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  1. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...pSiM24, can also be used with biolistic methods, making it useful for engineering plants that are not susceptible...available pCAMBIA and pKYLX vectors, respectively, making it ideal for large gene transfer. The M24 promoter...

  4. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...collaboration. This is why she is excited to be working at Addgene, because it brings scientists together...others. This love of interacting with people and talking about science makes her the perfect Addgenie and...

  7. An Addgene Summer Internship

    Type
    Blog Post
    ...her talk about Addgene’s internship opportunity taking place next summer.  I applied to Addgene and began...week at Addgene. This report shared my work by breaking down the process of each day, my background, observations...

  8. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...not be expressed in the tissue/cell line you are working with. Development-specific genes – if a gene...depends on your experiment and the organism you are working in. The spacing of genes and whether or not it’...

  9. Hot Plasmids - October 2022

    Type
    Blog Post
    ...selectivity for K+ over Na+ and rapid photocurrent kinetics. They demonstrate the use of HcKCR1 as an optogenetic...photocurrents, and is more selective for K+ than HcKCR2, making it more suitable for in vivo optogenetic use.   ...

  12. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ... to improve our resources for this community. Networking with microbiologists Over the course of the conference... technology. Addgene got several shout-outs for making popular screening libraries like Brie and Brunello...

  13. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ... be. Managers must ensure that their teams are working well together and collaborating effectively with...ability to assess and initiate things independently. Taking initiative doesn’t mean going rogue, pointing out...

  14. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...some people find this hard so get used to it by making it a concrete goal to say “thank you, job well ... they are at risk of being fired and should be looking for a new position. One of the most common mistakes...

  15. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...application. Depending on the tag, directly imaging or tracking your protein may also be possible, without the...also be removed by treating the protein with enterokinase, which recognizes the 5 amino acids on the C...

  16. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...You are a scientist looking to determine how Protein A and Protein B interact. You read extensive research...Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., & Scher, D. S. (1994). Isotype...

  17. What the HEK?

    Type
    Blog Post
    ... four. No Y chromosome is present in this line, making it genetically female. HEKs are generally cultured... line for you. Are HEKs for you?   If you are looking for a human cell line model, HEKs might be a great...

  18. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...by outright stating the big-picture problem, by asking your audience a simple question, like “Have you...ending of the story.  At this point you may be thinking  “I don’t want to bury my results all the way ...
Showing: 641 - 660 of 926 results