and analysis. He is also a strong supporter of science Master... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry... -
Engaging with science and society at pgEd
TypeBlog Post...rapidly changing landscape of genetics and our consistent stream of events mean that there is never a dull... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic Biology with ... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post... codon-optimized cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post...positive selection marker such as an antibiotic resistance gene is also included to select for successfully... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...contribute to our understanding of diseases and even assist in drug discovery. References 1. Baird, Geoffrey... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...Xiong Z, Yang J, Xu Z, Huang Z (2017) Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...: Create promoters with the help of epigenetic analysis and designed using stretches of DNA that were ... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...backbones expressing blasticidin or puromycin resistance genes. It’s that simple! Good luck and happy... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...goal to develop low cost and quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020). To... -
Plasmids 101: Modular Cloning
TypeBlog Post...plant biology to achieve traits such as disease resistance. MoClo is a great tool to deliver many combinations... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...xCas9 was generated by the Liu lab through phage-assisted evolution, specifically with the goal of generating...