...academic vs. industry postdoc  Time: Academic postdocs can be long—5 years or more, especially in the biological...AstraZeneca, this is three years. This time limit can feel like a lot of pressure. However, it means that...has ties to industry, the drug discovery process can be a bit of a mystery to graduate students in academia...immersed in that environment sooner rather than later can be helpful. Publishing: As a postdoc at AstraZeneca...AstraZeneca, you are expected to publish, but this can vary depending on which company you choose. If publishing... In this scenario, finding someone with whom you can have an in-depth discussion about your project might...have less time to explore scientifically since you can’t afford to spend too much time working on something...

...and the mice. While not an exhaustive list, these can include what’s being injected, the type of experiment... and restraint method. Many different substances can be injected, including cells, drugs, DNA, viral vectors...to these as “tail vein injections.” However, they can also be given retro-orbitally, behind the eye. IV...technique, as it requires intubation of the mice, which can be tricky given their small size. This technique ...nose, they also pass through the upper airways and can target the airways before the lungs. This is most...tears or punctures to the throat. The feeding tube can be flexible or metal, depending on researcher preferences...

...Using their PITCh plasmids, GFP knock-in cell lines can be created in about a month and a half, without the...In contrast, the short homology required by MMEJ can easily be added via PCR amplification. Given the ... is a robust method for genomic insertion. PITCh can also be adapted for whole-plasmid integration if ...expressing GFP from a given promoter, and the technique can be adapted to other transgenes. It’s important to... of MMEJ represents another strategy researchers can exploit for CRISPR gene editing. In organisms where...interesting to see what new editing possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (...

...functional composition. In other words, these parts can be used over and over again in combination with novel...wants to develop a language for programming life. We can work backwards and say for this to be successful ...still missing? Endy: That's a great question. You can see that we have the beginning of control around ...mostly to the imagination. The question is: what can you use biology to make? If you think about the BIOFAB...someone to forward engineer a system of 10 genes. You can now forward engineer a 10-gene system to do something...to its users and to the design community: If you can begin to reliably put together 10-gene systems, what...

...to order plasmids she can use as positive controls in PCR-based cervical cancer screens. This third grant...these screens can then undergo more routine screening allowing for early cervical cancer detection, treatment...that Gladys will be using plasmids in a way that can potentially have a direct impact on human health .... Cervical cancer screening in Ghana Gladys is currently working to enhance cervical cancer screening ...wrote in her application, “Cervical Cancer is the leading cause of cancer related morbidity and death among...developing countries” and “Almost all cervical cancers are caused by persistent infection of HR HPV types...To start her work, Gladys will improve cervical cancer screening for a limited group of Ghanaian women...

... is our own immune system, which can recognize antigens on cancerous cells and eliminate them from the...Tackling Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used...suppressors and upregulation of proto-oncogenes ­­­can directly inhibit the ability of a cell to undergo...demonstrated that a number of novel small molecules can bind directly, albeit weakly, to K-Ras and inhibit...list of all available Addgene plasmids he or she can use to study it (as seen in the screenshot below)...Additional Resources National Cancer Institute: Cancer Statistics Page National Cancer Institute: Ras Pathway...tens of millions of new cancer cases arise and millions of people die from cancer-related complications ...

...Argonaute. They identified NgAgo and showed that it can cleave plasmid and genomic DNA in a variety of mammalian...DNA guides may be more user-friendly, since they can be ordered as oligonucleotides and should be stable...transcribed, and changes in gRNA secondary structure can affect editing efficiency. Gao et al. tested if NgAgo...key advantage over Cas9, for which gRNA sequence can greatly affect editing efficiency. Smaller size ... so NgAgo remains faithful to its guide DNA and cannot switch guides. While this fidelity would prevent...

...Running a Western blot can be a bit daunting for first-timers. That's why we gave our written protocol...share tips for this tricky application.    You can check out all of our protocols on our Protocol page...

...increased choice and flexibility; while ensuring we can maintain high standards for both our depositors and...banner on their material page. Interested scientists can expand the banner to select their serotype of choice...’d like, and submit the request. Request details can be accessed within your Addgene account. Preps will...Request prep 6–9 weeks after placing your order.  Can I order Packaged on Request preps through a distributor...packaged DNA and serotype. Please note that Addgene cannot perform functional or in vivo testing. We recommend...

...many years of study to come. Some Freshers Fairs can include fairground rides and other fun activities...between undergraduates and PhD students so that they can help support each other and provide advice. The society... develop a network of fellow scientists that you can grow with, learn from, and mentor while pursuing ...provide strong foundations upon which young scientists can build powerful networks and we encourage them them...

