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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,052 results for: sta

Showing: 641 - 660 of 1052 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...usage has been limited by technical problems. For instance, it’s much more difficult to introduce a gene ...primary (hESCs) and cultured cells showed up to 70% resistance to diphtheria toxin, indicating a 70% frequency... adapted from D’Astolfo et al. In certain instances, the potential benefits of nonviral transduction...protein purification (once a protocol has been established) may be less laborious than viral purification...

  2. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...a great start to a mentoring relationship, but if you don’t take this past the chatting stage you will...list available from this post. Use an activity to start a discussion. The Forced Choice Analysis is a fun...clarify and discuss values. The activity is just the start – it is the discussion after that will lead to learning...

  3. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...range of characteristics (color, brightness, photostability, maturation, oligomerization), many of which...choosing a protein, we often direct them there as a starting point.  However, there is a great deal more information...database relating to that specific protein (for instance: EGFP).  Users can “favorite” certain proteins...of FPbase FPbase is still very much in the early stages of development, and there is plenty of room for...

  4. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...different water filtration system, environmental stability in an incubator), but there’s a lot you can do...other researchers to help them troubleshoot and understand. All of this communication and sharing fits together...seriously. That’s why at Cell Press we developed STAR Methods to improve the structure and transparency... of checklists and guidelines to help authors understand what they are expected to do. All of that helps...

  6. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...Standard AAV vectors are generated with a producer cell line like the widely used 293T. During vector...glioblastoma). They also found that exo-AAVs were more resistant to neutralizing anti-AAV antibodies. This is particularly...thought to enhance the production of exosomes or stabilize their structure or their interaction with AAVs...areas that are difficult to reach, and are more resistant to inactivation through anti-AAV antibodies. ...

  7. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...is fine for its goal of creating a choice of standardized constructs and tools for the rational and simple...Golden Gate Assembly also allows the creation of standardized DNA constructs, but its use of Type IIS restriction...polymerase used in the amplification. This tool allows instant updates as fragments are added, deleted, or reordered... in biofuel generation, green chemistry and understanding the minimal genome. Many of these techniques...

  8. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ... (Fig. 1). However, once thawed, there was a substantial reduction (from 7.4 to 5.0) in the pH of samples...antibodies, because of effects of pH on protein stability (Murphy et al., 2013). However, the pH changes...infectivity of the virus. However, there was a substantial reduction in infectivity after 3 weeks of exposure...der Geer P, Manning MC, Fitchmun MI. Protein instability following transport or storage on dry ice. Nat...

  9. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...give rise to a starfish shape. You can ask me about the different arms of this starfish sometime, but... plug-in called Maps.  Figure 1: See a starfish? This is actually a map of Joanne Kamen's LinkedIn... it is completely impossible for me or anyone to stay in touch with all of their connections on a regular...

  10. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...at odd times, new strategies can make socially distanced work more creative. An example could be making...signal the approach of a brain break, where everyone stands up, disappears from the video, and prepares a coffee...brainstorm on data in smaller groups, where for instance the presence of the PI in the main room inhibits...these weird times of shift working and social distancing. The take home message is the approaches/tools...

  11. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ...reagent is crucial for reproducibility because mistakes in this can lead to wasted time or retractions... the Addgene catalog. These IDs allow you to unmistakably identify a plasmid available through Addgene...all Addgene plasmids! In an effort to support standardized persistent identification of research reagents...Resource Identifiers (CURIEs) combined with well-established prefix resolution services such as Identifiers.org...

  12. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...efficiency of these editing reactions is low. Understanding the biological cause of this repair bias is ... reinforce a key scientific principle: that understanding a biological process in detail often suggests... efficiency of sequence replacement by HDR. Understanding Cas9 biophysics enhances HDR We first measured...released strand, 127bp in length, With 36bp on the PAM-distal side of the break and 91bp on the PAM-proximal ...

  13. How to Lead a Great Meeting

    Type
    Blog Post
    ... the purpose of the meeting: Begin meetings by stating “The purpose of this meeting is to _________." ... what you need to accomplish to get to the next stage, and then adjourn. Define the tone of the meeting...template for project meetings. Team Meetings: For standing team meetings, provide agenda sections for each...meeting. Accountability: At your next meeting, start by checking in on the action items from last meeting...

  14. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...promoters. Others are only active under specific circumstances. In this post, we’ll discuss inducible promoters...promoters, which can be switched from an OFF to an ON state, and how you might use these in your research. When...negative control. Positive inducible In the OFF state, the promoter is inactive because the activator ...transcription.     Negative inducible In the OFF state, the promoter is inactive because a bound repressor...

  15. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...at (and through) a couple of models. Before you start a new project with fluorescent proteins, the best...this series of posts are (i) brightness, (ii) photostability and (iii) aggregation tendency. Brightness...Without further information about properties like photostability or aggregation tendency, our choice would be...Goedhard and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

  16. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...reaction to understand if any of your buffer components could affect the reaction. For instance, sodium azide...either testing the antibody efficacy through a standard curve, another quick experiment, or using a different... to use, not sensitive to light in its inactive state, and cheap. Unless there’s a specific reason you...

  17. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...This method utilizes a packaging cell line that stably expresses Gag-Pol and/or Env, eliminating the need...packaging cell line developed by Garry Nolan at Stanford (Swift, 2001) contains Gag-Pol and either an ecotropic...differentiated (non-dividing) cells. Both lentiviruses and standard γ-retroviruses use the Gag, Pol, and Env genes...provirus. 3’ LTR Terminates transcription started by 5' LTR by the addition of a poly A tract just...

  18. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...virus. Since the WT sequence is relatively unstable, a more stable 130 bp version is used in most plasmids...recombinant-deficient bacterial strains such as NEB Stable, Stbl3, JC8111, SURE2, or XL10-Gold will help to...sequences can sometimes be removed to improve vector stability and sequencing results. Next-generation sequencing...

  19. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...infect cells and deliver cargo, it’s important to understand their differences to choose the best one for ...viruses Retroviruses are a family of viruses that stably integrate their genome into the host genome, setting...   Type of Virus Pros Cons Retrovirus Stable Gene Expression Broad Tropism Only Infects ...virus safety and viral applications to help you start (safely!) thinking about the possibilities. Ready...
Showing: 641 - 660 of 1052 results