... post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ready-to-use...next time you test an antibody (perhaps one from Addgene!), consider sharing your results so that others...References and Resources Additional resources on the Addgene blog:  Still trying to choose an antibody? Check...-to-use antibodies from Addgene Designing a recombinant validation experiment? Find plasmids here!  References...

...an instructional text file. What's available at Addgene? In total, we constructed and tested eight two-...in the table below and can be requested through Addgene. Their plasmid maps and GenBank files can be found...the plasmid maps and GenBank files uploaded to Addgene. We also recommend inserting self-cleaving hammerhead...PMCID: PMC4245323. Additional Resources on the Addgene Blog Learn How Piggy Bac Vectors Can Be Used for...of Our SynBio Posts Additional Resources on Addgene.org Visit Our SynBio Pages Check Out More Lu Lab...

...Immunofluorescence Resources on Addgene.org Find recombinant antibodies available at Addgene Protocols for Immunocytochemistry...IHC validated or ICC validated antibodies from Addgene), or be prepared to run some extra controls and...three targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed...10.1073/pnas.2018488117 Additional Resources on the Addgene blog Introduction to Antibodies Which Fluorescence...

...targeting guide here! Additional resources on the Addgene blog Download the viral vectors 101 eBook Read...viral service Find retrograde AAVs at Addgene ...Learn more about retrograde AAVs Resources on Addgene.org Read our AAV guide Learn more about our viral...

...Additional resources on Addgene.org Find molecular biology protocols here Visit the Addgene careers page ... Capsid and Tail. Additional resources on the Addgene blog Read our blog posts on open science Find ...

...that expound on bench skills and experience. Most Addgene PhD level job descriptions clearly describe that....  You might also enjoy the other posts in the Addgene Blog Scientist Careers Series.    ...

... complete collection of protocol videos on the Addgene YouTube page. ...

...interest; more recently we have also been using Addgene CRISPR vectors to generate cell lines with knockouts...figure 1.   Find Additional Useful Protocols on Addgene's Protocol Pages Agarose cloning method procedure...som.umaryland.edu.   Additional Resources on the Addgene Blog Tips for a 1st Time CRISPR User (by a 1st...Plasmids 101: Mammalian Vectors Resources on Addgene.org Check Out Our Protocols Pages Browse Fluorescent...

...own mutant flies. Find Drosophila Resources at Addgene The birds and the bees when it comes to fruit ...basic knowledge to start working with flies. Like Addgene, the Drosophila community is highly sharing-oriented... a “pound symbol”. Additional Resources on the Addgene Blog Methods for Site Specific Recombination in...Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources...

...Antibodies Resources on Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant...the-process-of-fixation-and-the-nature-of-fixatives/  Additional resources on the Addgene blog: Antibodies 101: Introduction to Immunofluorescence...recombinant antibodies at Addgene  ...

...counter-selection Resources on Addgene.org Find plasmids at Addgene Read our molecular biology reference...plasmid cloning. Additional resources on the Addgene blog Read our Plasmids 101 blog posts Learn more...

...scientistswhotweet. And don’t forget to follow us @Addgene.   References Ke, Q., Ahn, Y., & Sugimoto, C....scientist? Check out this post. Resources on the Addgene Blog 6 Tools & Tips: Online Social Networking ...

... OR enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration...fluorescent molecule   Additional Resources on the Addgene Blog Check out the Rosella Autophagy Sensor Read...Addgenie Mary Gearing contributed to the content of this article. Biosensors (‘biological sensors’) are...CRISPRainbow for Genome Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids...

...protein-by-protein basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames,” it...PMCID: PMC4252608. Additional Resources on the Addgene Blog Sign Up to Receive the Fluorescent Protein...about Small Molecule Fluorphores Resources on Addgene.org Find Fluorescent Protein Empy Backbones Visit...

...research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau, R.W., Lee-Gosselin, A....PubMed PMID: 29300010. Additional Resources on the Addgene Blog Learn more about in vivo imaging tools The...fluorescent protein reporter gene Resources on Addgene.org Find plasmids for in vivo imaging with fluorescent...

...new X-CHIME systems, developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment....1038/s41467-019-09656-2 More resources on the Addgene blog CRISPR101: Validating Your Genome Edit How...Genome Editing CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions...

.../10.1073/pnas.2018488117 More resources on the Addgene blog Antibodies 101: Multiplex Immunofluorescence...Immunofluorescence Negative Can Be Positive: Open AAV Data with Addgene Antibodies 101: Choosing the Right Isotype...

...Data Sharing Resources at Addgene.org Deposit Your Plasmids with Addgene Find Molecular Biology Protocols...innovation by all.  Additional Resources on the Addgene Blog Use Apps to Save Time and Track Your Experiments...

...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...Editing the Epigenome (plus bonus podcast with Addgene Advisory Board member Alex Chavez) Day 11: Pooled...

...Principal Investigators (PIs). We've interviewed Addgene depositors who are at various stages in their careers...journalism, entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped...