We narrowed to 1,082 results for: TOR;
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TypeBlog Post...improve its stability. Additionally, the choice of vector can impact the expression level of an antibody ...lesional skin from a psoriasis patient, showing an inflammatory phenotype. Each protein was detected using an... cellular pathways by binding to cell surface receptors. These applications are typically used for investigating...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...
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Trends in CRISPR and SynBio Technologies
TypeBlog Post...fields. Addgene is a global nonprofit plasmid repository. Over 2,000 labs have deposited plasmids to Addgene...Zhang acknowledge the role of data and materials repositories in aiding the dramatic spread of the Cas9 technology...accelerated through a combination of open-source distributors such as Addgene, as well as a number of online... -
Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew
TypeBlog Post...new primary investigator. Coming up with new project ideas, applying for grants, and mentoring. Chapter ... Chew! Wei Leong Chew is a Junior Principal Investigator at the Genome Institute of Singapore. His lab... -
Selecting Your Plasmid Purification Kit
TypeBlog Post...any applications. DNA quantity is fairly self explanatory and achieved by performing different sized preps...about!) are a significant source of waste in laboratories. To help combat this, some manufacturers have...Minimized amount of silica ACT environmental impact factor label Non-silica based, avoids use of chaotropic...kit for your lab is dependent on many different factors as we detailed above, so be sure to pick what kit... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog Post... fans of all our depositors here at Addgene — your contributions make the repository what it is. So we...we’re thrilled this week to congratulate depositor David Baker, who was awarded the Nobel Prize in Chemistry... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog Post...2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9 and other....G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000... our Viral Production Process Find other Viral Vectors ... -
Tips for Working in Industry & Success in Management: Interview with Crystal Shih
TypeBlog Post...Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland...'ve helped her at Novartis. The importance of mentorship in developing career skills. Outro Many thanks...Addgene Find more management resources in our Mentoring for Scientists Series Learn about Turning Your... -
Addgene Stands with the Scientific Community
TypeBlog Post...resources: Preserving scientific assets: We hold a repository of valuable research materials that might otherwise...continue to offer high-quality DNA sequencing, viral vector preparations, and antibody production that labs...continue. Moving Forward Together Throughout history, the scientific community has demonstrated remarkable... -
A Scientist's Guide to Ebola
TypeBlog Post...the virus itself that kills you, but the cytokine storm it ultimately sets off. The virus works its way...cells and turn the cell into a veritable virus factory. Their findings were reported in the Journal of...and the target of neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading... -
Addgene's Website Update
TypeBlog Post...two options: “Search Repository” and “Deposit Materials”. The “Search Repository” function is our same...section: Tools of the Day. Our plasmid and viral vector of the day have a fresh new look and a fresh new... -
How Do I Place an Addgene Order Using a PO Number?
TypeBlog Post...'Regan Ordering jeans online from your favorite store is easy. Ordering genes on plasmids online is a ...nonprofit, Addgene does not own the material in our repository. We distribute it on behalf of depositing institutions...full of information about how to deposit plasmids, store your plasmids or viral items, technical support,... -
Our Most Popular Blog Posts from 2019
TypeBlog Post...Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley,...Academic Job Interview by Erik Snapp Addgene depositor and guest blogger Erik Snapp breaks down how to... blog posts Resources at Addgene Browse the repository Find educational resources at Addgene Search ... -
Top 10 Open Science Developments of 2013
TypeBlog Post..., even when all kinds of other factors, including journal impact factor, study topic, and the corresponding...preference to journal access granted via a public repository or authors’ own web sites. 9) The year 2013... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog Post...MCF10A cellular models. Ba/F3 and MCF10A are growth factor dependent cell lines and any mutations that that...advantages will be revealed in the absence of growth factor. The team assigned the mutations to functional ...significantly increased cell viability, and these “activators” may be the direct drivers of tumor formation... -
9 Tips to Achieve Success in Academia
TypeBlog Post..., and young Principal Investigators (PIs). We've interviewed Addgene depositors who are at various stages...different ways to succeed – as loner or gregarious; dictator or laissez-faire; theory or practice; business-like... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post...neurons that trigger the CPG (central pattern generator) (Wyart et al., 2009). The CPG is a neural circuit... involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...optogenetics blog posts Read blog posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...biodistribution therefore introduce additional confounding factors when attempting to interpret signals from these...collapse E. coli re-express arg1 gas vesicles and restore their ultrasound signal. Using acoustic reporter...years to clinically treat enteric infection and inflammatory bowel conditions. In these experiments, arg1... -
An Addgene Summer Internship
TypeBlog Post... gene in a pLenti vector backbone. During those five weeks, I worked with my mentors, Katherine, Amrita...exchanged contact with Amy C., Addgene’s intern coordinator shortly after hearing her talk about Addgene’... -
Build Your CRISPR Vocabulary
TypeBlog Post...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...array: The section of the bacterial chromosome that stores a genetic record of previously encountered foreign...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ... -
We're Updating Our Hold for Publication Policy
TypeBlog Post...original policy was straightforward, but cumbersome: depositors had to email us with permission to release their... are left in the hold status and reach out to depositors about them, but we all know how easy it is to...your work. Plus, it’s costly for us to process and store materials that aren’t distributed or for our teams...