We narrowed to 772 results for: LIS;
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TypeBlog Post...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...
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Droplet Digital PCR for AAV Quantitation
TypeBlog Post... of parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...answered the above questions, you can create your short list of options and then pick whichever is most accessible... -
Plasmids 101: Colony PCR
TypeBlog Post...restriction enzyme digest; however colony PCR can accomplish the same thing in less time and for less money... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post... the viral mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as a robust ... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...grant situation. So, young researchers need to publish good publications in a short period of time and... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...it isn’t a good fit for all experiments. And the list of antibiotic options doesn’t end there. Make sure... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...work — in their system. The data is curated and published with a DOI, allowing others to reference and cite... -
Plasmids 101: Codon usage bias
TypeBlog Post...consider when designing a full gene. A non-exhaustive list includes: Codon abundance relative to cognate tRNA... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...safe-targeting guides as a control will result in a smaller list of true positive hits than when using non-targeting... -
Neuronal labeling with Spaghetti Monster
TypeBlog Post...appear to be very effective probes for finely visualising sub-cellular structures or low-abundance proteins... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post... a graphical representation of the results and a list of the analyzed samples (see below). If the sample... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...within neuronal networks in living animals. To accomplish such cell specific manipulations in vivo, FLEx... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...applications of CRISPR. Since this post was originally published, we've also seen the new development of Cas13b... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...Central PMCID: PMC6333924. Howerton, Alexis R., Alison V. Roland, and Tracy L. Bale. "Dorsal raphe neuroinflammation... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...2012) Bacterial plasmid partition machinery: a minimalist approach to survival. Current Opinion in Structural... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...these libraries as ingredients to a recipe. It’s unrealistic to plant, grow, and harvest grains every time... -
Hot Plasmids - August 2020
TypeBlog Post...mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within cells. For example...