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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 938 results for: mpl

Showing: 641 - 660 of 938 results

  1. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...to be managing. Being reminded of the seemingly simple actions we can take to help ourselves can be an... in the family to have to miss something – for example, say no to some of those birthday parties. Tell...time. For me, that’s been volunteer work at our temple, my kids’ school, and with professional organizations...

  2. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...also operates with a single RuvC-like domain. In simple terms, Cas9 cuts DNA using two "scissors" (one ...a 20-nucleotide (nt) guide sequence that is complementary to the DNA target. The 20-nt guide region binds...Oligo 2: Add 'ggcc' to the 5′ end of your reverse complement guide (see Figure 1). Perform standard phosphorylation...

  3. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...pBAD-bARGSer-AxeTxe, gas vesicle expression in E. coli is easy: simply grow cells in Lennox LB (10 g/L tryptone, 5 g/L...improved purity and contrast. For in vivo experiments, simply induce injected engineered cells via regular intraperitoneal...a chemical transfection reagent (e.g. PEI-MAX). Simply cotransfect a mixture of 1248 ng of CMV-gvpA-IRES-BFP...

  4. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...subunit of DNA gyrase and trapping it in the cleaved complex. This results in DNA breakage and cell death. Conversely...often lost without plasmid addiction systems. For example, Cara Morin and James Kaper used the hok/sok system...K. Burley. "Crystal structure of the MazE/MazF complex: molecular bases of antidote-toxin recognition....

  5. How to Write a Scientific Review Article

    Type
    Blog Post
    ...Eventually I learned that it’s practical because there simply isn’t enough time to read every single paper. I... read the original reference for the data. For example, we summarized how frequently each member of a ...original paper if you wanted to learn more. Another example: we made a schematic of all the proteins in the...

  6. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...using viral LTRs as RNAP II promoters is commonly employed in lentiviral and retroviral constructs and we...speaking, promoters in bacteria are less diverse and complex, having fewer parts than those in eukaryotic cells...human ubiquitin C gene Constitutive  As the name implies, this promoter is ubiquitous. human beta actin...

  7. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...signaling — think of a mouse with an LED brain implant or a worm wiggling back and forth under blue light...have various disadvantages and limitations – for example, microtubule-destabilizing drugs are impossible... embryo (Aljiboury et al., 2023). It’s a great example of how optogenetics allows you to disrupt and study...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...longer RNAs (such as mRNAs for gene expression). Examples of commonly used RNA pol II promoters for Cas9...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...delivery and expression in planta using chitosan-complexed single-walled carbon nanotube carriers. Nat Nanotechnol...

  9. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918...BL21 strain and derivatives are the most common examples of the E. coli B strain. Common E. coli strains...vectors. Used by Addgene for pooled library amplification. F' mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96...

  10. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... NanoLuc® Luciferase-based tools are fantastic examples of technologies that can accelerate your research...endogenous protein tagging as it is only 19 kDa. An example is the CRISPaint-NLuc construct (Plasmid #67178...Another challenge to using FRET sensors comes when employing optogenetic regulators to initiate the event being...

  11. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...engineered ESCs), and chimeric adults mated to produce completely transgenic offspring. Although powerful, this... finally, we have a technology, CRISPR, that is simple in concept, straightforward to use, and robust ...enrichment and selection offered by mouse ESCs. For example, we often screen only 15-25 mice when generating...

  12. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...with and be inspired by the mentor’s successful example. For evidence of this, you needn’t look any further...cancer. She has guided me in my next steps. For example, how to get a fellowship to travel abroad, how ... a post-program self-assessment, participants completed follow-up self assessments and program evaluations...

  13. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...are working with proto-oncogenes or with human samples that have not been tested for HIV.  If not using...must be chosen carefully. Often researchers want a simple set-up where the gene of interest is expressed ...experience in transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly...

  14. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... favorite gene-of-interest, all the way to the complex multi-TU constructs that compose genetic circuits... backgrounds, MoClo can be invaluable for more complex editing and screening experiments in organisms ... blog Plasmids 101: Golden Gate Cloning High Complexity Golden Gate Cloning with NEB’s DAD New MoClo ...

  15. 5 Science Rap Videos & Their Creator: Tom McFadden

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    Blog Post
    ...Watson & Crick - Science History Rap Battle A great example of science story telling following the unfortunate...science educator looking for creative ways to implement the next generation science standards and communicate...

  18. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ..., in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine microenvironment... cells, an important immune cell type, in more complex and physiological microenvironments. These screens...

  19. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...’d like to take a moment to reflect on our accomplishments from 2022 and share what we’re looking forward...recommended uses and education content. This work exemplifies our long-standing commitment to be responsive...

  20. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    ...Plasmids" from these citing articles. What's this? Simply put, if the authors of a "Citing Article" have ...find the plasmids listed below the citation. An example of these "Associated Plasmids" is shown in the ...
Showing: 641 - 660 of 938 results