Plasmid Tools for Microbiome Studies
Type
Blog Post
...transcripts of toxins and other transcripts of interest is a benefit of this method.
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CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...multiplexable RNA knockdown. Cas13 has also stirred up interest for its potential as a viral therapeutic that ... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog Post...These modifications result in conformationally restricted RNA which improves mismatch discrimination and... -
Antibodies 101: Affinity Reagents
TypeBlog Post...chains made by immune systems. But here, we're not interested in antibodies. Instead, we're going to focus ... -
Transferable Skills Guide: Public Speaking
TypeBlog Post..., much too late, that you labeled your gene of interest incorrectly on slides 8-16. Your notes disappear... -
All in a Twist: dsRNA
TypeBlog Post...in the lab to deplete expression of proteins of interest. RNAi has even made it into agriculture as species-specific... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...and thereby monitor autophagy of your protein of interest. Are you ready to try out Rosella? Both the cytosol-targeted... -
Addgene's Tips for Plasmid Quality Control
TypeBlog Post...functional mutations, antibiotic resistance, restriction sites used during cloning, and most importantly... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post... a lox-STOP-lox cassette upstream of a gene of interest blocks transcription when Cre is absent. When ... -
The Future of Research Symposium Boston 2015
TypeBlog Post...understand human disease. Outside the lab, he is interested in communicating science and in the intersection... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...Coach De La Professional but the website looks interesting and it is in English and Spanish. She is based... -
What is Polymerase Chain Reaction (PCR)
TypeProtocol... You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation Background... DNA: Contains the portion of DNA that we are interested in amplifying for analysis and manipulation. ... -
A Look at Addgene's QC Process
TypeBlog Post... sequencing all incoming plasmids. If you are interested in requesting one of these plasmids, you can ... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...Genes allowing bacteria to survive toxins are the clearest example of a plasmid benefiting its host. For ... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post...blots are a great tool to identify a protein of interest in a complicated solution like cell lysate. But... -
Lab Automation at Addgene
TypeBlog Post...I would recommend conferences like Lab Robotic Interest Group (LRIG). There is so much to see and so many... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible Cas9 activity? Check out these... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...complex of TadA and dCas to the genomic DNA of interest Figure 1. When ABEs are in action they... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...titer, and successfully transducing a cell line of interest a lot can go wrong. We hope that we have provided... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...and can be used to create fusions with a gene of interest. These plasmids will be most useful for constructing...