... works best for genome editing  with an active nuclease.  Read the paper in Molecular Cell Find the CasMINI...Programmed genome editing by a miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature...protect the RNA from degradation by cellular exonucleases and broadly improve editing efficiency. Read...

...within the resistant populations were shared by at least one other replicate; prior to treatment, there are...particularly interested in applying what we have learned about tumor heterogeneity to drug discovery and...PMC4900892. Additional Resources on the Addgene Blog Learn about the Sabatini and Wood Drug Screening Kit Find...

... papers is to practice reading them. There’s a learning curve for the jargon and background in your field...the reader to interpret the results to see if it leads you to the same conclusion. Personally, I like papers...interest. Set aside some time each day or week to at least scan the titles of what’s going on in your research...

...Washington DC; convening meetings of experts and leaders from academia, industry, community, education and...the biological sciences, as well as to acquire leadership and communication experience. These included ... science career path Resources on Addgene.org Learn how Addgene engages with the community on our Addgene...

...cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic potential...of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult...Early-stage trials showed a high success rate in clearing misfolded protein levels as measured in the blood...

...receptors hijack cell signaling pathways and can lead to such responses as decreased heart rate (Redfern...properties such as endogenous gene expression, which can lead to changes in normal cell physiology and function...posts about neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics guide...

... high-fidelity polymerases can make mistakes and lead to the accumulation of errors in DNA sequences. ...originally intended. Sharing unverified plasmids can lead to far reaching consequences, from wasting time ...available for distribution. In these cases, the QC team leaves a note on the plasmid page to make requesting scientists...

...Genes allowing bacteria to survive toxins are the clearest example of a plasmid benefiting its host. For ...engineered entirely new types of plasmids without clear analogues in nature. For example, helper plasmids...plasmid tools available in the Addgene repository. To learn more about some other common plasmid features, such...

...reprobing, maximize your signal by starting with the least-abundant protein and working toward the most abundant...primary and secondary antibodies from your membrane, clearing the way for the second blot. There are multiple...with the mild one. Reprobe Once your membrane is clean of antibody, you’re ready to repeat your western...

...Our Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has...Lineage Tracing Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral...

...Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control...Self-complementary AAV mediates gene targeting and enhances endonuclease delivery for double-strand break repair." Gene...

...list. 7) Internships – Learn on the job Nothing prepares you better than learning on the job. Try to get...path you want to follow, check out the distance learning courses at the National Council for the Training...

... “Download Deposit Spreadsheet” (see Figure 1). Please read our “How to Deposit Your Plasmids with Addgene... a collection that is greater than 75 plasmids, please contact us at [email protected]. These deposits...37 °C, or room temperature. Growth Strain Please indicate which growth strain Addgene should propagate...

...final recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide webpage, and references...purified plasmid stock should be analyzed by endonuclease restriction digest or fully sequenced to confirm...a week), and do NOT harvest the cells before at least 10 days of incubation as it will result in very ...

...one is in the making by Dr. Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated...these criteria and deemed positive, it will be released through the website for public reference. The ... sending antibodies, and/or working with Dr. Wu please contact us here! Ava Shipman is a research assistant...

...Kamiyama D. Commun Biol. 2021 Daichi Kamiyama Cerulean Cerulean(1-10) for use with GFP(11) Multiplexed labeling...ECFP C155 of ECFP pairs with N173 derived from Cerulean or Venus Identification of new fluorescent protein...

...the different SARS-CoV-2 spike variants. You can learn more about their research in this article. To see...that are codon optimized. Browse the collection or learn more about it on the blog. COVID-19 research is ....0030 Additional resources on the Addgene blog Learn more about mapping the SARS-CoV-2 - human protein-protein...

...analyzes publications and figures using machine learning to extract important features such as influential...you organize and search for papers later. Pro tip Learn more about authors by exploring author pages (for...literature. Additional resources on the Addgene blog Learn why scientists should be on Twitter Read our Early...

...your questions, data, and conclusions should be clearly identified and interpreted in less than a couple...to read. The key to the success of this style is clear borders around your columns, and good spacing between...used for displaying all of one’s figures, or at least the majority of the visual data. This central box...

...Having never been to China myself, I was pleased to be able to participate when the organizers of the...dozens of different mushroom varieties alone. At least I could tell that they were mushrooms. In some cases...Medicine Initiative and how this initiative will lead to advances in human health. It was inspiring to...