...et al. demonstrate that this new vector, pSiM24, can also be used with biolistic methods, making it useful...controlled by a weaker constitutive Nos promoter, and it can be used to select both transformed Agrobacterium ... are suitable for many applications, the plasmid can also be customized due to its modular structure. ...Given its versatility and customizability, pSiM24 can be used not only for plant engineering and biotechnology...

...Davidson lab for the last four years. “Researchers can make their own target if it is not already made for...backbone with very similar restriction enzymes. You can essentially cut and paste quite quickly. Researchers...included some of their own reference images that can be found on the corresponding plasmid pages (e.g....Maserati. If we know how all the parts go together, we can fix it. Right now, we have no idea." Now, it will...story about his life’s work and the stage IV lung cancer that has now prompted him to deposit the tools ...

...are not aware of what Addgene can do for them and how the repository can help them share their materials...makes her the perfect Addgenie and she can’t wait to see how she can help the scientists in Europe with their...engagement. Information about contacting Addgene can be found on our Contact page. You’ve called. You’...Molecular Biology. She has worked in a range of labs - cancer research, microbiology, virology, and genetics ...

...variants of unknown significance” (sometimes called “VUS”) function is unknown. How can we make a decision...morphological profiling can be used as generalizable strategies to map the function of rare cancer variants and ...functional genomics a reality for future cancer patients. You can follow him on Twitter @boehmjesse.   Additional...Accelerator Plasmid Collection, that we hope researchers can use to characterize these variants in a high-throughput... proven to improve future clinical decisions, we can work together to realize the potential of precision...Collection) or “pan-cancer” analyses across many cancer types (Pan Cancer Mutant Collection). In addition...Director of the Cancer Program at the Broad Institute of Harvard and MIT. The notion of cancer precision medicine...

...our chromosomes is very much 3-dimensional, as you can see by this post’s chromatin illustrations from Leah...phenomenon, there’s a reasonable good chance that Addgene can help. Though it would be difficult to consolidate... plasmids containing the indicated gene, and you can subscribe to these pages to learn when Addgene receives...have many different variant names, not all of which can be listed here. If you don’t see the gene you’re ...of plasmids from the indicated publications. You can request all of these plasmids directly from Addgene...potentially detract from the process being depicted, we can’t forget that those other components are still there...

...transmission in brain slices from mouse, and showed that it can alter motor responses in C. elegans. Find the split...through a host organism, the expression of some genes can change depending on the location. Current methods...given cell type is already known, these reporters can help define the different cells present in a mixed...developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes in any immune lineage without affecting...the anticipated effect of the targeted gene. CHIME can also be used for high throughput screens using pooled...

... the hosts, Josh and Dan, discuss how scientists can start using Twitter and provide real-life examples...for more tips on using Twitter as a scientist. You can also find Dr. Page and Hello PhD on Twitter: @ThePurplePage...not capture your interest during that week.” You can also find Dr. Knoepfler on Twitter: @pknoepfle 3....do list. This video shows quick ways social media can fit into a researcher’s work day. It’s also important...these links, I’ve: 1) convinced you social media can positively impact your career, 2) given you tips ...Social Media for Science Communication” at the American Society for Cell Biology (ASCB) 2018 Conference...

...enzymes implies that even a minute quantity can significantly reduce the operational time for a given process...and it’ll undoubtedly be quite a while before we can firmly gain a handle on understanding and appreciating...perspective, the enzymes obtained from psychrophiles can be used for a diverse array of processes. The food... benefits for any food processing industry as it can potentially help minimize the incidental losses like...suboptimal conditions. This means recombinant proteins can aggregate or precipitate out as insoluble granules...

...Tips on drafting a stellar resume and cover letter can be found on the Addgene blog.   3. Personal statement...in doubt, consult the web to find the tools that can help you craft a solid personal statement.  4. Letters...a network of friends, and future colleagues that can help pull one another up while you are climbing the...outcome for the both of you. Next year, your friend can circle back and lend you a hand to get you into that...communities to educate parents about resources that can help their children thrive academically. Rose is ...doors to a web of opportunities that the classroom cannot offer. Furthermore, companies across most industries...Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard. Roo...

...lines. Instead, researchers can generate and maintain transgenic lines that can be used for other experiments...specific protein of interest. This fusion protein can then be co-expressed in essentially any transgenic...designed and optimized with zebrafish in mind, it can easily be applied to other systems.    Find BLITZ...limitations of number of flourescent proteins that can be inserted into a cell without disrupting its functions...