We narrowed to 910 results for: Nes

1. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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   Blog Post

   ... power of collaboration, which despite the competitiveness of cutting-edge research, is still one of the...that research is done better with others than by oneself that prompted us to create Crowdfight.   ...

2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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   Blog Post

   ...disrupt target gene sequences (thus inactivating genes), while breaks repaired by homology directed repair... which sequence replacement holds some kind of fitness advantage but error-prone repair of the gene would...

3. Components of CRISPR/Cas9

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   Blog Post

   ...Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...sites with the aid of two RNAs: the crRNA which defines the genomic target for Cas9, and the tracrRNA which...

4. Early Career Researcher Toolbox: Social Media for Scientists

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   Blog Post

   ...interviews on Al Jazeera and NPR, articles in magazines like Fast Company, opportunities to speak at conferences...be wondering how to get started. This blog post outlines 10 steps to take when starting to use Twitter ...

5. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   Blog Post

   ...podcast segment A fundamental approach to studying membranes, organelles, and other subcellular compartments...cause for both the stomach flu and food-borne illnesses, noroviruses can infect millions annually. Because...

6. New and Upcoming Viral Vectors - May 2020

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   Blog Post

   ...inhibition library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening... of the extensive supply of transgenic reporter lines that supply a variety of GFP expression patterns...

7. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   Blog Post

   ... shift requires deliberate preparation and a willingness to embrace new responsibilities. After my second...between technical and managerial roles, and I've witnessed several colleagues do this while changing industries...

8. Flow Cytometry Readouts: Yes, No, and Everything in Between

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   Blog Post

   ...the different populations. MFI is the average brightness from all cells that are positive for the marker...Prathima has a research background in protein mutagenesis and cancer immunotherapies. She is currently ...

9. Addgene’s Viral Service - Why Virus? Why Now?

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   Blog Post

   ...involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave the way for this project...support for export and shipping on dry ice. The Business and Finance Teams continue to facilitate the formation...

10. Sweating the Small Stuff: Details in the Lab

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    Blog Post

    ..., a large set of headphones can act as a Do Not Disturb sign, even if the headphones aren’t actually playing...

11. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post

    ...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...

12. Sharing is Caring: But How to Distribute Open Hardware?

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    Blog Post

    ...microscope has been a powerful tool in almost all disciplines. In biology, innovative new imaging approaches...shows an image of differentiating monocyte with planes and hardware to it. Right panel shows workflow ...

13. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ...Ramsden DA (2014) The fidelity of the ligation step determines how ends are resolved during nonhomologous end...Protocol for Genomic Deletions in Mammalian Cell Lines [Video] Pooled CRISPR Libraries Offer Genome-Wide...

14. RANbodies: Reporter Nanobody Fusions

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    Blog Post

    ...complete. The nanobody based RANbody platform from the Sanes Lab overcomes this limitation and allows for the...its sequence in the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-nanobody ...

15. Transferable Skills Guide: Cross-team Communication

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    Blog Post

    ...words —> decreasing frustration —> increasing the happiness. My unofficial motto in my Product Manager role...effective approaches for clearing up communications lines in your professional life, let me know that, too...

16. Seven Tips for Using LinkedIn as a Scientist

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    Blog Post

    ...can be the best way to get in touch if you have business or follow up. Use the "Contact info" link right...or comment on anything that is not relevant to business or volunteer organizational work. LinkedIn is ...

17. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post

    ... (ChRs) makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing neurons...channelrhodopsins tools have low conductance, but also confines channelrhodopsin activation to a small volume ...

18. Addgene: The First Twenty Years

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    Blog Post

    ...we wanted to walk through some of our favorite milestones and share some thoughts from the community that... From day one, we built systems for now-common business practices, like accepting e-signatures and designing...

19. Human Germline Editing Using CRISPR

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    Blog Post

    ...but this advantage likely won’t transfer to all genes we’d like to target. In addition, as on-target editing...about High Fidelity CRISPR Variants Target Multiple Genes at Once with CRISPR Resources on Addgene.org Browse...

20. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

    ...essential to verify a number of key properties (brightness, photostability, oligomerization) of a number...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...