We narrowed to 939 results for: TIM

1. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

   Type

   Blog Post

   ...compete against each other. Getting two-color stimulation has been a holy grail of the field. The problem...to try these optogenetic tools out for the first time? Boyden: There are always considerations for each...

2. "Build Your Plasmid": The Game - Play to Teach

   Type

   Blog Post

   ...playing with some cards and I had a quick flashback to time spent playing a card game called “Magic”. There ...certain target cell population. Who is the winner? Ultimately, society should be! This is a collaborative game...

3. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

   Type

   Blog Post

   ...scientists of all areas who volunteered to donate their time and expertise to help them. In less than two weeks...and easily credited. And nothing stops these one-time favors from becoming starting points for long-lasting...

4. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

   Type

   Blog Post

   ...understanding of PZA action and could potentially stimulate the development of useful diagnostic methods and...non-linear microscopy methods will be used to stimulate and manipulate neural populations in order to ...

5. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

   Type

   Blog Post

   ...reproducibility because mistakes in this can lead to wasted time or retractions. Using a catalog number for identifying...are verified and identifiable to save researchers time and make their work more reproducible. While Addgene...

6. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

   Type

   Blog Post

   ...voltage indicators (GEVIs) change fluorescence in real time when neurons or muscle cells transmit electrical... and conditions that can be used to monitor real time voltage changes in worms and have made these constructs...

7. New and Upcoming Viral Vectors - September 2019

   Type

   Blog Post

   ... four variants of these biosensors, which are optimized for specific parameters: jGCaMP7s is the most...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...

8. Summer SciComm Series: A PhD in Science Communication

   Type

   Blog Post

   ...body and how it works. I used to stay up past my bedtime, poring over my grandparents’ medical textbooks...communication as a PhD student Fortunately, by the time I was ready to graduate with my BSc in Anatomy, ...

9. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   ...transgenic flies to study your favorite gene (YFG).  Sometimes, you want to use the Gal4/UAS system, but the ... as the intestines, can be dissected at various times throughout the lifespan of the fly to determine ...

10. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type

    Blog Post

    ...perform under your experimental conditions. This is time well spent and, if you do it right, can be (part...is calculated by multiplying EC by QY (EC*QY, sometimes normalized to the value of EGFP). The higher the...

11. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    ... of Cas9 expression between experiments and over time (as compared to separate transductions with a single-vector...(Gi)-mCherry When using this virus for the first time, the Roth Lab recommends verifying cre expression...

12. Communicating Your Science With Help From ComSciCon

    Type

    Blog Post

    ... and develop skill in science communication over time.  Like most skills, great science communication ... to pose these why questions to yourself, but ultimately there is no substitute for feedback from others...

13. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    ...How many times have you looked at a diagram depicting transcription, or DNA repair, or replication, or... Berkley Gryder. Addgene depositors or not, your time is valuable ...

14. A Novice's Perspective on Neuroscience - SfN2017

    Type

    Blog Post

    ...a talk about the different parts of the brain stimulated by stories. In retrospect, I should have expected...looking to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene Blog...

15. CasPEDIA: A Functional Classification of Cas Enzymes

    Type

    Blog Post

    ...Once upon a time, not so long ago, spCas9 was the only Cas enzyme widely available and applied by researchers...better experimental fit due to this. But now, it’s time to cast aside those concerns and embrace a new resource...

16. New and Upcoming Viral Vectors - May 2020

    Type

    Blog Post

    ...targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

17. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type

    Blog Post

    ...changes simultaneously with 54% efficiency, about 7 times higher than ABE7.10. However, the increased editing...the ABE8e plasmids at Addgene! Why engineer and optimize base editors? Targeted evolution of naturally ...

18. Antibodies 101: Monoclonal Antibodies

    Type

    Blog Post

    ...acid sequence of the antibody-encoding genes over time as the hybridoma cells divide. These changes can...meaning that there will be changes between lots over time. Specifically, the antigen-binding sites of the ...

19. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type

    Blog Post

    ...bogged down with distributing them and spending more time on research. What do you hope Addgene will accomplish...and participates locally in Read To A Child's "Lunchtime Reading Program" in Cambridge, MA. Additional...

20. Behind the scenes: Addgene’s new search engine and more

    Type

    Blog Post

    ...catalog are very well-characterized. During this time, we’ve also created a variety of educational resources...the foundations for many improvements to come. Ultimately, we would like our search engine to be so specialized...