We narrowed to 712 results for: STO

1. Plasmids 101: E. coli Strains for Protein Expression

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   Blog Post

   ...purification protocols are designed for soluble, cystosolic protein products, but this is not always achievable...

2. Adeno-associated Viruses (AAVs) for Genome Editing

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   Blog Post

   ...Although the mechanism of incorporation is not well understood, researchers have been using AAV vectors for ...

3. Transferable Skills Guide: Time Management

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   ...final read Browse list of previous depositors from Boston University Contact Dr. Gilmore directly to see ...

4. CRISPR 101: Homology Directed Repair

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   ...single-stranded DNA templates. Jacobi SA, et al. Poster at Keystone Precision Genome Engineering. 2017.  Easi-CRISPR...

5. Hot Biosensors 2022: Year-End Roundup

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   ...10.1021/acssensors.1c02201. LiLac sensor for lactate Stop and smell the flowers - or rather, the lactate! ...

6. Harnessing Bacterial Toxins for Allelic Exchange

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   ...postdoctoral researcher at Harvard. There’s an astounding number of ways to create chromosomal mutations...

7. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   ...envelope plasmid typically encodes the vesicular stomatitis virus G protein (VSV-G), which facilitates viral...

8. Antibodies 101: Introduction to Immunofluorescence

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   ...with IF are immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these terms refer to...

9. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   ...GTPase optogenetic systems (Berlew et al., 2022). Stopping traffic with CIB1-Rabs Instead of using an optogenetic...

10. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    ... a split BirA* molecule that can reassemble to restore activity when fused to two interacting proteins...

11. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ...CRISPR has taken the genome engineering world by storm owing to its ease of use and utility in a wide variety...

12. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ...) to name two more. Likewise, there are several store-bought cloning kits available which are all based...

13. How to Negotiate a Successful Lab Start Up Budget

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    ...laboratory research management consultant and coach in Boston, MA. He specializes in assisting research scientists...

14. Technologies Enabled by NanoLuc® Luciferase

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    ...furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp to a compact powerhouse...

15. Viral Vectors 101: AAV Variables That Matter

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    ...Kügler, S. (2017). A MicroRNA124 Target Sequence Restores Astrocyte Specificity of gfaABC1D-Driven Transgene...

16. Is this the right place for me? 8 tactics for choosing a lab

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    ...Symposium and #MeTooSTEM, academic bullies are hard to stop—they often have no boss or management structure ...

17. Generating Mouse Models Using CRISPR/Cas9

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    ...Humanization (25-kbp) Created Using Embryonic Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches. Sci Rep...

18. PiggyBac-ing Through the Genome Editing Field

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    ...re-cutting. Transposase removal of the cassette later restores the target sequence. This allows researchers to...

19. Plasmids 101: Restriction Cloning

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    ...to covalently link the backbone and insert and, PRESTO, you have a full plasmid ready to be used in your...

20. Live and Let Dye: Self-Labeling Protein Tags

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    ...., Butler, R., Bewersdorf, J., Rothman, J. E., Johnston, D. S., Schepartz, A., & Toomre, D. (2019). Labeling...