We narrowed to 771 results for: LIS;
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TypeBlog Post...appear to be very effective probes for finely visualising sub-cellular structures or low-abundance proteins...
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Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post... a graphical representation of the results and a list of the analyzed samples (see below). If the sample... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...work — in their system. The data is curated and published with a DOI, allowing others to reference and cite... -
Bioinformatics at Addgene
TypeBlog Post...Creating a bioinformatics workflow in Nextflow Establishing a complete pipeline however, required more than... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...applications of CRISPR. Since this post was originally published, we've also seen the new development of Cas13b... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...have been used successfully in experiments and published in peer reviewed journals. If you need to generate... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...within neuronal networks in living animals. To accomplish such cell specific manipulations in vivo, FLEx... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...it isn’t a good fit for all experiments. And the list of antibiotic options doesn’t end there. Make sure... -
Plasmids 101: Codon usage bias
TypeBlog Post...consider when designing a full gene. A non-exhaustive list includes: Codon abundance relative to cognate tRNA... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post... yourself for potential gRNA sites, or you can utilise several online tools that do it for you. One such... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...your own neuroscience sub-discipline that has published using the technique and ask if they would be willing... -
Synthetic Biology - Overview
TypeCollection...George Church Cynthia Collins James Collins Tom Ellis Drew Endy Tobias Erb Susan Golden John Gray Jeff...Maharbiz Thorsten Mascher Kathleen Matthews Anastasios Melis Aindrila Mukhopadhyay Richard Murray Erin O'Shea..., integration, reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic pathways... Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates , BIOFAB Promoter... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help... -
Microbiology Resources
TypeCollection...for specific genes or applications that are not listed here. Addgene Microbiology Resources Plasmid Kits...sp. Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium sp. Neisseria sp. ...West Nile virus (WNV) Zika Don’t see your field listed? Know of some great plasmid tools for the community...Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help... -
Bacterial Expression Systems
TypeCollection... of Cas9 nuclease gRNA. For a more comprehensive list of our bacterial CRISPR plasmids, see here . pwtCas9...double stranded breaks. For a more comprehensive list of our bacterial CRISPR plasmids, see here . pgRNA-bacteria...disruption or knockdown. For a more comprehensive list of our bacterial CRISPR plasmids, see here . pGRG... and lacO variants that can be expressed in B. subtilis Various combinations of N and C-terminal Strep... express a protein of interest in E. coli , B. subtilis and P. pastoris . All plasmids use the same restriction... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help... -
Arf GTPase Family
TypeCollection...ARF GTPase Plasmids by Gene The following table lists genes in the ARF GTPase family, information on each...available for each gene. Click the gene name for a full list of plasmids available. Please note that some genes...GAP and GEF Plasmids by Gene The following table lists genes in the ARF GAP and GEF family, information... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help... -
Plasmids for Stem Cell Research
TypeCollection...expression of human L-Myc, Lin28, Sox2, Klf4, Oct3/4, Glis1, and EBNA1 from separate plasmids An Efficient Non-viral...from three separate plasmids A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult...expressing human Oct4, Klf4, Sox2, with either c-Myc or Glis1 Efficient generation of human iPSCs by a synthetic...differentiation of iPSCs into cortical and motor neurons. Unpublished. Ward Glial Progenitor Cells GABAergic Interneurons... Research Plasmids This table contains a general list of plasmids that have been used in Stem Cell research... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...system, as is commonly used in western blotting or ELISAs. A popular method for indirect labeling employs... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...principles as other antibody-based assays, like an ELISA or Western Blotting, but it allows you to visualize... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...audience? As Addgene’s Science Video Production Specialist, I’m constantly thinking about ways to improve... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves simultaneously introducing...