Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
Type
Blog Post
...line), armed only with viruses obtained through Addgene’s viral service and the methods sections of scientific...of performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools.
Schematic overview...repeatability and bad news for a screen. Because Addgene viruses are provided at a specific titer, you can...Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609.
Addgene Materials Used
1. lentiCas9-Blast (Plasmid #52962...PMCID: PMC4744125.
Additional Resources on the Addgene Blog
Genome-wide Screening Using CRISPR/Cas9
Deliver...Mouse Models Using CRISPR/Cas9
Resources at Addgene.org
Generating Stable Cell Lines with Lentivirus...