We narrowed to 1,071 results for: CAN

...Davidson lab for the last four years. “Researchers can make their own target if it is not already made for...backbone with very similar restriction enzymes. You can essentially cut and paste quite quickly. Researchers...included some of their own reference images that can be found on the corresponding plasmid pages (e.g....Maserati. If we know how all the parts go together, we can fix it. Right now, we have no idea." Now, it will...story about his life’s work and the stage IV lung cancer that has now prompted him to deposit the tools ...

...are not aware of what Addgene can do for them and how the repository can help them share their materials...makes her the perfect Addgenie and she can’t wait to see how she can help the scientists in Europe with their...engagement. Information about contacting Addgene can be found on our Contact page. You’ve called. You’...Molecular Biology. She has worked in a range of labs - cancer research, microbiology, virology, and genetics ...

...variants of unknown significance” (sometimes called “VUS”) function is unknown. How can we make a decision...morphological profiling can be used as generalizable strategies to map the function of rare cancer variants and ...functional genomics a reality for future cancer patients. You can follow him on Twitter @boehmjesse.   Additional...Accelerator Plasmid Collection, that we hope researchers can use to characterize these variants in a high-throughput... proven to improve future clinical decisions, we can work together to realize the potential of precision...Collection) or “pan-cancer” analyses across many cancer types (Pan Cancer Mutant Collection). In addition...Director of the Cancer Program at the Broad Institute of Harvard and MIT. The notion of cancer precision medicine...

...Tips on drafting a stellar resume and cover letter can be found on the Addgene blog.   3. Personal statement...in doubt, consult the web to find the tools that can help you craft a solid personal statement.  4. Letters...a network of friends, and future colleagues that can help pull one another up while you are climbing the...outcome for the both of you. Next year, your friend can circle back and lend you a hand to get you into that...communities to educate parents about resources that can help their children thrive academically. Rose is ...doors to a web of opportunities that the classroom cannot offer. Furthermore, companies across most industries...Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard. Roo...

...transmission in brain slices from mouse, and showed that it can alter motor responses in C. elegans. Find the split...through a host organism, the expression of some genes can change depending on the location. Current methods...given cell type is already known, these reporters can help define the different cells present in a mixed...developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes in any immune lineage without affecting...the anticipated effect of the targeted gene. CHIME can also be used for high throughput screens using pooled...

...lines. Instead, researchers can generate and maintain transgenic lines that can be used for other experiments...specific protein of interest. This fusion protein can then be co-expressed in essentially any transgenic...designed and optimized with zebrafish in mind, it can easily be applied to other systems.    Find BLITZ...limitations of number of flourescent proteins that can be inserted into a cell without disrupting its functions...

...experiment then you will need to make sure that you can easily distinguish each different type of antibody...antibody and therefore each target protein. This can be accomplished by using a panel of primary antibodies...the assay is a unique isotype or species, then you can choose different fluorophore-conjugated secondary...have a number of options. If this is the case, you can narrow them down in a variety of ways. First, review...and experimental considerations - no other factors can make up for an antibody that doesn't work for your...hundreds of options. With limited time and budget you cannot test them all, so how do you know which ones to...

...enzymes implies that even a minute quantity can significantly reduce the operational time for a given process...and it’ll undoubtedly be quite a while before we can firmly gain a handle on understanding and appreciating...perspective, the enzymes obtained from psychrophiles can be used for a diverse array of processes. The food... benefits for any food processing industry as it can potentially help minimize the incidental losses like...suboptimal conditions. This means recombinant proteins can aggregate or precipitate out as insoluble granules...

... the hosts, Josh and Dan, discuss how scientists can start using Twitter and provide real-life examples...for more tips on using Twitter as a scientist. You can also find Dr. Page and Hello PhD on Twitter: @ThePurplePage...not capture your interest during that week.” You can also find Dr. Knoepfler on Twitter: @pknoepfle 3....do list. This video shows quick ways social media can fit into a researcher’s work day. It’s also important...these links, I’ve: 1) convinced you social media can positively impact your career, 2) given you tips ...Social Media for Science Communication” at the American Society for Cell Biology (ASCB) 2018 Conference...

...our chromosomes is very much 3-dimensional, as you can see by this post’s chromatin illustrations from Leah...phenomenon, there’s a reasonable good chance that Addgene can help. Though it would be difficult to consolidate... plasmids containing the indicated gene, and you can subscribe to these pages to learn when Addgene receives...have many different variant names, not all of which can be listed here. If you don’t see the gene you’re ...of plasmids from the indicated publications. You can request all of these plasmids directly from Addgene...potentially detract from the process being depicted, we can’t forget that those other components are still there...

...itself is ~100 bases) limit the number of gRNAs that can be packaged together in a single vector. In addition...protoplasts (15% and 9% of target sites.) How many loci can Cas9 modify? To test the application of this system...frequency at the four loci, indicating that Cas9 can be guided to at least 8 distinct loci. They also ...The use of multiple gRNAs targeting a single gene can increase the likelihood of gene knockout, or multiple... genes with overlapping activity. Multiple gRNAs can also facilitate deletions of other regions, such ...

