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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,041 results for: ins

Showing: 641 - 660 of 1041 results

  1. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...system less bulky and easier to use with other strains of E. coli, Schmidl et al. condensed the system...ensures that they can be used with many types of proteins, including Cas9 and dCas9-transcription factors... optimal. These tools are compatible with many strains of E. coli, as well as with other plasmid-based...

  2. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...available from your institution, your department, and online. The Howard Hughes Medical Institute (HHMI) offers... to an end and your next professional adventure begins. While many career pathways exist for scientists...

  3. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...succeed in communicating. Even the most rigorous, insightful, and novel scientific research will be wasted...research proposal is the perfect fit to their institutional focus and that you're better equipped than anyone...with ComSciCon, find us on Twitter, Facebook, Instragram, or send an email to [email protected]. Many...

  4. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...at the level of nucleosomes, loops, chromosomal domains, or how stretches of DNA on different chromosomes...for the structure of paraspeckles. Clemson CM, Hutchinson JN, Sara SA, Ensminger AW, Fox AH, Chess A, Lawrence... Eric J. Perkins...

  5. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...interaction between neuroscience and other fields. For instance I met one researcher studying how the microbiome...’ve developed in your current work to make new insights in neuroscience. Even if you're currently a synthetic...select viral vectors in the repository, you can instead have Addgene make the virus for you. Neuroscientists...

  6. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...impractical for small projects. Most researchers instead turn to so-called “mismatch nuclease” assays (e.g...then performed to amplify the target using PCR. Instead of tracking the reaction progress as in real time...your genome edits, this paper provides detailed instructions on how to design such assays, in addition to...

  7. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...Jennifer Doudna’s lab at the Innovative Genomics Institute. What is CasPEDIA? CasPEDIA is an online, searchable...structure junky who just wants to learn about Cas domains. CasPEDIA either directly has this information ... More resources from the Innovative Genomics Institute      CRISPRpedia IGI’s digital resources in...

  8. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ... ABEs are only weakly compatible with many Cas domains beyond SpCas9. In order to expand the gene-editing...The latest ABE from the Liu lab is ABE7.10 which contains the deoxyadenosine deaminase, TadA-7.10 (Gaudelli... of naturally occurring enzymes and functional domains speed up the development towards faster and more...

  9. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...protocols for educational resources, and labs and institutions for depositors (see below). Even if the user...well as related items (for example, a kit that contains a plasmid with the catalog ID). Depositor suggestions...suggestions provide shortcuts to matching PI labs and institutions. There are many more technical details that ...

  10. The time and cost required to make a plasmid

    Type
    Blog Post
    ... to make a plasmid. Doench’s lab in the Broad Institute has created hundreds of thousands of plasmids,...Table 1.   Of course, this is for the simple insertion of a PCR product into a vector. The time it takes...your experiment. Make your work “high-throughput” Instead of making one plasmid at a time, try constructing...

  11. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...participating in science and engineering. “Fix the Institutions” promotes gender equality in careers through...the importance of analyzing sex, the National Institutes of Health (NIH) now requires that sex be included...curricula. Corporations need to integrate these insights into product design. But eyes have been opened—and...

  12. Lab Automation at Addgene

    Type
    Blog Post
    ...go over caps and push them into micronic tubes instead of pushing them in one by one. It seems very simplistic...Addgene would love to move forward and automate. For instance, vial labeling would be much easier with a Tube...which air brushes the information onto the vials instead of requiring us to put a label on each tube by ...

  13. CRISPR in the Clinic

    Type
    Blog Post
    ...disease and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic...allogenic “off-the-shelf” treatment. In short, instead of using the patient’s own cells that have been...tract infections. The Cas3 enzymes target several strains of E. coli and lethally chop their genomes into...

  14. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...replicate and continue the infection cycle once inside the host. While the virus can still enter (and ...into the host’s genome. However, some adenovirus strains are known to cause respiratory diseases in humans...your centrifugation speed, to cover the centrifuge bins. These have to be added and removed in a biosafety...

  15. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...remodeling. Chromatin is a complex of DNA and histone proteins that compacts cellular DNA into chromosomes and.... 3. Kaati, Gunnar, Lars O. Bygren, and Soren Edvinsson. "Cardiovascular and diabetes mortality determined...Experiments Post Read Other Blog Posts about Fluorescent Proteins  ...

  16. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ... 5) Listen to your supervisor’s viewpoint and brainstorm about mutually beneficial solutions Your supervisor...Look at the problem from their viewpoint, and brainstorm about solutions that will meet both of your needs...important issues in person or over the telephone instead of email. As you cannot see a person’s body language...

  17. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...AAV does not integrate into the host genome. AAV instead relies mainly on episomal expression. Episomes ... Even more creative options involve generating strains of mice that stably express Cas effectors in specific...doi.org/10.1038/s41596-022-00700-y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak...

  18. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...precise modifications of DNA (replacements, small insertions, and deletions) without introducing DNA double-strand...screens such as PRIDICT/PRIDICT2.0 provide valuable insights into pegRNA editing efficiencies for specific ...Parts, L. (2023). Prediction of prime editing insertion efficiencies using sequence features and DNA repair...

  19. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...price point, which should be carefully balanced against the limitations of the assay.      Fig....immersion technique, courtesy of CAR15.) Each droplet contains a primer and probe set for both the gene of interest...only ddPCR provides absolute quantitative data, instead of relative quantification.  Even though no one...

  20. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...cochlea in 3D. The basic configuration of the instrument used in this reconstruction lay the foundation...cleared whole-mount specimens, such as whole mouse brains expressing green fluorescent protein (GFP) (6)....solvent cleared organs) method of imaging rodent brains and spinal cords by combining Ultramicroscopy with...
Showing: 641 - 660 of 1041 results