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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 819 results for: cell

Showing: 641 - 660 of 819 results

  1. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    ...institutions in Ghana and most recently got his PhD in cell and molecular biology at the University of Edinburgh...developing novel systems for bioconversion of cellulosic biomass to useful products under Professor Christopher...biology, DNA assembly methods, and engineering cellulose degradation. 18:21 - 23:26 : Kwabena's plans for...

  2. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... inosine, which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine ...

  5. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...biologically relevant forces at focal adhesions in living cells in several different contexts.   Figure ...substrate furimazine. Right: Demonstration of PILATeS. Cell adhesion to a PILATeS-functionalized coverslip creates...useful addition to the toolkit for visualizing cellular processes in C. elegans: mScarlet3 for applications...

  6. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... be safely used to deliver a genetic payload to cells, and the complexity of effectively deploying them... can be to use. AAVs, which can target different cell types depending on their serotype, are particularly...

  10. Deep Dive: qPCR

    Type
    Blog Post
    ... that your reference genes are suitable for your cell lines and/or tissue types.  Please do note my oh-so-subtle...reference genes to save on plate space. For many cell lines and tissue types, appropriate reference genes...at determining the best options for a particular cell or tissue type (such as here, here, or here).  If...your data  Once you’ve done the work to produce excellent and reliable qPCR data, you’ll of course want ...

  11. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...spatio-temporal control of gene expression within a cell. Thanks to the work of Huimin Zhao and colleagues...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...

  12. E11 Bio PRISM Collection

    Type
    Collection
    ... with the delivery of stochastic combinations of cell-filling antigenically distinct protein tags (or ...

  13. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...are often used to study vertebrate embryology and cell development. One major resource for frog research...Organism to Study and Model Human Genetic Disease. Cells Tissues Organs, 205(5–6), 303–313. https://doi.org...

  16. Technical Design of a Western Blot

    Type
    Blog Post
    ... mechanical method before your enzyme lysis. For cell cultures, enzyme lysis is typically sufficient.  ...proteins easily available in the cytoplasm or whole-cell lysate. A harsher, SDS-containing lysis buffer, ...while semi-dry transfers require a semi-dry transfer cell. Dry/electroblot transfers only work in the proprietary...expensive Nitrocellulose or low-fluorescence PVDF membranes Increased fragility (nitrocellulose) or increased...When choosing your lysis buffer, consider the subcellular location of your protein. Proteins located in...Membranes The two membrane options are PVDF and nitrocellulose. PVDF is a sturdy membrane that can be stripped...membrane, it also has higher background noise than nitrocellulose membranes. Pro tip! You can visualize proteins...

  18. Distribution to Industry

    Type
    Collection
    ...plasmids for detecting GPCR activity in a variety of cell types. Protein Purification Optimization Kit Using...
Showing: 641 - 660 of 819 results