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  1. 6 Tips for Grant Writing

    Type
    Blog Post
    ...paper, but positive outcomes can include poster presentations, talks about your work, or even the successful...Learn How to Become a Grant Writing Professional Facilitate Career Planning and Personal Development with...

  2. Tips for Technical Support Calls

    Type
    Blog Post
    ...scientific articles, patents, and even some doctoral dissertations. Streamline the question asking process Having...answer, consider asking to avoid unrealistic expectations. And finally, provide feedback. If you are...

  3. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all the original...deleting a problematic copy of a gene that causes a debilitating disease! This is, of course, only hypothetical...

  4. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...eventually found a version of AsLOV2 with five mutations (C450P, L496V, Q513K, G528K, D540M) that shows... and ΔF/F of MagLOV, a variant of AsLOV2 with mutations C450P, L496V, Q513K, G528K, D540M, in response...

  5. Of Myc and Men

    Type
    Blog Post
    ...partner; as I write this, there are close to 10,000 citations for anti-Myc 9E10 in CiteAb, many of which are...no antibody is that perfect. One of the known limitations was described by Schüchner, et al., who published...

  6. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...due to its endA1 recA1 relA1 genotype, as these mutations improve plasmid stability and yield. If a plasmid...using older plates could increase plasmid loss or mutation. Let it incubate Increasing the incubation time...

  7. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...for CINSO. For instance, I tweeted to the Indiana State Museum and others when I was trying to find local...informal science educators, and ever since, the State Museum has been the best supporter and most productive...

  8. Plasmids 101: Protein Expression

    Type
    Blog Post
    ..., tobacco, rice, sugarcane, and even tubers of potatoes have been used for protein expression. Plant ...well-defined media and can be easily adapted to fermentation allowing for large-scale, stable production ...

  9. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... Base editing, another method to create point mutations, also has a strict editing window about 15 nt ...mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created a selection...

  11. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...industry, you’re more likely to give an oral presentation to highlight any new data or ask for additional...industry with most team members giving oral presentations, you will also have to attend many more meetings...

  12. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... that was not “poised for cleavage”. Jacob computationally added the non-template strand to the Jinek ... this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce...

  13. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...reflected light by using a pinhole.  However, the excitation light excites all of the fluorophores as it passes...takes to image through a large z-stack (8).  One limitation of LSFMs using single side illumination, especially...

  14. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...BLAST (blastx) search to check for expected ORFs, mutations or truncations. A primary advantage of blastx ...database. Another benefit is that a frameshift mutation present in the ORF is readily apparent when viewing...

  16. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13(7), 1288. https://... the National Academy of Sciences of the United States of America, 54(1), 137–144. https://doi.org/10.1073...

  17. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...Fixation allows you to freeze your samples in a given state or point in time. You want to halt degradative processes...methanol, or ethanol, work by dehydrating and precipitating cellular components. This method of fixation...

  19. CRISPR Antimicrobials

    Type
    Blog Post
    ...the world is unprepared. Each year in the United States, two million people will be infected by antibiotic...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization...
Showing: 621 - 640 of 836 results