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...fluorescently labeled piggyBac vectors to deliver uniform and sustained expression of multiplexed gRNAs in... bloggers. Check out this blog post for more information. Additional resources on the Addgene blog ...

...taking info from the code of viruses to give information to the computer to fight the known viruses. Bacteria.... As Dr. Mello said, “Cells have been in the information age for billions of years (…) Bacteria are really...

...of plasmids is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent Protein...fluorescent proteins are concerned.” For more information on choosing a backbone and making fusions, see...

...scientific outreach and community engagement. Information about contacting Addgene can be found on our ...struggled to get those plasmids with the right information and I believe that a lot of European students...

..., we ensured that access to reliable plasmid information keeps pace. Opening our plasmid data through ...AGO4 gene against rice hoja blanca virus: from transformation to protein structure. Frontiers in plant science...

...goes on our website. We look at product pages, performance data, protocols, and even certificates of analysis...Addgene Blog Get tips on how to do your own informational interview Check out this post if you debating...

...fluid (Darvesh et al., 2011). This technique is performed with a probe surrounded by a semipermeable membrane...The advantage of this method is that it can be performed in a live awake animal by surgical implantation...

...react by performing a distinct C-bend to escape possible danger (see figure). Larvae also perform spontaneous...

...vector containing the newly inserted gene is transformed into bacterial cells and selectively grown on...Addgene’s Molecular Biology Reference includes information about molecular cloning, how to choose a plasmid...

...the Rawlins Lab have compiled a new toolkit for performing genetics studies in organoids. The Organoid EasyTag...petunia SL enzymatic receptor DAD2. SLs induce a conformational change of the receptor which modulates the ...

... they could solve this problem. D10A nickase outperformed both WT Cas9 sites tested by a wide margin, ...use Cas9 nickases in genome editing. For more information and a link to the archived webinar, click here...

...that much tissue takes a week of multiple people performing dissections. With CUT&RUN, we have had success...up until this point. The nuclease reaction is performed on ice, and only for a short period of time, thus...

...Some use cases include: Cataloging a reagent’s performance as in this antibody ReFigure Analyzing replications...confirmatory image to show that a previously used reagent performs well, or you might publish a replication experiment...

...fulfill its mission of helping scientists share information and, of course, plasmids. My primary duties are...presenting, analytical thinking, time management, information distillation, etc) are transferable to many different...

...isolated from Eubacterium rectale, significantly outperforms other RTs in this regard and is even able to ...antibodies. These properties make nanobodies easier to transform and express in bacteria, which, in turn, makes...

...help spot inconsistencies in the work, missing information that would prevent the study to be replicated...comments to “you” I can easily check if I would have formulated specific comment differently if I would be speaking...

...a static measurement and it provides limited information about what materials or organelles are being ... acidic pH found in lysosomes. Therefore, the formation of autophagosomes leads to an increase of yellow...

...viral service but did you know that Addgene also performs independent research? Since launching the viral...sorbitol is already a common excipient in AAV formulation buffers, this sugar may be a more attractive ...

...choosing a genomic target. NgAgo also appears to outperform Cas9 in GC-rich regions. DNA guides NgAgo uses...re looking to disrupt gene function via indel formation, this property could be an advantage. However,...

...pSC102, with EcoRI and selected for E. coli transformants that were resistant to both. When this proved... Central PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal...