Addgene

We narrowed to 758 results for: ncl

Showing: 641 - 660 of 758 results

Results per page:

Plasmids 101: 5 factors to help you choose the right cloning method

Type

Blog Post

...mind that there are many other cloning methods - including some that rely solely on PCR, and a host of commercially...
10 Basic tips for mammalian cell culture

Type

Blog Post

...ll be brought under the hood with 70% ethanol, including your glove-covered hands! You should additionally...
xCas9: Engineering a CRISPR Variant with PAM Flexibility

Type

Blog Post

... xCas9 2.0-2.6. This second round of evolution included continuous outflow of phage, increasing stringency...
The Scientific Conference Poster Session: Tips for Success

Type

Blog Post

...Cancer Research Annual Meeting, you might consider including more background on how your virus of interest ...
Academic vs. Industry Postdocs

Type

Blog Post

...Many companies have industry postdoc positions, including AstraZeneca, Pfizer, Novartis, Biogen, Amgen, ...
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

Type

Blog Post

...all of the differentiated cells in an organism, including the embryonic and extraembryonic cell types. In...
Tips for a 1st time AAV user (by a Rookie AAV user)

Type

Blog Post

...… A no template control (NTC) should always be included. While the physical titer generated by qPCR is...
Polymerase Chain Reaction Overview and Applications

Type

Blog Post

...qPCR. qPCR is used in a variety of applications including gene expression profiling, studying copy number...
Hot Plasmids and Viral Preps - May 2021

Type

Blog Post

...our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral vectors. Both inhibitory...
Viral Vectors 101: Producing Your rAAV

Type

Blog Post

...can obtain excellent quality, high titer rAAV (including Addgene!), but you can also generate rAAVs in ...
Viral Vectors 101: Voltage Indicators

Type

Blog Post

...fluorescent output. The GEVI family of gadgets includes Ace, JEDI, and many more. They can be targeted...
Degrading DNA with Cascade-Cas3

Type

Blog Post

... 1: Schematic of Class 1 CRISPR types. Class 1 includes type I with a Cas3 effector, type III with a Cas10...
The AAVantages of AAV in CRISPR Screens

Type

Blog Post

...effectors allows for a wide variety of screens, including interference, activation, and knockout. Most...
Production of Virus in Insect Versus Mammalian Cells

Type

Blog Post

...be produced in multiple different cell types, including plants, mammals, and insects. In this post, we...
Magnetic Control of Proteins: More than a Dream

Type

Blog Post

...more. The team shares results early and often, including works-in-progress still in need of further study...
PhD Applications After COVID

Type

Blog Post

...virtual and hybrid options is more accessible, inclusive, and accommodating for everyone involved in the...
Experimenting with New Careers while in Grad School

Type

Blog Post

...multiple avenues for professional development including workshops and networking events. I took advantage...
Anatomy of a Plasmid Page at Addgene

Type

Blog Post

...additional resources provided by the depositor including genbank files and protocols here. Articles Citing...
Genome-wide Screening Using CRISPR

Type

Blog Post

...available in a 1-plasmid system, in which Cas9 is included on the gRNA-containing plasmid, or a 2-plasmid...
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Type

Blog Post

...historical studies of pathogen research have conclusively shown that barrier protocols are vulnerable ...

Showing: 641 - 660 of 758 results

Results per page:

Plasmids 101: 5 factors to help you choose the right cloning method

10 Basic tips for mammalian cell culture

xCas9: Engineering a CRISPR Variant with PAM Flexibility

The Scientific Conference Poster Session: Tips for Success

Academic vs. Industry Postdocs

Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

Tips for a 1st time AAV user (by a Rookie AAV user)

Polymerase Chain Reaction Overview and Applications

Hot Plasmids and Viral Preps - May 2021

Viral Vectors 101: Producing Your rAAV

Viral Vectors 101: Voltage Indicators

Degrading DNA with Cascade-Cas3

The AAVantages of AAV in CRISPR Screens

Production of Virus in Insect Versus Mammalian Cells

Magnetic Control of Proteins: More than a Dream

PhD Applications After COVID

Experimenting with New Careers while in Grad School

Anatomy of a Plasmid Page at Addgene

Genome-wide Screening Using CRISPR

Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections