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  1. Resume Writing for Non-academic Science Careers

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    Blog Post
    ...you did. Emphasize the skills. If you studied astronomy or bioinformatics data, then the chance is that...Communications Coordinator for the Department of Physics & Astronomy, University of British Columbia. Originally on...

  3. Advice for Scientists Starting a Lab

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    Blog Post
    ...equipment always helps. But how does one build a strong collaborative research team motivated by similar...selectively picking the first couple of hires to build a strong, collaborative team. And take advantage of the ...

  4. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post
    ...base pairing between these ~4 base overhangs isn’t strong enough to hold a plasmid together through transformation... reading Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge...

  5. A Deep Dive into BioBuilder

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    Blog Post
    ...extracts to perform transcription and translation in vitro, and the new BioBuilder module allows students to...Assembly Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio Plasmids ...

  6. Communicating Your Science With Help From ComSciCon

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    Blog Post
    ...already grown out of our workshops. If you're having trouble finding science communication opportunities in ...of its Leadership Team.  Nathan did his PhD in astronomy and works as a data scientist in the entertainment...

  8. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post
    ...Please keep in mind that this is just a brief introduction to the wide, wild, and fascinating field of ...Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies Resources on Addgene.org ...

  10. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post
    ..., you’ll want the proper positive and negative controls for your PCR.  Find the details and learn more... this blog post for tips for analyzing and troubleshooting Sanger sequencing results. At Addgene, we now...

  11. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post
    ... Image from Wen et al., 2017. Once you’ve introduced the reporter into the cells, you’re ready to ...sequence is also found in the reporter gene). A control RFP expression plasmid.  With this set up, all...

  14. Screening for Successful Genome Editing with Digital PCR

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    Blog Post
    ...minimally informative. In this blog post, we’ll introduce digital PCR as an emerging validation technology...assays for mutation screening Now that we have introduced how digital PCR works, let’s explain what kind...

  15. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ...team develops methods, performs extensive quality control, generates  educational materials, and provides...details about virus production protocols and quality control can be found on Addgene’s Viral Production webpage...

  16. Plasmids 101: Blue-white Screening

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    Blog Post
    ...operon.   Tips for blue-white screening Use a good control: Transform the backbone plasmid without insert....reproduce to form colonies (used to complement auxotrophic cell lines), or genes which rescue a lethal phenotype...

  17. 3 Challenges in Plant Synthetic Biology

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    Blog Post
    ...other than to sustain those techniques. We feel strongly that the scientific and the agricultural community...aesthetics with this technology and giving consumers control over their interaction with it, we hope to inspire...

  18. Antibodies 101: Fab Fragments

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    Blog Post
    ... means that F(ab’)2 fragments may have overall stronger interaction with their antigens than a Fab or ....2022.100301  Additional resources on the Addgene blog: Introduction to AntibodiesSingle Chain Fragment Variables...

  19. To Each HIS Own

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    Blog Post
    ...the lab (for a refresher see Antibodies 101: Introduction to Antibodies.) Unfortunately, for some proteins...Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to Antibodies References Hochuli E, Bannwarth...
Showing: 621 - 640 of 914 results