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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 986 results for: des

Showing: 641 - 660 of 986 results

  1. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ... determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing... usually translated in a specific compartment. Despite these limitations, these techniques represent powerful...

  3. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...some point. Usually it comes up when a researcher decides on a new project and begins by trying to reproduce...they really want to do is get the paper off their desk and move on. Nevertheless, editors can and do play...

  4. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...best serotype for their particular study, which includes comparing the transduction patterns of different...fluorescence of the sensor. This “contrast” variant provides improved detection for tracking large populations...

  5. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...The natural CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to ...applications, we provide detailed manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715...

  6. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...visiting role is because the host lab or institute provides something that you can’t obtain at your current...skills and equipment! Preparing for your visit   Design and communicate a research plan Your time at the...

  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...biomass production techniques in the following decades, Phillips made the decision to release the K. phaffii...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...

  8. Hot Plasmids - January 2023

    Type
    Blog Post
    ...effects, both of happen with Cas13. CRISPR-Csm degrades both nuclear and cytoplasmic mRNA with 90-99% ...crystalizing the hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you...

  9. Addgene Depositors Get More Citations

    Type
    Blog Post
    ... Science. For each publication, Web of Science provides basic bibliographic information and uses its vast...citations due to the journal of publication. Table 1 provides an example calculation of the weights. Table 1...

  10. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...affordable. 4. Stem cell scientists are generous Besides some of the scientists I've already mentioned, ...

  11. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...mammalian plasmid components, it may be useful to describe the means of introducing genetic material (such...the plasmid in the same way. Selection methods, described below, are typically employed when creating stable...

  12. Antibodies 101: Isotypes

    Type
    Blog Post
    ...molecules are recruited by the antibody to help destroy and remove a pathogen. Different isotypes also ...well as the genes that encode those proteins are designated by the lower-case Greek letters μ, δ, γ, ε, and...

  13. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ... such as citizenship status, academic year, and grades that serve as automated filters of consideration...and cover letter revised by your network, which includes your friends, family members, and professional...

  15. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...induction of a promoter. Below, you’ll find a short description of these three types of inducible promoters, ... popular light-regulated two component systems designed by the Tabor lab. Which inducible promoter is ...

  17. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top of very high coverage....researchers have used the flexibility of AAV genome design in remarkably creative ways. The continued development...

  19. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...familiar with the basics of controls in experimental design, you’ll need a few controls specific to flow as...controls like FMO or isotype controls, which we’ll describe a bit later. Single Colors Single color controls...

  20. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...promoter should flank this component as well. On both sides of this promoter-gene combo are two long terminal...Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from: https://www.ncbi.nlm.nih.gov/books...
Showing: 641 - 660 of 986 results