...microbes to visualize host/pathogen interactions.   Do you have a favorite way to use GFP?  Comment and ...

...replication-competent lentivirus (RCL) in your target cells.  Q4: How do the packaging systems differ between 2nd- and 3rd-generation...

...transformation . Tips and FAQ Do controls When doing ligations you should ALWAYS do a vector alone + ligase ...you know has only been thawed once before. Always do controls. See Tips and FAQ below for details. Try...situations where the 3:1 ratio is not working or when doing more complicated cloning. While 3:1 will get you...

...total plasmid DNA. Tips and Troubleshooting What do I do if my transformation efficiency is not high enough... Pooled Libraries Molecular Biology Reference How do I process my Addgene pooled library? Introduction...Endura from two separate transformations). Pro-Tip Do not pipette repeatedly or mix when removing SOC containing...Critical Be careful not to rip or shred the agar. Do so by gentle spreading. Some spreaders have a sharp...Maxi Kit (one conical is its own Maxiprep). Critical Do not freeze pellets for later purification. Immediately...colder reagents (not including the recovery media!). Do not proceed with Maxipreps or NGS until adequate ...representation is maintained. Maxipreps - Less is more: Do not overload the Maxipreps as yield can dramatically...

...single molecules - something researchers often want to do at neuronal synapses for instance. Also these tags...

...systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host...

...your sample names and guide sequences, and ICE will do the rest. There are no parameters that need optimizing...

... spin columns you need to complete your research. Do you know of other nucleic acid purification methods...

...high-quality data for our DNA-based reagents. Not only do we extract as much information as possible from depositing...

...in the biosynthetic pathway. Why plant scientists do transient leaf transformations The development of...

...Hoshino and Fujii, 2009). Other forms of enChIP, that do not use dCas9, require Zinc-finger or TAL proteins...

...your research, be sure to consider how its features do and don’t support reproducibility as not all ELNs...

...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant...

... GetSet Need a set of overhangs, stat? GetSet can do that for you, complete with predicted fidelity. You...

... pooled libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled...

... become valuable tools for your research! If they do (or if they don't!), please consider sharing your...

..., Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes cavitation....27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to measure and...

...ddPCR for AAV quantitation blog post.  We generally do not recommend using ELISA-based titering assays, ...determination of the median tissue culture infective dose (TCID50) (Zen et al., 2004).  To choose the optimal...

...evaluate tens of thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting...

...explore. I hope that this brief guide can help you do just that. Good luck, and always keep going. Appendix...