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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 644 results for: CAR

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Showing: 641 - 644 of 644 results

  1. Molecular Cloning Techniques

    Type
    Guide
    ...Restriction enzyme cloning also leaves behind a short scar in the DNA sequence and can be time-consuming compared...entire cloning step (digestion and ligation) can be carried out in one reaction with a single type IIS restriction... the final product, with no undesired sequence ("scar") retained. A disadvantage of type IIS assembly ...

  2. Guide to Using Pooled Libraries

    Type
    Guide
    ... easy identification of the shRNA a given cell carries. Addgene currently does not distribute any shRNA...results – for a given plasmid, think of each cell carrying it as being a biological replicate for that plasmid...

  3. Modular Cloning Guide

    Type
    Guide
    ...Cloning System and Toolkit Yeast Expression Elena Caro Bernat Plasmids for generating transcriptional units...Kit Bacterial Expression, CRISPR Veronica Godoy-Carter Plasmids for protein expression and CRISPRi experiments...

  4. Plan Your Experiment

    Type
    Guide
    ... producing stable cell lines, since the genetic cargo (in this case, CRISPR components) integrates into...
Showing: 641 - 644 of 644 results