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...Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching system that follows the sample from the...

...series will help you find new CRISPR tools for your research.     Improved nucleic acid sequence detection...human genome, these donor plasmids will help researchers map transcription factor localization and binding...

...Chiara DeNatale and programming with our Chief Architect Pam McA' Nulty (check out our blog post on CRISPR... travels with Blugene Blugene loves to visit researchers, so Outreach Scientist Jessica Welch showed some...

...infrastructure. A Stakeholder Workshop at Janelia Research Campus in 2019 solidified the project and the ...Workshop Report Public Release Event scheduled for March 4th from 2:30-4:00 pm ET.  You can learn more about...

...coli Transformation Kit and Buffer Set  from Zymo Research to make competent cells because cells prepared...shock. Detailed protocols are available via Zymo Research. In brief, we grow our E. coli in LB to log phase...

...mRNA binding factors (PUF). In a single step, researchers can now assemble designer PUF domains for RNA...published in The Journal of Biological Engineering in March is fused to a translational repressor. In principle...

...topic can be found on the UK Career Site for Researchers called Vitae. Vitae recently published a career...Download a summary PDF here or try out the online Researcher Development Framework Planner. For More Reading...

...A few years ago, researchers introduced what’s been called a major breakthrough in cloning technology...GreenGate – offers all of those same advantages to researchers working in plant model systems. “The cloning ...

... Medintz, I. L. (2019). FRET as a biomolecular research tool — understanding its potential while avoiding...

...will help you find more new CRISPR tools for your research! This time: CRISPRi in Burkholderia Base editors...lentivirus-like particle. Read the paper in Nucleic Acids Research Find the gRNA and packaging plasmids   Cytosine...

...Addgene Director of Biology, Lianna Swanson, and researcher at Sanofi Pharmaceuticals. This post highlights... Addgene's Cancer Collection Pages Connect the Research Community Grad School Advice Part 1: Picking a...

...you might expect with any nascent technology, researchers are having trouble getting NgAgo genome editing...successes, and to find out what delivery techniques researchers are using. The detailed results of his survey...

...interesting work. Consider this to be like any other research project. You’ve met someone whose career interests...Informational Interview is a great next step in your research. What an informational interview is not If ...

...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot... cells, and it may find wide use in biological research. Photoconvertible fluorescent proteins Newly...

... purification specialists recommend doing some research, notably determining the subcellular localization...for cloning (see schematic below), enabling a researcher to test multiple tags for one protein quickly...

...) to make it easier to know where to start your search. Then identify the most frequently used pages in...checklists that link to other notes, and use the search bar to quickly find what you’re looking for. Tip...

...pAAV-CBh-mKate2-IRES-MCS (WT-WT) CBh mKate2 Constitutive 2 Marcella Patrick 114469 pAAV-CaMKIIa-mCherry CaMKIIa mCherry...

... a scientist). Profiles and Twitter lists were searched for scientific titles generated from the United...Twitter. Or maybe scientist prefer to share links to research that isn’t behind paywalls. 5. The #1 (re)tweeted...

...wealth of genetically encoded tools available, researchers are also developing ways to improve delivery ...launched AAV Data Hub, an open platform where researchers using AAV from our viral service can share experimental...

...representing imaging from 47 different tissues. Researchers upload and share both their successes (recommended...detergents that they used in their experiments. Researchers can then visit the IBEX imaging community discussion...