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We narrowed to 813 results for: cell

Showing: 621 - 640 of 813 results
  1. Sharing is Caring: But How to Distribute Open Hardware?

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    ...quantitatively evaluating antibody staining, or counting cells are now routine, open-source software projects that...portable device for studying plankton using flow cells, has become an established alternative to high-priced...
  2. Plasmids 101: The protein expression toolbox

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    Blog Post
    ...force to control protein expression in mammalian cells: easy to use biological tools are abundant! In this...tag prior to introduction of the construct into cells. However, if you would like tunable control of an...
  3. Viral Vectors 101: Inverted Terminal Repeats

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    ...allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important...Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum Gene Ther Methods, 28(5), 277–289. https...
  4. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post
    ...strain, the promoter being used should be tested in cell lines to determine how it impacts the expression...interest. Cre may be expressed globally (in every cell of the mouse) or in a tissue-specific manner (with...
  5. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    Blog Post
    ...annealing to a complementary ssDNA target in the cell.  Only Beta expression is required for recombineering...definitely the easier of the two options, but these cells are prone to mutations and will have more unintended.../Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering ...
  6. The Materials Science of Optogenetics Experiments

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    ...Pharmacology and Optogenetics. Cell 162.3 (2015): 662-674. doi: 10.1016/j.cell.2015.06.058. Pubmed PMID: 26189679...protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological chemistry. 2011;286(29...Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular Biology from the University of Michigan...PMCID: PMC3466483. 4. Kim TI, et al. Injectable, cellular-scale optoelectronics with applications for wireless...
  7. Common Injection Routes in Mice

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    ...different substances can be injected, including cells, drugs, DNA, viral vectors, and viruses. All researchers... injections are most often used when injecting a cell suspension to create xenograft models. Restraint...
  8. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ...screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol...non-coding RNAs with the CRISPR/Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi...encode proteins. They play important roles in many cellular and regulatory processes, but much of their functionality...
  9. Call for Guest Bloggers

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    ...Your Favorite Fluorescent Protein Oligomerizes in Cells   ...and many more! Sign up to write for the Addgene...
  10. Course-Based Undergraduate Research Experiences (CUREs)

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    ...identifying new genes required for stem and progenitor cell maintenance in planarians in Developmental Biology...junction complex is required for stem and progenitor cell maintenance in planarians. Developmental Biology... his teaching style, with the former offering excellent training in improvisation and the latter making...
  11. How to Write a Scientific Review Article

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    Blog Post
    ...a review article on histone modifers in B and T cells with my adviser when I was a graduate student. Naturally...acetyltransferases and histone deacetylases in B-and T-cell development, physiology and malignancy." Genes &...
  12. Tips for arabidopsis transformation

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    Blog Post
    ...incubated at stationary phase for too long, the cells in your culture will no longer be viable. The time...graduate student at Stanford University. She studies cell fate maintenance in the stomatal lineage and has...
Showing: 621 - 640 of 813 results