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We narrowed to 957 results for: Cre

Showing: 641 - 660 of 957 results

  1. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...made use of mazF as a counterselectable marker to create unmarked genetic modifications in the yeast Pichia...counter selection in one plasmid, they were able to create an efficient cloning tool. The plasmid pKSCTMF ...Morin and James Kaper used the hok/sok system to create strains that can stably express antigens in sufficient...

  2. How to Write a Scientific Review Article

    Type
    Blog Post
    ...researchers in the field. Finally, you can sound credible and cool when you casually mention “Haery et al... al., showed that MYC expression was increased…” when discussing the review topic with your peers. Start...will also paraphrase what I have heard many other creative people say about writing: you don’t know what ...

  3. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...prognoses. Survival rates for mesothelioma are creeping upwards, and while most people still die within...world. New mesothelioma cases could be greatly decreased through a ban on asbestos. Nonprofits like the...Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana Additional Resources on Addgene.org ...

  4. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...glutathione reductase to faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...

  5. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...you need to speak to start creating slides or thinking about content. Create a Google.slides or Powerpoint...capture interesting key results, striking images, creative ideas, and engaging stories as you encounter or...

  6. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...percentage of dystrophin-expressing muscle cells increases over time, and all three groups confirm dystrophin...muscle stem cells are modified by this approach, increasing the possibility that edits will persist long-...off-target risk must be evaluated separately, increasing the time to clinical approval. Even with the ...

  8. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...fluorescent dyes, and fluorescent ligands. Figure created with biorender.com. Fluorescence refers to...fluorescent protein tags, such as higher specificity, increased versatility in probe attachment, and compatibility...exciting the fluorophore at peak wavelength can also decrease the rate of photobleaching. Interaction with receptors...

  9. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...of antibody validation, and it is recommended to screen multiple candidate antibodies when possible. If...interest. By correlating your data sets, you can increase confidence that the labeling observed represents...experiment. We find it useful to start with a screening procedure in easily genetically modified cell ...

  12. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...various CRISPR applications (such as visualize, screen, activate, and repress) on Thursday. We’d also ...from nickases to base editing to pooled library screening. It also has information for planning your CRISPR...

  13. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ...platform changes, such as limiting access and increased priority given to paid content regardless of user...preserve the knowledge, conversations, and community created and shared on our feed, we will not delete @addgene...

  14. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ...celebrations! We're kicking it off by sharing all the incredible guest posts and stories featured in our blog ...Plasmid Request Data Author: Joachim Goedhart  Created in collaboration with Addgene writers High Complexity...

  15. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    ...and Instagram and we’re excited to share these creative entries as the winners of the 2019 contest. ...material tree We often get submissions of decorations created from lab materials. This year, one of the winners...

  16. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ... guide that curates this information. And so I created the Codon Guide to Synthetic Biology in the hopes...open-access resources whenever possible.  Image credit: Niko McCarty; adapted from Bruce Wetzel and Harry...

  18. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies for use in mice and human...from 2022! Wow! Our depositors do some really incredible science - I hope you've enjoyed learning about...

  19. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...Microbiology Collection are sorted by organism (see screenshot above). Don’t see your favorite organism listed...Addgene has so many of these resources that we created filters just for them on our search page. Use the...
Showing: 641 - 660 of 957 results