We narrowed to 759 results for: rest

1. Multiplex Genome Editing with CRISPR-Cpf1

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   Blog Post

   ...multi-gene editing with a single crRNA array. Are you interested in using Cpf1 multiplexing in your research? ...

2. Addgene’s Viral Service - Why Virus? Why Now?

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   Blog Post

   ... developments on this project and on all the interesting things going on at Addgene. Sharing speeds science...

3. Top Requested Lentivirus and AAV of 2016

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   ...in transgenic animals, where cre expression is restricted to a particular cell type. The human synapsin...

4. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   ...gRNA and enrich cells for your CRISPR mutant of interest, give these methods a try.  References Højland...

5. Antibodies 101: Monoclonal Antibodies

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   Blog Post

   ... binds to a specific region of your protein of interest. For example, if you are studying a protein that...

6. How to Keep a Lab Notebook for Bioinformatic Analyses

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   ...University of Pennsylvania. She is particularly interested in science communication and epigenetics. Follow...

7. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   Blog Post

   ...multifragment assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis...

8. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   ...share it as soon as possible. Addgene: How much interest are you seeing in this new tool? Mendenhall: We...

9. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   Blog Post

   ...great potential for multiplexed genome editing. Interested in using PTG plasmids in your research? Check...

10. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post

    ...off, to reversibly activate or repress genes of interest. They also “showed that different guide RNAs have...

11. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    ...biotech startup process and work environment! Interested in Synthetic Biology? Read On! Synthetic Biology...

12. Dos & Don'ts When Publishing a Scientific Manuscript

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    Blog Post

    ...whether your manuscript fits the scope and the interest of the journal. Be sure to check the Author Guidelines...

13. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    Blog Post

    ...there a senior person whose career path you are interested in following? Someone you admire for their insight...

14. Transferable Skills Guide: Public Speaking

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    Blog Post

    ..., much too late, that you labeled your gene of interest incorrectly on slides 8-16. Your notes disappear...

15. RANbodies: Reporter Nanobody Fusions

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    ...Antibodies are a go-to tool for detecting a protein of interest in cells and tissues. Although antibody production...

16. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post

    ...multiplexable RNA knockdown. Cas13 has also stirred up interest for its potential as a viral therapeutic that ...

17. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    ...These modifications result in conformationally restricted RNA which improves mismatch discrimination and...

18. Antibodies 101: Affinity Reagents

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    Blog Post

    ...chains made by immune systems. But here, we're not interested in antibodies. Instead, we're going to focus ...

19. Antibodies 101: Selecting the Right Antibody

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    Blog Post

    ... been validated in the species your protein of interest comes from. If one is not available, don’t lose...

20. What's New in CRISPR - November 2021

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    Blog Post

    ... within the FAH gene was precisely corrected, restoring expression in the liver.  Read the paper in Nature...