We narrowed to 903 results for: Nes

...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...

...microscope has been a powerful tool in almost all disciplines. In biology, innovative new imaging approaches...shows an image of differentiating monocyte with planes and hardware to it. Right panel shows workflow ...

...inhibition library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening... of the extensive supply of transgenic reporter lines that supply a variety of GFP expression patterns...

...interviews on Al Jazeera and NPR, articles in magazines like Fast Company, opportunities to speak at conferences...be wondering how to get started. This blog post outlines 10 steps to take when starting to use Twitter ...

...podcast segment A fundamental approach to studying membranes, organelles, and other subcellular compartments...cause for both the stomach flu and food-borne illnesses, noroviruses can infect millions annually. Because...

...involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave the way for this project...support for export and shipping on dry ice. The Business and Finance Teams continue to facilitate the formation...

...Ramsden DA (2014) The fidelity of the ligation step determines how ends are resolved during nonhomologous end...Protocol for Genomic Deletions in Mammalian Cell Lines [Video] Pooled CRISPR Libraries Offer Genome-Wide...

...words —> decreasing frustration —> increasing the happiness. My unofficial motto in my Product Manager role...effective approaches for clearing up communications lines in your professional life, let me know that, too...

...can be the best way to get in touch if you have business or follow up. Use the "Contact info" link right...or comment on anything that is not relevant to business or volunteer organizational work. LinkedIn is ...

... (ChRs) makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing neurons...channelrhodopsins tools have low conductance, but also confines channelrhodopsin activation to a small volume ...

...transparency Another major benefit of preprints is their openness. Preprints can be read freely by anyone and are... work too openly too soon or simply a lack of awareness of the benefits of preprints. Some worry about...

...you cannot test them all, so how do you know which ones to try?  Read on and we will provide you with some...in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody assay...

...methods are often significantly longer than direct ones.  Even though the secondary antibody incubation ...to improve direct methods and avoid the indirect ones. Some Western blots, for example, use a primary ...

...we wanted to walk through some of our favorite milestones and share some thoughts from the community that... From day one, we built systems for now-common business practices, like accepting e-signatures and designing...

...silence–but it was thorough and coherent.  To be honest, if my paper hadn’t been ridiculously more complex... in front of a very confused group of visiting Chinese undergrads. You get nervous and freeze, or forget...

...acid, proteins containing two or more adjacent histidines are present in most production systems and can...can affect protein function. B, The string of histidines in the tag is typically between 6 to 9 residues...

...versatile method to identify the expression of transgenes of interest. Furthermore, fluorophore variants...resulted in abundant expression of hM4Di in plasma membranes in both species (bottom panel in Fig. 1). Our ...

...complete. The nanobody based RANbody platform from the Sanes Lab overcomes this limitation and allows for the...its sequence in the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-nanobody ...

...but this advantage likely won’t transfer to all genes we’d like to target. In addition, as on-target editing...about High Fidelity CRISPR Variants Target Multiple Genes at Once with CRISPR Resources on Addgene.org Browse...

...essential to verify a number of key properties (brightness, photostability, oligomerization) of a number...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...