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  4. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...the Addgene website. blog.addgene.org The blog provides tips for working with viruses including advice...on each viral prep’s specific material page and includes western blots as well as other expression data...

  6. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...without end-point sequencing assays.  This tool was designed by putting together the coding sequence of a mCherry...MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent...

  8. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies...

  12. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...if you end up concentrating your AAV above your desired titer and want to dilute it, err on the side of...less buffer than you would to achieve the exact desired concentration to avoid over-diluting, and make ...

  13. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually...

  14. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ... determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing... usually translated in a specific compartment. Despite these limitations, these techniques represent powerful...

  16. Addgene Depositors Get More Citations

    Type
    Blog Post
    ... Science. For each publication, Web of Science provides basic bibliographic information and uses its vast...citations due to the journal of publication. Table 1 provides an example calculation of the weights. Table 1...

  17. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...best serotype for their particular study, which includes comparing the transduction patterns of different...fluorescence of the sensor. This “contrast” variant provides improved detection for tracking large populations...

  18. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... chromosome-specific loci in the genome and the guides are relatively unstable.   Figure 1: CRISPRainbow... the gRNA tetraloop and went through subsequent design iterations to increase stability and minimize misfolding...

  20. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...visiting role is because the host lab or institute provides something that you can’t obtain at your current...skills and equipment! Preparing for your visit   Design and communicate a research plan Your time at the...
Showing: 641 - 660 of 983 results