We narrowed to 960 results for: form

1. Hot Plasmids and Viral Preps - July 2021

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   ...the Rawlins Lab have compiled a new toolkit for performing genetics studies in organoids. The Organoid EasyTag...petunia SL enzymatic receptor DAD2. SLs induce a conformational change of the receptor which modulates the ...

2. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

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   ...goes on our website. We look at product pages, performance data, protocols, and even certificates of analysis...Addgene Blog Get tips on how to do your own informational interview Check out this post if you debating...

3. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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   ...fulfill its mission of helping scientists share information and, of course, plasmids. My primary duties are...presenting, analytical thinking, time management, information distillation, etc) are transferable to many different...

4. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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   ...isolated from Eubacterium rectale, significantly outperforms other RTs in this regard and is even able to ...antibodies. These properties make nanobodies easier to transform and express in bacteria, which, in turn, makes...

5. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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   ...help spot inconsistencies in the work, missing information that would prevent the study to be replicated...comments to “you” I can easily check if I would have formulated specific comment differently if I would be speaking...

6. Fluorescent Biosensors for Measuring Autophagic Flux

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   ...a static measurement and it provides limited information about what materials or organelles are being ... acidic pH found in lysosomes. Therefore, the formation of autophagosomes leads to an increase of yellow...

7. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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   ...viral service but did you know that Addgene also performs independent research? Since launching the viral...sorbitol is already a common excipient in AAV formulation buffers, this sugar may be a more attractive ...

8. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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   ...choosing a genomic target. NgAgo also appears to outperform Cas9 in GC-rich regions. DNA guides NgAgo uses...re looking to disrupt gene function via indel formation, this property could be an advantage. However,...

9. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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   ...pSC102, with EcoRI and selected for E. coli transformants that were resistant to both. When this proved... Central PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal...

10. "Build Your Plasmid": The Game - Play to Teach

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    ...but it can be difficult to determine how much information they’ve absorbed if they don’t get a chance apply...gaps in their knowledge. Practically speaking the format I decided on was to have two teams of 4 people ...

11. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    ...together and force the strands into a parallel conformation. Between β11 and one of the linkers is a consensus...-11, where β10-11 was forced into a parallel conformation by the E5 and K5 linkers that are connected ...

12. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    ...and the pH shift can be prevented. Keep this information in mind if you’re planning on shipping something...study found that dry ice storage affected the performance of antibodies, because of effects of pH on protein...

13. Addgene Depositors Get More Citations

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    ... Web of Science provides basic bibliographic information and uses its vast network of articles to track...including biotech and research tools, computing performance, and randomized control trials in innovation....

14. Fluorescent Proteins 101: Luciferase

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    ...Renilla luciferase from the sea pansy (Renilla reniformis) emits a blue light with a peak emission at 480nm...Vargulin) luciferin None Yes Sea pansy(Renilla reniformis) 480 36 Coelenterazine None No Deep sea coceopod...

15. Developing a cold tolerant E. coli using specialized chaperones

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    ... host cells (the simplest example would be a transformed mesophilic, for example E. coli, carrying a plasmid...2019 All-India iGEM Meet-up, which served as a platform for all the Indian iGEM teams to interact with...

16. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    ...identify Cas13d, both groups used very general bioinformatic screens that looked for a CRISPR repeat array...of Type VI Cas13d enzymes is another win for bioinformatic data mining. As we continue to harness the natural...

17. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    ...application of blue light, iLID undergoes a conformational change allowing high affinity binding to SspB...These optical reporters have limited deep-tissue performance, and sometimes require an alternative such as...

18. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    ... an antibiotic resistance gene to select for transformed bacteria, but you might be wondering, “How do...References 1. J. R. Bateman, et al. Site-Specific Transformation of Drosophila via PhiC31 Integrase-Mediated...

19. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    ... UCSF Immunoprofiler - a human biopsy analysis platform focused on analysing immune measures and correlating...with tumor mutations and clinical responses. This platform has the potential to become an open-source pipeline...

20. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    ...decision for each patient if the majority of information on each cancer clinical sequencing report includes...publications), it will be possible for many others to perform additional functional assays for these mutations...