Making CRISPR Plasmids Using Fragmid
Type
Blog Post
...Mammalian
Transposon
pRDA_757 (Piggybac; constitutive expression)
Mammalian
Plasmid
pRDA_791...
We narrowed to 750 results for: Uty
-
-
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...discoveries. You’ve analyzed the data and generated beautiful figures. And, you can’t wait to tell your story... -
Tips for Technical Support Calls
TypeBlog Post...interaction - one that gives our customers the most utility in the least amount of time. Though I now spend... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...Perhaps most striking is that there were seven institutions involved in the effort. This inclusive and open... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...Multiple copies of the Mason–Pfizer monkey virus constitutive RNA transport element lead to enhanced HIV-1... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post... further reading and viewing The Epigenetics Revolution: How Modern Biology is Rewriting Our Understanding... -
The Materials Science of Optogenetics Experiments
TypeBlog Post... on Twitter @derekpsimonphd. References 1. Gutierrez DV, et al. Optogenetic control of motor coordination... -
Fluorescence Titering Assay
TypeProtocol...will be higher titer, consider additional dilutions. Dilution Volume of Lentivirus Stock (μL) Volume of... Mix the dilutions well Gently aspirate media from the cells. Add 1.5 mL of a viral dilution to each well...multiple dilutions. Sample Data Figure 1: 293T cells were transduced with a range of dilutions of pHAGE-TO-dCas9...ensure that you are in compliance with your institution’s biosafety regulations. Workflow Timeline Day...lentiviral aliquot on ice prior to use. Prepare dilutions of the lentivirus into DMEM complete containing... well (each well gets one dilution with one well remaining as the untransduced control). Count the cells...transduction units per mL (TU/mL) using either the dilution factors (method 1) or the volume of virus (method... -
Transferable Skills Guide: Managing a Team
TypeBlog Post...means being someone’s boss. But it does not mean shouting orders and trying to get everything done your ... -
Common Injection Routes in Mice
TypeBlog Post...compounds, as circulation is slower, and more institutional oversight is required. Restraint: mechanical... -
Plasmids 101: Degron Tags
TypeBlog Post..., AID has gone through an additional round of evolution (AID-2) to reduce some of the leakiness associated... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post...Chemigenetic sensors take a hybrid approach and utilize both a genetic component as well as a small molecule... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...molecular diagnostics. Steps of PCR The beauty of PCR is that it can amplify DNA using only a ...saving both time and effort. Scientists can also utilize multiplex qPCR to quantify the concentrations of... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post... virus may acquire mutations to escape these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...facility which can be expensive to gain access to routinely, and it can be especially challenging when trying... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...control of a rhamnose-inducible promoter. The resolution step leads to the desired mutation and excision... -
Of Myc and Men
TypeBlog Post...kinda. In some cases, antibody providers are distributing monoclonal antibodies derived from the same ... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...working with Jane Richardson, provided an initial solution to this problem with the development of Byron’... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post... catch all of these potential mishaps before distributing the vectors? Viral genome sequencing Thanks ... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post...wall trying to study it? It happens. Is it an evolutionarily conserved gene? Can you find an ortholog in...