We narrowed to 644 results for: CAR

1. Molecular Cloning Techniques

   Type

   Guide

   ...Restriction enzyme cloning also leaves behind a short scar in the DNA sequence and can be time-consuming compared...entire cloning step (digestion and ligation) can be carried out in one reaction with a single type IIS restriction... the final product, with no undesired sequence ("scar") retained. A disadvantage of type IIS assembly ...

2. Modular Cloning Guide

   Type

   Guide

   ...Cloning System and Toolkit Yeast Expression Elena Caro Bernat Plasmids for generating transcriptional units...Kit Bacterial Expression, CRISPR Veronica Godoy-Carter Plasmids for protein expression and CRISPRi experiments...

3. Plan Your Experiment

   Type

   Guide

   ...save your lab valuable time and resources when carrying out CRISPR experiments and can be used as positive... producing stable cell lines, since the genetic cargo (in this case, CRISPR components) integrates into...

4. Guide to Using Pooled Libraries

   Type

   Guide

   ...results - for a given plasmid, think of each cell carrying it as being a biological replicate for that plasmid...