... first need to decide the best way to track or measure the process you’re interested in. Depending on ...examine, i.e. for an enzymatic reaction, you could measure consumption of a substrate OR production of a product...molecule biosensors Addgene has many sensors that measure the concentration of small molecules like arabinose...withstand a particular solute concentration or temperature? For further advice, see our posts on how to ...

...very painful FOP flare ups. Eventually, without a cure, AJ will begin losing mobility and not be able to...play like the other kids his age. A potential FOP cure But, there is HOPE. FOP is a genetic disease typically...solutions to treat rare diseases like FOP until a cure is available. Addgene and AJ's Avengers This year...knowledge of FOP, it’s biological causes, and potential cures. We encourage any researchers working on ACVR1 or...

...boxes). We get these boxes a lot when receiving temperature-controlled reagents. Expanded polystyrene will...company and it has to be packaging (no coated tissue culture plates, for example). Check the link for more details...or Axygen®, set up a bin or box in your tissue culture room for the wrappers and ship them back. Start...make a huge impact directly on waste, but also on culture. Encourage your workplace to become a leader in...

... of the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes the... the method is powerful and offers immediate measurements in a live animal that can be correlated to behavior...developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine sensor In 2018, Lin Tian...powerful new developments in the detection and measurement of neurotransmitters in various species and while...

...young scholars across Europe get introduced to the cultures and opportunities available to them at university...to students. Freshers fairs are also where many future scientists are introduced to the biology and chemistry...talks by prominent scientists, including Nobel Laureates, who speak about their work and current, trending...positions both inside and outside academia. Inspiring future scientists At Addgene, we’re incredibly glad to...

...readthrough mitigation. Nature. (2016) 534, 719-723. DOI: 10.1038/nature18308   Additional resources on...are unreliable for a variety of reasons (Baker, Nature). Tagging your protein allows you access to a ...Reproducibility crisis: Blame it on the antibodies. Nature (2015) 521, 274-276 DOI: 10.1038/521274a Arribere...

... both targets in your specimen. You scour the literature and find an ideal antibody against Protein A ...distinguish between your two targets.    Figure 1: In the isotype conversion process the variable...successfully visualize both proteins.     Figure 2: Two primary antibodies with the same isotype... the anti-Protein B antibody (Fig. 3)    Figure 3: Recommended secondary antibodies for rat IgG1...

...synthesis. They published their results in a paper in Nature Communications announcing a new bioinformatics ...good predictors because they are just that: rare. “Nature has many genes where there are not rare codons ...source genes from farther away in the tree of life." Nature communications 9.1 (2018): 4425. PubMed PMID: 30356044...to predict the lab-of-origin of engineered DNA." Nature communications 9.1 (2018): 3135. PubMed PMID: 30087331...

...Updated April 21, 2020 Picture this: you’re a busy scientist anxiously awaiting the arrival of the newest...signs each agreement just once, covering nearly all future requests/deposits. This reduces the processing ...between a TTO and Addgene, whose terms apply to all future deposits of materials with Addgene Master Reception...between a TTO and Addgene, whose terms apply to all future materials requests from Addgene MTA: material transfer...

...selection-based gene tagging approach Multiple features of C. elegans have made CRISPR/Cas9 so successful... sequences are inserted into the genome. Figure 1: Self-excising cassette schematic from Dickinson...efficient for C. elegans genome modification. Figure 2: Schematic from Dickinson et al. (2015) illustrating...may prove effective in other species or in cell culture. In addition to CRISPR/Cas9 applications, SEC selection...

... possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch sensor...by a secondary screen in rat hippocampal neuron cultures. The optimized Twitch sensors allowed the researchers...toxic,” Merkx explained. New tools were needed to measure them. Merkx says, while in principle adapting a...them,” Merkx said. “For us, it is one of the main measures of success – whether we have really developed ...

...CRISPR screening.   Using tRNAs Luckily, a fun feature of tRNA was fully unleashed by the Gao Lab’s work... for gene editing. They named this new array architecture the DAP array, short for “drive-and-process....payloads. Different from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely...Comparison of previous representative dual-gRNA architectures with DAP array   Qichen Yuan is a PhD...

..., analyzed your data, and made your tables and figures. Now it's time to write. Sometimes, it's hard to...Linux.  Mendeley and Zotero have a lot of the same features, but this chart highlights the differences between...Can Grad School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear Grad Student...blog: Tips for writing scientific manuscripts: literature searching, reading, and organizing Tips for writing...

...participants could share their notes together. Figure 1: Can you draw Spongebob from these instructions...visuals, or asking questions using the polling feature or the chat box. One key thing to remember regardless...getting to know the app or virtual platform and making sure you stay connected. If possible, test out the technology...connection,” says Abitua about her workshop. “We made sure that we had a backup plan in case someone’s internet...

...course (read our blog post on it and to see old pictures of the lab!). By 2016, we wanted to be taken seriously...working in our laboratory! Don’t worry though, we make sure that everyone gets proper equipment and safety training...project at BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge...yeast transformations! Dennis Oleksyuk, Raphael Laurenceau, and Tami Pudina have initiated a soil microbiome...

...problem or complete a project, overcoming inevitable failures and setbacks. There are many roles that actively...because they enjoy spending time outdoors and with nature. Experience in the field or in a position that ...Conservationist Documentary Director Parasitology Botanist Nature Photographer/Artist Ecologist     Computer...Include any relevant job specific ‘buzz words’ to ensure recruitment agencies and employers put your resume...

...serotonergic neurons (measured using a red calcium sensor) leads to the release of serotonin (measured using the ...throughput manner. She helped develop a method that measures the effects of sequence variants on abundance ...metabolism of leaves can be changed by light and temperature and leaf autofluorescence can interfere with ...

...use agreements like MTAs.  Addgene does a lot to ensure that we can facilitate a fast and agile MTA process...material under the specified terms. This acts as the signature on any subsequent MTA for that plasmid facilitated... handled the majority of our MTA requests and signatures via an online process, though the process has...materials are requested, instead of coordinating signatures from two different institutes. Our online portal...

...actually mean? What relevance does it have on the structure of a bacteria species and how it can be used in...differences in cell wall composition. Cell wall structure Gram positive bacteria, or monoderms, have a single...the gram negative bacteria’s thinner membrane. Procedure The stain is a pretty simple process. First, flood... them red or pink. At the end of the staining procedure, gram positive bacteria are stained purple or ...

...and with a group who all strive for a common big-picture goal. It is worth doing some some serious pre-work...position. Am I choosing the right mentor? Make sure your advisors will be good role models and teach... career path?  If you are considering this, make sure to choose an advisor that supports both paths – ...details and many more suggestions on planning for future success by finding and joining your dream lab, ...