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  1. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...combinatorial libraries, and high specificity has been reported for a number of targets. Due to their size and...ain't broke, don't fix it!  That being said, it's important to know all your options when planning and running...

  2. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...for IgG subtypes from different animals so it’s important to choose the reagent that works for your species...sure the staining you are seeing is real. It’s important to include a negative control using serum from...

  3. To Each HIS Own

    Type
    Blog Post
    ...tended to be very large and often required the incorporation of a cleavage site and a post-purification cleavage...very pure sample. Because binding strength is proportional to the length of the tag, with longer tags you...

  6. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...interacting proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster...identify highly biotinylated background proteins reported by other research groups that performed BirA*-...

  9. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate...be helpful having a coach that can offer extra support for issues of diversity in science.  A great person...

  10. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...lines can be found in the Cell Catalog on our data portal. The plasmids should be functional in many different...and the donor plasmid into the cells we use electroporation, a technique that briefly destabilizes the ...

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    ...grown in high concentrations of IL-3 show a high proportion of blue fluorescence, indicating cell proliferation...they could not proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804...

  17. Early Career Researcher Toolbox: Finding Relevant Papers

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    Blog Post
    ...about publications in the fields of research they support. Monitoring these types of accounts can help you...and figures using machine learning to extract important features such as influential citations, images...

  19. Antibodies 101: Choosing the Right Isotype

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    Blog Post
    ... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment...Why Choosing the Right Secondary Is of Primary Importance. In J. Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue...

  20. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...antibodies for IHC. Working with collaborators and support from the Brain Research Through Advancing Innovative...recombinant antibodies are a newer technology, it is important to show through qualitative data that they are...
Showing: 641 - 660 of 916 results