We narrowed to 954 results for: Tes

...a blank white slate and developed this over 30 minutes, which was fun to watch. A simpler 1 protein -... is already modifying cell and animal genomes to test hypotheses of disease. They are finding they have...

...until now, you’ve always had to call the United States - Cambridge, MA to be exact. Today, that changes...expression of an unknown protein in macrophages/monocytes using lentiviral expression vectors. I also used...

...: Periodic surveying of cell line collections websites and catalogues Literature searches utilizing Google...correct one!)    Fig. 1: HeLa cells. Image courtesy of Josef Reischig.  Contributing to Cellosaurus...

...of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...recommended for use in western blot, but we are actively testing it in other applications! If you try it out in ...

...a conformational change of the receptor which modulates the fluorescence intensity of the cpGFP without... vitro, and they further demonstrated that when tested in tobacco protoplasts, the enzymatic biosensor...

...repository  Name Serotype Category Depositor  Notes AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

...with the article metadata, legend and user created notes on a “Pinterest for scientists”? ReFigure extension...profile. To see how to create ReFigures in a few minutes, visit our YouTube Channel. Currently we can only...

... four new plasmid pages that cover some of the hottest CRISPR tools. Base Edit: Base editing plasmids...Plan Your Experiment. #12DaysofCRISPR and blog updates We hope you enjoyed the #12DaysofCRISPR, a celebration...

... to the molecule it detects. This change then generates a reporter signal. Reporter signals may be electrochemical...Biosensor Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays...

...microbiology with multiple high-profile publications. My classmates consisted of graduate students, post-grads, and...manuscript is like generating a quilt with your lab mates, you add the individual figures and results to make...

...pages, performance data, protocols, and even certificates of analysis.” Han’s biggest piece of advice... at eScience Labs, an educational company that creates hands-on science lab kits and digital curriculum...

...requested from Addgene many times as the technology disseminates throughout the research community. These popular...level system on our website:   A yellow flame indicates that a plasmid has been requested 20+ times   ...

...stayed to see the Opening Keynote address with Bill Gates. The long line for bag check was definitely worth...for global health and the various initiatives the Gates’ Foundation is involved with was one of the highlights...

... these meetings in relatively isolated resorts creates a sense of camaraderie with fellow attendees from...tackling this type of genetic engineering is a testament to how amazing the downstream payoff could be....

...DNA for 2 minutes at 72°C. Repeat steps 8-10 for 25-30 cycles. Final Extension for 5 minutes at 72°C. ...oligo primers. This molecular biology technique creates several micrograms of target DNA from just a few.... Basic PCR Program Initial Denaturation for 2 minutes at 94°C: This initiation step heats the double ...Repeat steps 2-4 25-30 times. Final Extension for 5 minutes at 72°C: A final extension to fill-in any protruding...melting temperature (Tm). Set extension step at 1-2 minutes per kilobase of product depending on whether you...time and temperatures. Initial Denaturation for 2 minutes at 94°C. Denature for 30 seconds at 94°C. Anneal...denaturation of the DNA, particularly for GC-rich templates. What does each ingredient specifically do? Template...

...After a virion infects a cell, the single particle creates many copies of itself, sometimes up to millions...infected cell.) This is in contrast to bacteria and eukaryotes, which use a binary replication process whereby...

...again until you don’t.” As every researcher can attest to, science is nothing if not solving your way ...creativity, changed the outcome of the project, or illustrates the value of the work and then build a story ...

...applied for identification of essential MYC binding sites in a wide range of human cells.  A new pooled library...targeting start codons, splice acceptor and donor sites, and introducing premature stop codons.    New...

...repository  Name Serotype Category Depositor  Notes pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

...KSQ uses a CRISPR-Cas9 platform to systematically test the function of all 20,000 genes in disease models... of Oncology Target Discovery at the Novartis Institutes for Biomedical Research. Drug development was...