...different incubation options available. Caution Use the Stop Solution in a well ventilated area and avoid ...Immediately before use, mix 5 mL TMB Solution and 5 mL Peroxide Solution from the TMB substrate kit. Cap the...determine the ideal concentration. Prepare serial dilutions of the purified antigen as follows: 2 ng/µL : ... wells of each row, using a different standard solution for each row of a 96-well ELISA microplate. Cover...the wash buffer from the wells. Prepare antibody dilution buffer (0.1% BSA in PBS) as follows: Dilute 50... to the desired concentrations in the antibody dilution buffer. Pro-Tip The ideal antibody concentration...multichannel pipette, add 100 µL of primary antibody solution to the appropriate wells. Cover the plate with...

...Option #1 - Solution D Protocol Before starting this protocol, make sure to prepare solution D (see reagent...or lyse tissues or cells in Solution D. For tissues: use 1 mL of Solution D per 100 mg of cells. For cultured...resuspension. This protocol provides two options: using Solution D (see reagent section for recipe) or using an...all-in-one acid guanidinium thiocyanate-phenol solution such as TRIzol®. For the precipitation step, two...reagents RNase-free (Use an RNase decontamination solution, such as RNaseZap® or RNase AWAY®, may be used... Vortexer -20°C freezer -80°C freezer Reagents Solution D (for Protocol Option #1 ): 4 M Guanidinium Thiocyanate...alcohol (49:1) 75% Ethanol RNase-free water or TE solution RNase free tubes: microcentrifuge tubes, 4 mL ...

...viral dilution to each well (each well gets one dilution, so a 6-well plate will hold 5 dilutions plus ...just a sample of possible dilutions. You may want to try higher/lower dilutions depending on your downstream...the results of your titration. Mix the dilutions well. Dilution Volume of Lentivirus (μL) Volume of DMEM...that already contain 0.5 mL of virus solutions at various dilution. Make sure to use the polybrene-containing...batch of DMEM complete + 10 µg/mL polybrene by diluting 20 µL of 10 mg/mL polybrene into 20 mL media. ...aliquot on ice prior to use. Prepare a range of dilutions of the lentivirus in DMEM complete + 10 µg/mL ...polybrene-containing media to make the cell solution in this step. To seed the cells: Prepare a batch of cells as ...

...compounds, as circulation is slower, and more institutional oversight is required. Restraint: mechanical...

...means being someone’s boss. But it does not mean shouting orders and trying to get everything done your ...

...working with Jane Richardson, provided an initial solution to this problem with the development of Byron’...

... catch all of these potential mishaps before distributing the vectors? Viral genome sequencing Thanks ...

.... Furthermore, one must bear in mind that the resolution of light microscopy is limited by the wavelength...

..., AID has gone through an additional round of evolution (AID-2) to reduce some of the leakiness associated...

...Chemigenetic sensors take a hybrid approach and utilize both a genetic component as well as a small molecule...

...facility which can be expensive to gain access to routinely, and it can be especially challenging when trying...

...kinda. In some cases, antibody providers are distributing monoclonal antibodies derived from the same ...

...molecular diagnostics.       Steps of PCR The beauty of PCR is that it can amplify DNA using only a ...saving both time and effort. Scientists can also utilize multiplex qPCR to quantify the concentrations of...

... virus may acquire mutations to escape these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer...

...November 18, 2016): Researchers from a variety of institutions recently reported their inability to recapitulate...

...control of a rhamnose-inducible promoter. The resolution step leads to the desired mutation and excision...

...wall trying to study it? It happens. Is it an evolutionarily conserved gene? Can you find an ortholog in...

... was assembled, and enables requesting labs to utilize and modify the plasmid in their own lab.  If depositors...

...vector Promoter driving Cas9 expression can be constitutive or inducible. U6 promoter is typically used ...

...swirling the flask occasionally as the solution heats up.). Caution HOT! Be careful stirring, eruptive boiling...do you get better resolution of bands? A few simple ways to increase the resolution (crispness) of your...gel electrophoresis, including tips to improve resolution and separation of bands. Protocols... completely dissolved (but do not overboil the solution, as some of the buffer will evaporate and thus...continue towards a boil. Keep an eye on it the solution has a tendancy to boil over. Placing saran wrap...necessary if you pay close attention. Let agarose solution cool down to about 50 °C (about when you can comfortably...0.2-0.5 μg/mL (usually about 2-3 μl of lab stock solution per 100 mL gel). EtBr binds to the DNA and allows...