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We narrowed to 986 results for: des

Showing: 621 - 640 of 986 results
  1. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...come. Some Freshers Fairs can include fairground rides and other fun activities and even invite members...its members to become involved with. The society provides talks and career support for a range of different...
  2. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...transposon system is one such system which has been designed to perform gene transfer in vertebrates. The system...whose use is only likely to grow in the coming decades.  References 1. Kowarz E, et al. Optimized Sleeping...
  3. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...the measure of work is not just time spent at the desk, but it is worth it. This requires a focus on deliverables...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...
  4. Advice for Choosing a Research Project

    Type
    Blog Post
    ...good and bad data, and knowing how to follow the design, execute, analyze, repeat pathway that is required...critically before diving in. Research projects are designed to train graduate students and postdocs to think...
  5. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...when the cells are grown on selective media, and provides those bacteria with a pressure to keep your plasmid...gene and acts as a marker for the mutation. When designing these types of experiments it is best practice...
  6. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...PDF at the end of this blog post. This document includes a list of questions to ask your potential advisors...some good pre-work you can find a lab that will deserve your hard work and an advisor that will be a lifelong...
  7. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...without end-point sequencing assays.  This tool was designed by putting together the coding sequence of a mCherry...MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent...
  8. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...the Addgene website. blog.addgene.org The blog provides tips for working with viruses including advice...on each viral prep’s specific material page and includes western blots as well as other expression data...
  9. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies...
  10. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...if you end up concentrating your AAV above your desired titer and want to dilute it, err on the side of...less buffer than you would to achieve the exact desired concentration to avoid over-diluting, and make ...
  11. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually...
  12. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...visiting role is because the host lab or institute provides something that you can’t obtain at your current...skills and equipment! Preparing for your visit   Design and communicate a research plan Your time at the...
  13. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...biomass production techniques in the following decades, Phillips made the decision to release the K. phaffii...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...
  14. Hot Plasmids - January 2023

    Type
    Blog Post
    ...effects, both of happen with Cas13. CRISPR-Csm degrades both nuclear and cytoplasmic mRNA with 90-99% ...crystalizing the hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you...
Showing: 621 - 640 of 986 results