...read particular studies or experiments. Later, you can easily convert the citation style to whatever the... relevant researchers in the field. Finally, you can sound credible and cool when you casually mention...factorials. In environmental science and ecology, this can be represented by the tip of the iceberg. For writers... like me, you might be surprised at how much you can get done between office hours and experiments. A ...don’t forget to include citations so that people can go back and read the original reference for the data.... Submit your article! Overall, writing a review can be overwhelming and challenging. My best advice is...development, physiology and malignancy." Genes & cancer 6.5-6 (2015): 184. PubMed PMID: 26124919. PubMed...

...three stop codons, meaning that the same amino acid can be encoded by more than one codon. Although the genetic...organism's codon bias. The idea is that scientists can make mutations throughout a gene of interest based...certain systems, synonymous changes to a gene sequence can cause unexpected detrimental results to the protein...those that exhibit wobble base-pairing (a tRNA that can recognize multiple synonymous codons) may actually...genes contain a region where a synonymous mutation can be harmful (Sauna and Kimchi-Sarfaty, 2011).  Overall...CDS-located miRNA target sites suggests that they can effectively inhibit translation. Genome Research ...expression limiting regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten...

...both dCas9 and the gRNA. This expression can vary significantly depending on transgene design and delivery...proteins might not localize to the right places, which can provide misleading information on where exactly a...signal or including fusions with Histone 2A or 2B can result in bright targeted expression while minimizing... found that too high expression of these markers can lead to non-specific labeling. They generated plasmids...be exported to the periplasm of bacteria where it can be secreted into the supernatant. Four plasmids containing...-one dCas9-KRAB and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for...benefits of nanobodies over conventional antibodies can’t be overlooked. Nanobodies are smaller in size, more...

...Such a behavior can be monitored in an open field test (Alpar et al., 2018). Locomotion can also be used ...an animal running to escape a threat. Locomotion can thus be used as a readout for associated emotional...advantage of these characteristic traits, rodents can be used to study anxiety levels in general, or anxiety...maze that measures risk aversion/anxiety. The mouse can move freely on a 4-arm cross and choose to stay on...recombinase-expressing ready-made AAV vectors which can be used in numerous floxed animal species for behavioral...

... another virus. With the pseudovirus, scientists can study the mechanisms of infection and how to interfere...yields and stability. This stabilized spike protein can be used for the development of vaccines and diagnostics...those encoding different spike protein variants that can be incorporated into pseudoviruses. The lab used ...neutralizing the different SARS-CoV-2 spike variants. You can learn more about their research in this article. ...transcription (RT-LAMP) and a colorimetric readout that can be completed in just 45 minutes. The team deposited...MMLV reverse transcriptase and Taq polymerase that can be used in a one-step RT-qPCR reaction to detect ...

... only the highly restricted number of people who can access the ELN have the ability to attempt to reproduce...kind of informal conversations about research that can take place in Slack normally never become part of...into ELNs would also be useful, so that the data can be viewed – and conceivably additional work relating...relating to reproducing the original research can be documented -- in the original context in which it was...analyzed. While the many different ELNs out there can help with searchability, record-keeping, and data...through interacting with colleagues in Edinburgh. You can follow Rory on Twitter @rory_macneil.     Additional...with a particular experiment or project thus significantly enhances the ability of others to subsequently...

...managed! The good news is that management skills can be taught, but it will take time so be patient and... learn what motivates them (keep in mind that it can be very different from what motivates you!), know...important is to be able to actively listen - this can be hard - but it is the best way to avoid mistakes...The silence and maybe even the slight awkwardness can spur people to speak up. Once aware of the problem...want to know. Remember that one on one discussion can be confidential. Finally, provide timely feedback...will be grateful for the feedback, but at least you can start making changes for the best. Positive feedback...

... lab has worked extensively with enChIP systems. Can you describe this technology and its advantages? ...enChIP). By using enChIP, specific genomic regions can be easily isolated for identification of associated...retroviral enChIP system using CRISPR technology. Can you describe this technology and some of its potential...involved in these professional activities because I can contribute by, for example, helping to improve the...and Japan (University of Tokyo, Osaka University). Can you speak about your different experiences in research... in Japan, which is very disappointing. I hope I can help change the situation in the future. Addgene:...

...internal region of the mRNA. In the figure above, you can see that the IRES element acts as another ribosome... L L K L A G D V E S N P G P * (GSG) residues can be added to the 5' end of the peptide to improve ... the co-expression of multiple unique genes, you can start with a plasmid that has multiple cloning sites...PCR-cloned between your genes of interest and you can then insert the whole multicistronic cassette into... 101 eBook And now for some exciting news... You can now download Addgene's new eBook! Our goal was to...additional resources into one downloadable PDF that you can save on your desktop. The Plasmids 101 eBook is designed...