We narrowed to 738 results for: Sis;

1. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   ...ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic...

2. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

   Type

   Blog Post

   ...Xiong Z, Yang J, Xu Z, Huang Z (2017) Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein...

3. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   ...: Create promoters with the help of epigenetic analysis and designed using stretches of DNA that were ...

4. 6 Tips for Grant Writing

   Type

   Blog Post

   ...proof-of-principal study were very helpful in assisting me with applications for much larger fellowships...

5. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   ...contribute to our understanding of diseases and even assist in drug discovery. References 1. Baird, Geoffrey...

6. Engaging with science and society at pgEd

   Type

   Blog Post

   ...rapidly changing landscape of genetics and our consistent stream of events mean that there is never a dull...

7. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   ...PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic Biology with ...

8. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

   Type

   Blog Post

   ... codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes...

9. Mouse Modeling, Part 1: Genetically Engineered Mice

   Type

   Blog Post

   ...positive selection marker such as an antibiotic resistance gene is also included to select for successfully...

10. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    ...Shah's and Jim Well's lab induces cre-dependent apoptosis. Now available as a ready to use viral prep in...

11. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    ...backbones expressing blasticidin or puromycin resistance genes.  It’s that simple! Good luck and happy...

12. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    ...goal to develop low cost and quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020). To...

13. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    ...access to rich and complex microbiome data for analysis. And, well, cats... everyone knows the internet...

14. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type

    Blog Post

    ...literature review, survived technical feasibility analysis, and was a marketable product. It was a small ...

15. Common Injection Routes in Mice

    Type

    Blog Post

    ... & Lopez-Berestein, G. (2020). A retrospective analysis for different routes of administration in Mice-Percutaneous...

16. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    ...inside insect cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus...

17. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    ...a prime editing guide RNA (pegRNA). The pegRNA consists of a target-complementary spacer, a scaffold sequence...

18. Pooled Library Amplifications

    Type

    Blog Post

    ...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

19. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    ...available to immunologists, allowing for the rapid analysis of cell populations within a heterogenous tissue...

20. Of Myc and Men

    Type

    Blog Post

    ...explore Myc’s role in cell proliferation and tumorigenesis.  Using two different synthetic peptides corresponding...