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We narrowed to 750 results for: Uty

Showing: 621 - 640 of 750 results

  9. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...effector nucleases. These plasmids were designed to utilize the BsaI and BsmBI Type IIS sites such that custom...

  11. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...swirling the flask occasionally as the solution heats up.). Caution HOT! Be careful stirring, eruptive boiling...do you get better resolution of bands? A few simple ways to increase the resolution (crispness) of your...gel electrophoresis, including tips to improve resolution and separation of bands. Protocols... completely dissolved (but do not overboil the solution, as some of the buffer will evaporate and thus...continue towards a boil. Keep an eye on it the solution has a tendancy to boil over. Placing saran wrap...necessary if you pay close attention. Let agarose solution cool down to about 50 °C (about when you can comfortably...0.2-0.5 μg/mL (usually about 2-3 μl of lab stock solution per 100 mL gel). EtBr binds to the DNA and allows...

  12. Immunocytochemistry

    Type
    Protocol
    ...working solution: Prepare a 300 µM DAPI stock solution by diluting 2.1 µL of the 5 mg/mL DAPI solution to ... 300 nM DAPI working solution by diluting 5 µL of the 300 µM DAPI stock solution into 5 mL PBS. Protect... and 30 µL Triton X-100 in 10 mL PBS. Antibody dilution buffer: Dilute 0.5 g BSA and 150 µL Triton X-100...Remove the paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of...antibody to the desired concentration in antibody dilution buffer. Pro-Tip The optimal antibody concentration...Counterstain nuclei with 500 µL of 300 nM DAPI working solution for 10 min at RT in the dark. Remove the DAPI ...

  13. AAV Production in HEK293 Cells

    Type
    Protocol
    ...hydrochloric acid until the solution clears. Check the pH of the solution and use hydrochloric acid or... 1 g/L glucose. 1 mg/mL polyethylenimine (PEI) solution: Dissolve 100 mg of PEI powder into 100 mL of ...to adjust the pH to 7.0. Pro-Tip The pH of this solution will drift pretty rapidly upon addition of acid...over or undershooting the desired pH. Allow the solution to mix for 10 min and then recheck the pH to ensure... ensure that it has not drifted. Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into... Store the tubes at -80 °C. After thawing, the solution can be stored at 4 °C for up to 2 months. After...working stock. 40% POLYETHYLENE GLYCOL (PEG) 8000 solution: Dissolve 400 g of PEG 8000 and 24 g of NaCl ...

  15. Engaging with science and society at pgEd

    Type
    Blog Post
    ...organizations, libraries, youth groups, and faith institutions. The conversations often move quickly into sensitive...practical and ethical level, scientists arguably have a duty to engage the public on the implications of their...

  17. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult dentate...

  18. Design Tips for Prime Editing

    Type
    Blog Post
    ... newly synthesized strand before heteroduplex resolution, which can lead to indels (Anzalone et al., 2019...
Showing: 621 - 640 of 750 results