We narrowed to 997 results for: lars;

...experiments ranging from transient gene expression in a particular cell type to CRISPR genome editing, optogenetics... also be used to ensure specificity; this is particularly helpful when wanting to target specific neuron...vector. Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid and individually... conversion of the DNA goes through the inter-molecular annealing or second strand synthesis. In contrast...transcriptionally active double-stranded form through intra-molecular annealing. Figure and legend from Okada, 2013....Production System: Towards Gene Therapy For Duchenne Muscular Dystrophy. InTech. doi: 10.5772/53023 Veldwijk...method for the titration of AAV-2 vector stocks."Molecular Therapy 6.2 (2002): 272. PubMed PMID: 12349826...

...Antibody Validation proposed 5 pillars for antibody validation.  The 5 pillars include genetic strategies ... project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background...successfully used in conditions that are the most similar to yours. Take a look at the publications cited...application, a publication successfully used it in a similar way as you plan to. To secondary or not to secondary...technical support team, is a safer bet than one from a large distributor that may not have direct experience ...been validated using one or more of the proposed pillars.  Remember, validation does not mean that the antibody...

...Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced... can be excited many times, thereby producing a large number of emitted photons before they are destroyed...absorb the excitation light less efficiently. Similarly, decreasing the power of the excitation light ...these factors, environmental conditions such as cellular redox state and oxygen concentration may affect...Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced...Marieke Mastop is a PhD candidate at the Section of Molecular Cytology (University of Amsterdam) where she develops...Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire Fluorescent Protein...

...two are present; intramolecular events happen with greater frequency than intermolecular events, but any... kDa protein responsible for intra- and inter-molecular recombination at the loxP recognition sites. A...sequence between the loxP sites is excised as a circular piece of DNA (and is not maintained).  Translocation... is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is from Tyler Jack's lab: ...purposes, including activating an oncogene only in a particular organ, or bypassing embryonic lethality. To ...site-directed recombination technology very conceptually similar to Cre-lox, with flippase (Flp) and the short flippase...

...Common self-labeling tags Let’s take a look at some popular self-labeling tools: Tetracysteine: The first reported...variants run up to HaloTag11. HaloTag is now the most popular self-labeling tag in Addgene’s collection. CLIP-tag...on the protein of interest. Table 1: Summary of popular self-labeling tag systems Tag Size (amino... HaloTag for their "ticker tape" method for intracellular recordings, switching between different colored...Cornish, 2011). HaloTag and SNAP-tag are the most popular self-labeling enzymes in Addgene’s collection, ...that self-labeling protein tags are increasingly popular. Embrace the flexibility and switch things up for.... Two-colour live-cell nanoscale imaging of intracellular targets. Nature Communications, 7(1), Article...

...pitfalls of accepting alternative facts. Because, similar to scientists, the legacy of each government and...scientific consensus and calling for billions of dollars in cuts to important research funding.  So when...sacrifice in April because devaluing science has alarming implications for our public policies—many of which...that the community of science supporters is much larger and stronger that I expected. Jennifer Stancill...Birmingham, AL, USA 33.5207°N 86.8025°W - Jonathan Aguilar Galileo wrote, “now you would not hide behind these... at the same time pass over in silence his very large one” (http://oll.libertyfund.org/titles/galilei-...deserve to know the truth about our world. Jonathan Aguilar (@AggleDetector on Twitter) is a graduate student...

... inherited by daughter cells during cellular reproduction, similar to the host’s chromosome, or they can...both Archaea and Eukaryota can also harbor these circular DNA passengers. Most natural plasmids encode beneficial...transfer can occur naturally through transformation (cellular uptake from the environment), transduction (viral... in a minute! Fun fact! Electroporation is one popular method scientists use to promote transformation...encoding these functions form the basis of many molecular biology tools! Figure 1: Common functions... Mother Nature’s toolbox for new and exciting molecular technologies!  This post was originally written...

...to be large enough to target larger areas, typically a few hundred nl. Alternatively, for larger brain...activity, commonly via light-activated ion channels. Similarly, chemogenetic tools are designed channels that...also reduce expression levels of certain genes. A popular method uses Cre recombinase on transgenic flox ...lentiviral vectors are superior packaging vectors for larger genes - they can hold up to 8 kb, while AAV can...liquor of the cerebral ventricles (ICV, intracerebroventricular) to minimize tissue damage; however, the...

...and uses the similar buffers as would be used for a mini prep, except it does it on a larger scale. Using...for standard molecular biology techniques Browse our video library Check out our molecular biology reference...able to do high throughput DNA preps and other molecular work if we need it to. This gives us the oppotunity...the Biomek FxP is it’s customizability. It has a large deck that can house many different types of devices... DNA purification We're developing ways to prep large quantities of DNA because some countries just can...

...Biodesign Center for Molecular Design and Biomimetics and the School of Molecular Sciences at Arizona ...In response, the World Health Organization has declared a global health emergency and called for the fast-tracked...sensors within an extensive infrastructure of molecular and electronic companion technologies that has...1x106-fold lower than before), and our combined biomolecular tools can distinguish between global strains...detect the virus in a clinically relevant setting. Molecular diagnostics for the Zika virus predominantly look...Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components." Cell (2016). PubMed PMID: 27160350...