We narrowed to 729 results for: STO

1. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   ...the entry of pathogens to the CNS, but this also stops viral vectors, such as adeno-associated virus (AAV...

2. New Tools Enable CRISPRa for Neuroscience Applications

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   ... visualizing neuronal differentiation by immunohistochemistry, it is not amenable to FACS.  Thus, the ...

3. Lab to Office Culture Shock

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   ...cities around the world, such as BosLab here in Boston. Organizations like this one are always looking...

4. CRISPR-mediated Plant Base Editors

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   ... this mutation by an adenine base editor would restore antibiotic resistance. To find such a protein, ...

5. Using Video to Share Your Science: We Share Science

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   ...is not hard either. Here are some resources: ImpactStory, a website that helps researchers go beyond citation...

6. Changing Labor Laws Bring Increased Postdoc Wages

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   ... all already have salaries at or above $50,000. Boston Children’s Hospital is the only part of the Harvard...

7. CRISPR Activation: A Practical Guide

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   ...plastic tool and has taken the scientific world by storm. While Cas9 has been most widely used to create ...

8. Important Considerations in Optogenetics Behavioral Experiments

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   ...reinforcement are ICSS and place preference.   Historically, ICSS was done by implanting an electrode and...

9. Plasmids 101: E. coli Strains for Protein Expression

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   ...purification protocols are designed for soluble, cystosolic protein products, but this is not always achievable...

10. Lentiviral Vector Guide

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    ...VSV-G due to wide infectivity) VSV-G (vesicular stomatitis virus G protein) is the most common envelope ...supernatant containing the viral vectors is removed and stored or centrifuged to concentrate. You can use crude...line protocol . Browse Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide...on addgene.org Lentiviral Plasmids Collection In-Stock Lentiviral Preps Addgene's Viral Prep Service Viral... The viral envelope gene; typically vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein...

11. Hot Biosensors 2022: Year-End Roundup

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    ...10.1021/acssensors.1c02201. LiLac sensor for lactate Stop and smell the flowers - or rather, the lactate! ...

12. Antibodies 101: Introduction to Immunofluorescence

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    ...with IF are immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these terms refer to...

13. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    ...envelope plasmid typically encodes the vesicular stomatitis virus G protein (VSV-G), which facilitates viral...

14. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    ...GTPase optogenetic systems (Berlew et al., 2022). Stopping traffic with CIB1-Rabs Instead of using an optogenetic...

15. Adeno-associated Viruses (AAVs) for Genome Editing

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    ...Although the mechanism of incorporation is not well understood, researchers have been using AAV vectors for ...

16. Transferable Skills Guide: Time Management

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    ...final read Browse list of previous depositors from Boston University Contact Dr. Gilmore directly to see ...

17. CRISPR 101: Homology Directed Repair

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    ...single-stranded DNA templates. Jacobi SA, et al. Poster at Keystone Precision Genome Engineering. 2017.  Easi-CRISPR...

18. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    ... a split BirA* molecule that can reassemble to restore activity when fused to two interacting proteins...

19. Harnessing Bacterial Toxins for Allelic Exchange

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    ...postdoctoral researcher at Harvard. There’s an astounding number of ways to create chromosomal mutations...

20. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ...CRISPR has taken the genome engineering world by storm owing to its ease of use and utility in a wide variety...