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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 838 results for: ANG

Showing: 641 - 660 of 838 results

  4. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ... marker in the various cell types of the basal ganglia. They work via newly-characterized enhancers that...

  10. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...localized, and how its localization and expression may change across various conditions. However, this system...

  17. 10 Ways to Share Your Science!

    Type
    Blog Post
    ... outreach. Here in the North Carolina Research Triangle area, scientists give talks and science cafés ...

  18. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...sequences from step B.1 into the oligos below. Do not change the ends; these bases are important for cloning...CO 2 for 12-15 hours. Day 3: j. In the morning, change the media to remove the transfection reagent. Replace...Lentiviral particles can efficiently infect a broad range of cell types, including both dividing and non-dividing...to cells. Days 3+: e. Examine cells each day and change to fresh puromycin-containing media every other...Target cells should be approximately 70% confluent. Change to fresh culture media containing 8 μg/mL polybrene...particles per cell. Addgene recommends that you test a range of MOIs to determine the optimal MOI for infection...Incubate cells at 37°C, 5% CO 2 overnight. Day 3: e. Change to fresh media 24 hours after infection. TIP: If...

  19. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...determine when the desired color change has occurred. The color will change from yellow to blue and generally...experimental conditions. When the desired color change has occurred, gently remove the plate seal. Using...generate a standard curve . If you don’t see any color change or dose response, your antibody concentration may...case, use lower antibody concentrations. The ideal range of the standard curve will vary between targets ...your unknown sample’s absorbance falls above the range of the standard curve you will need to either include...
Showing: 641 - 660 of 838 results