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TypeBlog Post...developments. These abrupt changes threaten to slow transformative biological discoveries and life-saving medical...access to high-quality scientific materials and information. Our agility as a nonprofit resource can prove...
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A Scientist's Guide to Ebola
TypeBlog Post... also recently described how the glycoprotein transforms itself to escape vesicles within host cells and...offered to answer readers' questions. If you have information or another resource to add, please tell us about... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...with biorender.com Both gradients can be informative, so spend some time thinking about which type...blockers, you can run a comparison of different formulations instead of concentrations. Most labs use protein-based...rule out) blocking issues. If your antibody information doesn’t contain blocker recommendations, you ... -
What's New in CRISPR - May 2019
TypeBlog Post...Candida albicans A new CRISPR interference (CRIPSRi) platform for targeted gene repression in Candida albicans... bloggers. Check out this blog post for more information. Additional resources on the Addgene blog ... -
Download Science-Themed Zoom Backgrounds from Addgene
TypeBlog Post...px or 1080 px). Do you do a lot of transformations with the plasmids you receive from Addgene?...shipped as bacterial stabs? You can learn about what format your material will come as (and more!) on Addgene... -
Addgene's Website Update
TypeBlog Post...scroll down? You’ll find Blugene, as well as information about Addgene, our mission, links to our deposit...website easier to navigate, thanks to design choices informed by Web Content Accessibility Guidelines (WCAG)... -
Let There Be LITE Plasmids
TypeBlog Post...dynamically.” To understand how that works in memory formation or other brain functions, Konermann needed tools...For those who do want to apply LITEs for the transformation of neurons, she offers a tip: save yourself... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog Post...interests and skillset. With this information in hand, you can then formulate a plan to further develop your... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post...inversions [3]. These rearranged segments may also be informative as to genomic function. A second use of targeted... Refereces: Browse CRISPR Plasmids CRISPR Information at Addgene More CRISPR-Cas Blog Posts ... -
What's New in CRISPR - March 2020
TypeBlog Post...fluorescently labeled piggyBac vectors to deliver uniform and sustained expression of multiplexed gRNAs in... bloggers. Check out this blog post for more information. Additional resources on the Addgene blog ... -
What's New in CRISPR - September 2019
TypeBlog Post...has previously used the library with SpdCas9 to perform pooled CRISPRi screens to identify essential genes... bloggers. Check out this blog post for more information. Additional resources on the Addgene blog ... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...taking info from the code of viruses to give information to the computer to fight the known viruses. Bacteria.... As Dr. Mello said, “Cells have been in the information age for billions of years (…) Bacteria are really... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog Post...of plasmids is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent Protein...fluorescent proteins are concerned.” For more information on choosing a backbone and making fusions, see... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...scientific outreach and community engagement. Information about contacting Addgene can be found on our ...struggled to get those plasmids with the right information and I believe that a lot of European students... -
Plasmids 101: What is a plasmid?
TypeBlog Post...vector containing the newly inserted gene is transformed into bacterial cells and selectively grown on...Addgene’s Molecular Biology Reference includes information about molecular cloning, how to choose a plasmid... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post... they could solve this problem. D10A nickase outperformed both WT Cas9 sites tested by a wide margin, ...use Cas9 nickases in genome editing. For more information and a link to the archived webinar, click here... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post...that much tissue takes a week of multiple people performing dissections. With CUT&RUN, we have had success...up until this point. The nuclease reaction is performed on ice, and only for a short period of time, thus... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog Post...Some use cases include: Cataloging a reagent’s performance as in this antibody ReFigure Analyzing replications...confirmatory image to show that a previously used reagent performs well, or you might publish a replication experiment... -
Sensing Neuronal Dopamine
TypeBlog Post...fluid (Darvesh et al., 2011). This technique is performed with a probe surrounded by a semipermeable membrane...The advantage of this method is that it can be performed in a live awake animal by surgical implantation... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post...react by performing a distinct C-bend to escape possible danger (see figure). Larvae also perform spontaneous...