We narrowed to 731 results for: Sis;
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TypeBlog Post...integration within the target cells, ensuring more consistent and reproducible results in calcium assays. There...
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Viral Vectors 101: Virus Safety
TypeBlog Post...ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic... -
Design Tips for Prime Editing
TypeBlog Post...a prime editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer ... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...Shah's and Jim Well's lab induces cre-dependent apoptosis. Now available as a ready to use viral prep in... -
6 Tips for Grant Writing
TypeBlog Post...proof-of-principal study were very helpful in assisting me with applications for much larger fellowships... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post...access to rich and complex microbiome data for analysis. And, well, cats... everyone knows the internet... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post...literature review, survived technical feasibility analysis, and was a marketable product. It was a small ... -
Transferable Skills Guide: Managing a Team
TypeBlog Post... happening even though I met with X on a weekly basis! The silence and maybe even the slight awkwardness... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...a prime editing guide RNA (pegRNA). The pegRNA consists of a target-complementary spacer, a scaffold sequence... -
Pooled Library Amplifications
TypeBlog Post...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog Post...available to immunologists, allowing for the rapid analysis of cell populations within a heterogenous tissue... -
Common Injection Routes in Mice
TypeBlog Post... & Lopez-Berestein, G. (2020). A retrospective analysis for different routes of administration in Mice-Percutaneous... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...inside insect cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus... -
Of Myc and Men
TypeBlog Post...explore Myc’s role in cell proliferation and tumorigenesis. Using two different synthetic peptides corresponding... -
PhD Applications After COVID
TypeBlog Post...in-person events change seemingly daily (and are inconsistent between universities!) This guide tells you ... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...., 2000). Chromoproteins also enhance corals’ resistance to mass bleaching, the loss of their symbiotic... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post.... An imperfect solution is to restrict imaging analysis to cells expressing the lowest levels of the fusion... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...is shipped. Copy number (if known), antibiotic resistance, and optimal growth temperature are listed, as... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor at the University of Miami Miller School...