We narrowed to 967 results for: Cre

...each project. This creates accountability on the part of each developer and creates trust amongst the ... Addgene grows and evolves, we will be here to increase all of our teams’ efficiency and productivity....

...and multiple gRNAs into separate AAV vectors, increasing overall packaging capacity but necessitating ...efficiency of AAV-SaCas9 in vivo, the Zhang lab created an all-in-one SaCas9 and sgRNA construct using ...histologically normal and liver injury markers were not increased compared to a control AAV-GFP. Not only did the...

...1998). As the copy number of the plasmid increases, increased amounts of these antisense RNAs inhibit ...inhibit its own transcription. As copy number increases, RepA transcription is repressed and replication...

...expression transposases leading to increased integration frequency. (C) Increasing rounds of transfer on transposition-inducing...chromosomal integration of cargo without using screening markers. Capability to construct a strain library...

...proteins cooperatively can separate the DNA strands. Created with BioRender.com. Once DnaA breaks the local...helicases to continue unwinding the strands. This creates two replication forks that move in opposite directions...pseudoknot from folding, blocking replication. Created with BioRender.com. Plasmids under stringent...

...researchers in the field. Finally, you can sound credible and cool when you casually mention “Haery et al... al., showed that MYC expression was increased…” when discussing the review topic with your peers. Start...will also paraphrase what I have heard many other creative people say about writing: you don’t know what ...

...glutathione reductase to faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...

...you need to speak to start creating slides or thinking about content. Create a Google.slides or Powerpoint...capture interesting key results, striking images, creative ideas, and engaging stories as you encounter or...

...percentage of dystrophin-expressing muscle cells increases over time, and all three groups confirm dystrophin...muscle stem cells are modified by this approach, increasing the possibility that edits will persist long-...off-target risk must be evaluated separately, increasing the time to clinical approval. Even with the ...

...anything downstream to detect. We ended up having to increase our aptamer concentrations significantly before...a team after everything we put into it was an incredible feeling. We also placed in the Top 10 high school...reason Lambert iGEM has been able to take on increasingly ambitious projects. Each team builds on what...

...should be able to apply someone else's new and incredibly useful technique simply by following the abbreviated...49:45 - 1:18:45), there are also initiatives to create more reliable reagents for future research. I’ve...IPI is working to address this issue directly by creating well characterised antibodies against all human...

...fluorescent dyes, and fluorescent ligands. Figure created with BioRender.com. Fluorescence refers to...fluorescent protein tags, such as high specificity, increased versatility in probe attachment, and compatibility...exciting the fluorophore at peak wavelength can also decrease the rate of photobleaching. Interaction with receptors...

...of antibody validation, and it is recommended to screen multiple candidate antibodies when possible. If...interest. By correlating your data sets, you can increase confidence that the labeling observed represents...experiment. We find it useful to start with a screening procedure in easily genetically modified cell ...

...is a significant hurdle made worse by an ever increasing marketplace. Since 2013 about 11 tools have been...the user is expected to interact with the tool (a screen shot and overview of the table can be found above...re engaged in heavy CRISPR design (I’m talking screens and/or very large genomes), then it is worthwhile...

...for controlling protein activity, methods for increasing CRISPR editing efficiency, new CRISPR base editors...Segment  Hot Plasmid 2: Ouabain selection for increased CRISPR editing efficiency. Listen to the Segement...

...platform changes, such as limiting access and increased priority given to paid content regardless of user...preserve the knowledge, conversations, and community created and shared on our feed, we will not delete @addgene...

...various CRISPR applications (such as visualize, screen, activate, and repress) on Thursday. We’d also ...from nickases to base editing to pooled library screening. It also has information for planning your CRISPR...

...celebrations! We're kicking it off by sharing all the incredible guest posts and stories featured in our blog ...Plasmid Request Data Author: Joachim Goedhart  Created in collaboration with Addgene writers High Complexity...

...and Instagram and we’re excited to share these creative entries as the winners of the 2019 contest. ...material tree We often get submissions of decorations created from lab materials. This year, one of the winners...

... guide that curates this information. And so I created the Codon Guide to Synthetic Biology in the hopes...open-access resources whenever possible.  Image credit: Niko McCarty; adapted from Bruce Wetzel and Harry...