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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 939 results for: OMP

Showing: 641 - 660 of 939 results

  1. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...decreased p-values of known ricin regulation genes compared to results generated using non-targeting guides...full-length (19-20 bp) or short (17-18 bp) guides. When comparing the toxicity of guides that have 1-bp mismatch...off-target sites, short guides had reduced toxicity compared to full-length guides. Reduced toxicity of short...

  3. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...C) installation by base editors. Pathways that compete with the desired outcome are not pictured. ...Plant base editor platforms and their molecular components. Adenine base editors for plant We developed...editors. Plant CBE and ABE with expanded PAM compatibility All of the base editors mentioned above are...

  4. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...laser cleavage and an RNA aptamer that bound the compound malachite green (MG) to create a non-protein based... 4-hydroxybenzylidene imidazolinone (HBI)-like compound allowing it to fluoresce, the Jaffrey lab asked...production have also made aptamers an important component of high throughput assays with microarrays and...

  5. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...the anticodon,  a three nucleotide sequence complementary to the mRNA sequence. The 3’ end of  the tRNA...resources (Athey et al., 2017) and many DNA synthesis companies, such as IDT, Genewiz and GenScript, offer tools...the other while other codons are under expressed compared to others. Image from Hiss et al., 2017. Most...

  6. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...needs a standard against which the results can be compared; results from an experiment lacking the proper...baseline/standard against which other samples can be compared. The Empty Vector Control (mentioned above) could... where expression of that gene is knocked out completely. These controls may or may not be plasmid-based...

  7. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...promoter present in your plasmid backbone must to be compatible with the type of RNA that needs to be made: if...speaking, promoters in bacteria are less diverse and complex, having fewer parts than those in eukaryotic cells... repression in the presence of glucose or by competitive binding of the anti-inducer fucose Weaker. Commonly...

  8. Pouring LB Agar Plates

    Type
    Protocol
    ...autoclave. Pro-Tip Once your autoclave cycle is complete, we recommend opening the door to the autoclave...

  9. Western Blot

    Type
    Protocol
    ...proteins >150 kDa. Select Start Run. When the run is complete, select Done. Section 5: Blocking Prepare 1X TBST...

  10. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...recombination intermediates can then resolve to complete the DNA repair process Homology directed repair...is elongated and ligated to fill the gap, thus completing SDSA.   Break-induced repair (BIR) pathway BIR... uses reverse transcriptase to generate the complementary ssDNA. Easi-CRISPR was shown to increasing editing...

  11. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...then following transduction, the partial genomes complement each other to restore the full-expression cassette... has equal or higher transgene expression when compared to using a single over-sized vector, but not always... one of the transgene’s endogenous introns or comprised of non-endogenous sequences, such as a 77bp long...

  12. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...get people to remember you). What better way to accomplish all of these things naturally than to give a ...simplicity of their slides and how the slides complement the spoken message. Learn from their level of... topics that are unfamiliar to you because the company you are visiting works on them. Of course, if some...

  13. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...regularly. Dystrophin and genome editing Since complex oligonucleotide treatment comes with many challenges...desirable. Should stem cell editing occur, the CRISPR components would only need to be expressed for a short period... with vastly reduced inflammation and necrosis compared to unedited animals. In grip strength, specific...

  14. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...system, Sanes and Lichtman also developed a complementary Flpbow construct that is functionally similar...technique will provide important insights into the complicated physical organization of the brain and other ...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...

  15. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...stimulation. GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms...applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nature Methods,...

  16. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following a cut... accessibility due to chromatin status). No computational prediction is ever perfect, but this can decrease...to conduct genetic screens, however, a library composed of the latter would likely be more effective, ...

  17. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ... a post-program self-assessment, participants completed follow-up self assessments and program evaluations...knowledge and skills had improved significantly when compared to their pre-program self-assesment. Achievements...other countries. In 2016, we expect the program to comprise over 2000 participants (mentors and mentees). ...

  18. Antibodies 101: Validation

    Type
    Blog Post
    ...validation is so important. Antibodies are a critical component of the researcher toolkit due to their ability...will bind to - particularly when you start using complex samples such as whole cell lysate or tissue sections...BioRender.com.     Orthogonal Strategies This approach compares target expression of antibody-independent approaches...

  19. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...In 1976, the world’s first genetic engineering company, Genentech, was founded.  This commercialization...instead of recognizing nucleotide triplets, the components recognize individual nucleotides. This imparts...intracellular pangenesis to the rediscovery of Mendel. Comptes Rendus de l’Académie des Sciences - Series III ...

  20. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...transfer expert who understands the somewhat complicated Material Transfer Agreement process that kits...function. Project team A Project Team is assembled to complete a series of tasks with precisely defined goals...organization on the planet, in which case whoa! Mission accomplished! One example of a mission-based team in academia...
Showing: 641 - 660 of 939 results