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We narrowed to 852 results for: Tat

Showing: 641 - 660 of 852 results
  1. Transferable Skills Guide: Creativity

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    Blog Post
    ...: creativity. I’ve heard quite a few scientists state that they’re not creative, usually saying that they...your break can be as simple as a few minutes of meditation or a walk to the break room for a sip of water...
  2. Antibodies 101: Reading a Flow Plot

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    Blog Post
    ...amount of marker that is present, which would be annotated like so: CD14high or CD14low.  Don’t be intimated...liver and kidney recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23...
  3. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post
    ... E. coli B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized...strain library with different gene dosages to facilitate swift optimization of strains. Multiple induction...
  4. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post
    ...Medicago truncatula and several crop species including potato, corn, tomato, wheat, mushroom, and rice (Khatodia...Lew TTS, Sweeney CJ, Koman VB, Wong MH, Bohmert-Tatarev K, Snell KD, Seo JS, Chua N-H, Strano MS (2019)...
  5. Plasmids 101: Biotinylation

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    Blog Post
    ... in the lab are protein purification and immunoprecipitation experiments. Biotinylated proteins or complexes...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...
  6. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post
    ...the architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.”...are using secondaries with fluorophores whose excitation/emission spectra do not overlap. Multiplexed ...
  7. Hot Plasmids: February 2026

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    Blog Post
    ... TimeVault system for preserving transcriptomic state An engineered bacterial strain with a single stop... both lysate and living cells, so transcriptome states can be recorded with cell identity preserved. The...
  8. Filming Science Videos in the Age of Social Distancing

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    Blog Post
    ...zoom on a camera lens without having to physically rotate the rings around the lens. It allowed me to focus... 4).  Learn: This works great for on-camera presentations and interviews. But how can I adapt this setup...
  9. Plasmids 101: Dimers and Multimers

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    Blog Post
    ...the transformed bacterial culture into the late stationary phase. Growing the culture at a lower temperature...low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol Bioeng. 2009 Aug 15;103...
  10. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post
    ...AAV receptor (AAVR)2 Laminin receptor (LR)3 Putative integrin3 AAV11 Carboxypeptidase D (CPD).... (2006). Α2,3 and α2,6 N-Linked Sialic Acids Facilitate Efficient Binding and Transduction by Adeno-Associated...
  11. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post
    ... signal from low abundance targets. Analysis/Presentation Once you’ve captured your images, various software...each cell. When making figures for a paper or presentation, make sure the images are accessible to all ...
  12. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post
    ...gene is also useful in case the gene has a weird mutation that you didn’t know about (which could affect...theory you could use densitometric analysis to quantitate the intensity of the bands on your gel and get...
  13. Custom CRISPR Screens & the Green Listed Software

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    Blog Post
    ...cells used and the readout, this could be a major limitation. An alternative to the full genome CRISPR screen...PhD student. In particular, one slide of the presentation made a lasting impression on me. The slide showed...
  14. Typing CRISPR Systems

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    Blog Post
    ...resembles cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2, which are critical for ... become the most engineered Cas enzyme, with adaptations for increased fidelity, activation/repression...
  15. CRISPR 101: Cytosine and Adenine Base Editors

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    Blog Post
    ...being able to specify and correct single-base mutations without introducing DSBs at all. Base editing ...are smaller and less likely to cause off-target mutations than those derived from naturally occurring cytidine...
  16. Troubleshooting and Optimizing a Western Blot

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    Blog Post
    ...lane 1 is‌ lane 8. Common ways to keep membrane orientation straight are to mark the upper left-hand corner... Ensuring wash is happening under sufficient agitation (rocking) Add Tween-20 to wash buffer (can...
Showing: 641 - 660 of 852 results