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We narrowed to 1,027 results for: LEA

Showing: 641 - 660 of 1027 results

  1. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...search tool updates. Now, let’s take a look at the least visible part behind these changes: the backend technology...engine! Additional resources from the Addgene blog Learn about other features on Addgene's website Find a...step-by-step guide to using Addgene's new search Read to learn more about why we redesigned our homepage and mascot...

  2. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ... Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder, Melina Fan, we created...Production webpage). While the new team took the lead on operationalizing viral production and distribution...Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral Service in Your Research...

  3. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...This is just a primer so that you are aware of at least some of the possibilities. Basically, if you can...between pathogens and their hosts. It’s not always clear which side he roots for. You can try to convince...PMC3172133. Additional Resources on the Addgene Blog Learn about Using Cre in Drosophila Read Other Genome ...

  4. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...receptor-transcription factor fusion. TEV protease cleave the transcription factor from the receptor, which... in chemogenetics  While there’s still a lot to learn about GPCR signaling, researchers in the meantime...02775-8 Additional resources on the Addgene blog Learn more about TRUPATH Read our quick guide to chemogenetics...

  5. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...forward-looking. Embrace it, and let your personality leap out of the page. Finally, as previously mentioned...the recommender knows you the better, and their leadership status in their current field of expertise augments...But you create a network of friends, and future colleagues that can help pull one another up while you are...

  7. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...’s wasting everybody’s time if you don’t. Add a clear cover letter to your submission, briefly summarizing...house style and prerequisites of the journal. Ask colleagues (preferably native English speakers) to proofread...that all required information is provided. The clearer you write your paper and the more information you...

  8. Antibodies 101: Conjugation

    Type
    Blog Post
    ...targets. Check out our post on Biotinylation to learn more about biotin and its use in molecular biology...other classes that we would be remiss not to at least mention. Oligonucleotides allow for sensitive detection... with BioRender.com.     Many biologists can lead perfectly blissful, productive lives without thinking...

  9. The time and cost required to make a plasmid

    Type
    Blog Post
    ...people said it took them more than 22 hours, so at least the better part of four days. How much does it cost... a number of factors, but we can assume it’s at least equal to the $155 spent on reagents. Five ways to...tell. But even from these rough estimates, it’s clear that community engagement with and use of repositories...

  10. What's New in CRISPR - November 2021

    Type
    Blog Post
    ... works best for genome editing  with an active nuclease.  Read the paper in Molecular Cell Find the CasMINI...Programmed genome editing by a miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature...protect the RNA from degradation by cellular exonucleases and broadly improve editing efficiency. Read...

  12. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...proteins in situ. I was privileged to be invited to learn from them at the MPI in Frankfurt am Main and perform...concerts and bake. you can contact her at rojas@ti2learning.org. References 1. Dieterich, Daniela C., et al...Fluorescent microscopy techniques - Which is best for me? Learn how small molecule fluorophores can be used for ...

  14. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...within the resistant populations were shared by at least one other replicate; prior to treatment, there are...particularly interested in applying what we have learned about tumor heterogeneity to drug discovery and...PMC4900892. Additional Resources on the Addgene Blog Learn about the Sabatini and Wood Drug Screening Kit Find...

  15. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ... papers is to practice reading them. There’s a learning curve for the jargon and background in your field...the reader to interpret the results to see if it leads you to the same conclusion. Personally, I like papers...interest. Set aside some time each day or week to at least scan the titles of what’s going on in your research...

  16. A Look at Addgene's QC Process

    Type
    Blog Post
    ... high-fidelity polymerases can make mistakes and lead to the accumulation of errors in DNA sequences. ...originally intended. Sharing unverified plasmids can lead to far reaching consequences, from wasting time ...available for distribution. In these cases, the QC team leaves a note on the plasmid page to make requesting scientists...

  17. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...Genes allowing bacteria to survive toxins are the clearest example of a plasmid benefiting its host. For ...engineered entirely new types of plasmids without clear analogues in nature. For example, helper plasmids...plasmid tools available in the Addgene repository. To learn more about some other common plasmid features, such...

  18. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...reprobing, maximize your signal by starting with the least-abundant protein and working toward the most abundant...primary and secondary antibodies from your membrane, clearing the way for the second blot. There are multiple...with the mild one. Reprobe Once your membrane is clean of antibody, you’re ready to repeat your western...

  19. CRISPR in the Clinic

    Type
    Blog Post
    ...cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic potential...of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult...Early-stage trials showed a high success rate in clearing misfolded protein levels as measured in the blood...

  20. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...receptors hijack cell signaling pathways and can lead to such responses as decreased heart rate (Redfern...properties such as endogenous gene expression, which can lead to changes in normal cell physiology and function...posts about neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics guide...
Showing: 641 - 660 of 1027 results