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...in the lab to deplete expression of proteins of interest. RNAi has even made it into agriculture as species-specific...

...functional mutations, antibiotic resistance, restriction sites used during cloning, and most importantly...

... a lox-STOP-lox cassette upstream of a gene of interest blocks transcription when Cre is absent. When ...

...understand human disease.  Outside the lab, he is interested in communicating science and in the intersection...

...Coach De La Professional but the website looks interesting and it is in English and Spanish. She is based...

... You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation Background... DNA: Contains the portion of DNA that we are interested in amplifying for analysis and manipulation. ...

...generate stable cell lines expressing a gene of interest from an integrated lentiviral vector....generate stable cell lines expressing a gene of interest from an integrated lentiviral vector. Unlike the...

...and I am excited to be a part of it! If you are interested to get involved by submitting data, sending antibodies...

...Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible Cas9 activity? Check out these...

...complex of TadA and dCas to the genomic DNA of interest  Figure 1. When ABEs are in action they...

... sequencing all incoming plasmids. If you are interested in requesting one of these plasmids, you can ...

...Genes allowing bacteria to survive toxins are the clearest example of a plasmid benefiting its host. For ...

...blots are a great tool to identify a protein of interest in a complicated solution like cell lysate. But...

...protein, StayGold. Although it quickly saw wide interest, its dimeric nature can interfere with some applications...

...development of therapies when expression must be restricted to specific circuits. Methods for targeting expression...

...and can be used to create fusions with a gene of interest. These plasmids will be most useful for constructing...

...Gibson cloning, ligation-independent cloning, restriction enzyme, TOPO cloning, unknown, or other. ...

...I would recommend conferences like Lab Robotic Interest Group (LRIG). There is so much to see and so many...

...titer, and successfully transducing a cell line of interest a lot can go wrong. We hope that we have provided...

... must be original (otherwise why would you be interested in it?) so make sure that comes across. Check...